Identification and optimization of carbon radicals on hydrated graphene oxide for ubiquitous antibacterial coatings

Patent metadata

Field	Value
Publication number	US-11208330-B2
Application number	US-201716348862-A
Country	US
Kind code	B2
Filing date	Nov 15, 2017
Priority date	Nov 16, 2016
Publication date	Dec 28, 2021
Grant date	Dec 28, 2021

Abstract

In various embodiments functionalized graphene oxide(s) are provided that demonstrate improved antimicrobial activity, where the graphene oxide(s) are functionalized to increase carbon radical (.C) density.

First claim

What is claimed is:

1. A hydrated graphene oxide (hGO) film disposed on a glass, metal, or polymer solid surface, where said hydrated graphene oxide film has increased carbon radical (.C) density as compared to pristine graphene oxide (GO), wherein said increased carbon radical density is characterized by:
i) an EPR spectrum showing a single symmetric resonance peak of π-conjugated carbon radical;
ii) an atomic percent concentration of epoxide (C—O—C) groups lower than pristine graphene oxide; and
iii) an atomic percent concentration of C—OH groups for said hGO at least twice the atomic percent concentration of C—OH groups on pristine graphene oxide as determined by X-ray photoelectron spectroscopy (XPS);
wherein said hydrated graphene oxide film disposed on said surface has increased antimicrobial activity as compared to a pristine graphene oxide film.

2. The graphene oxide of claim 1 , wherein the antimicrobial activity of said hydrated graphene oxide film is proportional to the carbon radical density.

3. The graphene oxide of claim 1 , wherein said hydrated graphene oxide film shows increased lipid membrane binding and/or induction of lipid peroxidation as compared to pristine graphene oxide.

4. The hydrated graphene oxide film of claim 1 , wherein:
said graphene oxide shows a carbon radical content as determined by electron paramagnetic resonance (EPR) with an absorbance peak area greater than about 15×10 6 ; and/or
said graphene oxide has an atomic % concentration of oxidized groups (C—OH) on the graphene oxide surface of about 9.9 or greater as determined by XPS.

5. The hydrated graphene oxide film of claim 1 , wherein:
said hydrated graphene oxide film is effective to kill gram negative bacteria; and/or
said hydrated graphene oxide film is effective to kill gram positive bacteria; and/or
said hydrated graphene oxide film is effective to kill E. coli.

6. The hydrated graphene oxide film of claim 1 , wherein:
said hydrated graphene oxide film is adsorbed to said solid surface; and/or
said hydrated graphene oxide film is spin-coated on said solid surface; and/or
said hydrated graphene oxide film is covalently attached to said solid surface.

7. The hydrated graphene oxide film of claim 6 , wherein said hydrated graphene oxide film ranges in thickness from about 1 nm up to about 50 nm.

8. The graphene oxide of claim 6 , wherein:
said hydrated graphene oxide film is attached to a surface that comprises a surface of a catheter; and/or
said hydrated graphene oxide film is attached to a surface of biological implant; and/or
said hydrated graphene oxide film is attached to a surface that comprises a surface of a biological implant selected from the group consisting of a dental implant, an encapsulated implantable drug delivery system, an implanted canula, and an orthopedic implant; and/or
said hydrated graphene oxide film is attached to a surface of an orthopedic implant; and/or
said hydrated graphene oxide film is attached to a surface of an orthopedic implant selected from the group consisting of an artificial joint, a bone screw, and a bone nail; and/or
said hydrated graphene oxide film is attached to a surface of an implant selected from the group consisting of an Austin-Moore prosthesis, Baksi's prosthesis, Charnley prosthesis, Condylar blade plate, Ender's nail, Grosse-Kempf (GK) nail, Harrington rod, Hartshill rectangle, Insall Burstein prosthesis, Richard N. W. Wohns interspinous implant, Kirschner wire, Kuntscher nail, Luque rod, Moore's pin, Neer's prosthesis, Rush nail, Smith Peterson (SP) nail, Smith Peterson nail with McLaughlin's plate, Seidel nail, Souter's prosthesis, Steffee plate, Steinmann pin, Swanson prosthesis, Talwalkar nail, and a Thompson prosthesis.

