Biosensor array for the detection of analytes

Patent metadata

Field	Value
Publication number	US-11185263-B2
Application number	US-201816198363-A
Country	US
Kind code	B2
Filing date	Nov 21, 2018
Priority date	Nov 21, 2017
Publication date	Nov 30, 2021
Grant date	Nov 30, 2021

Abstract

Embodiments of the present disclosure relate generally devices for detecting analytes in a subject. More particularly, the present disclosure provides a biosensor array for detecting analytes in a subject. Embodiments of the present disclosure include a biosensor array comprising a plurality of sensor cells for detecting an analyte in a subject. In accordance with these embodiments, the plurality of sensor cells comprise at least one electrode, at least one antibody immobilized on a surface of the at least one electrode, and a biodegradable coating in contact with the at least one antibody.

First claim

What is claimed is:

1. An implantable biosensor for detecting insulin comprising:
an implantable biosensor array comprising a plurality of sensor cells comprising at least one working electrode, a counter electrode, and a reference electrode;
one or more insulin-specific antibodies immobilized on a surface of the at least one working electrode;
each sensor cell containing a biodegradable gelatin coating of varying thicknesses in direct contact with the one or more insulin-specific antibodies; and
where upon subcutaneous implantation in a subject, the various thicknesses of biodegradable gelatin coating degrade at different rates over a period of 1 week to 6 months thereby exposing the insulin-specific antibodies at varying time points.

2. The biosensor of claim 1 , wherein the biosensor array comprises at least 72 sensor cells.

3. The biosensor of claim 1 , wherein the biosensor array comprises 96 sensor cells.

4. The biosensor of claim 1 , wherein the biosensor array is about 0.1 mm-10 mm thick.

5. The biosensor of claim 1 , wherein the biosensor array is about 0.1 mm-5 mm thick.

6. A method for detecting insulin, the method comprising:
subcutaneously implanting the biosensor of claim 1 in a subject;
after the biodegradable gelatin coating degrades over the period of 1 week to 6 months exposing the insulin-specificantibody site, detecting insulin in the subject.

Assignees

• Labelle Jeffrey
• Probst David
• Mu Bin
• Univ Arizona State

Inventors

• Labelle Jeffrey
• Probst David
• Mu Bin

Classifications

• A61B5/14546

  for measuring analytes not otherwise provided for, e.g. ions, cytochromes · CPC title

• A61B5/14507

  specially adapted for measuring characteristics of body fluids other than blood (devices for taking samples of body liquids A61B10/0045) · CPC title

• G01N2333/62

  Insulins · CPC title

• A61B5/1486

  using enzyme electrodes, e.g. with immobilised oxidase · CPC title

• A61B5/14735

  comprising an immobilised reagent · CPC title