9. The hydrated graphene oxide film of claim 1 , wherein:
said hydrated graphene oxide film is a component of a composite or nanocomposite; and/or
said hydrated graphene oxide film is a component of a composite or nanocomposite selected from the group consisting of a metal composite or nanocomposite, metal oxide composite or nanocomposite, a polymer composite or nanocomposite, a quaternary phosphonium salt composite or nanocomposite, and a chelator composite or nanocomposite; and/or
said hydrated graphene oxide film is a component of a composite or nanocomposite that comprises a metal; and/or
said hydrated graphene oxide film is a component of a composite or nanocomposite that comprises a metal composite selected from group consisting of graphene oxide and silver, graphene oxide and copper, graphene oxide and gold, graphene oxide, and lanthanum; and/or
said hydrated graphene oxide film is a component of a composite or nanocomposite that comprises a metal oxide; and/or
said hydrated graphene oxide film is a component of a composite or nanocomposite that comprises a metal oxide selected from the group consisting of TiO 2 , ZnO, Fe 3 O 4 , and SnO 2 ; and/or
said hydrated graphene oxide film is a component of a composite or nanocomposite that comprises a polymer; and/or
said hydrated graphene oxide film is a component of a composite or nanocomposite that comprises a polymer selected from the group consisting of poly-N-vinyl carbazole (PVK), chitosan, and PVK.

10. The hydrated graphene oxide film of claim 1 , wherein said graphene oxide is additionally functionalized with polyethylenimine (PEI) and/or PEG, and/or PVA, and/or polydopamine.

11. The hydrated graphene oxide film of claim 1 , wherein said graphene oxide is attached to fibers or to nanofibers.

12. The hydrated graphene oxide film of claim 1 , wherein:
said hydrated graphene oxide film is a component of a three-component nanohybrid; and/or
said hydrated graphene oxide film is a component of a three-component nanohybrid that comprises a dimensional GO-Au@Ag nanohybrid; and/or
said hydrated graphene oxide film is a component of a three-component nanohybrid that comprises a GO-poly(acrylic acid)-Ag nanohybrid; and/or
said hydrated graphene oxide film is a component of a three-component nanohybrid that comprises a GO-polydopamine-Ag nanohybrid.

13. The hydrated graphene oxide film of claim 1 , wherein:
said hydrated graphene oxide film is a component of a tissue engineering scaffold; and/or
said hydrated graphene oxide film is a component of a tissue engineering scaffold that comprises a protein or carbohydrate scaffold; and/or
said hydrated graphene oxide film is a component of a tissue engineering scaffold that comprises one or more materials selected from the group consisting of collagen, chitosan, hyaluronic acid, fibrin, and gelatin; and/or
said hydrated graphene oxide film is a component of a tissue engineering scaffold that comprises a synthetic scaffold; and/or
said hydrated graphene oxide film is a component of a tissue engineering scaffold that comprises one or more materials selected from the group consisting of glycolic acid derivatives, lactic acid derivatives, and other polyester derivatives; and/or
said hydrated graphene oxide film is a component of a tissue engineering scaffold that comprises a copolymer of 1-lactide and epsilon-caprolactone; and/or
said hydrated graphene oxide film is a component of a tissue engineering scaffold that comprises a polyglycolic acid mesh coated with a copolymer of poly[epsilon-caprolactone-1-lactide].

14. The hydrated graphene oxide film of claim 1 , wherein:
said hydrated graphene oxide film is incorporated into a bandage and/or wound dressing; or
said hydrated graphene oxide film is incorporated into a water filter.

15. The hydrated graphene oxide film of claim 1 , wherein said hydrated g

Assignees

• Univ California
• Univ Northwestern

Inventors

• Nel Andre E
• Xia Tian
• Li Ruibin
• Hersam Mark C
• Mansukhani Nikhita D
• Guiney Linda

Classifications

• C01B32/198 Primary

  Graphene oxide · CPC title

• A01N43/90

  having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system · CPC title

• A61L27/08

  Carbon {; Graphite} · CPC title

• C01B32/182

  Graphene · CPC title

• A01N59/00

  Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds · CPC title