Fungal resistant plants expressing RLK2

Patent metadata

Field	Value
Publication number	US-11180772-B2
Application number	US-201816049245-A
Country	US
Kind code	B2
Filing date	Jul 30, 2018
Priority date	Aug 9, 2012
Publication date	Nov 23, 2021
Grant date	Nov 23, 2021

Abstract

The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an RLK2 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an RLK2 protein.

First claim

The invention claimed is:

1. A method for preventing, reducing, or delaying Phakopsora infection in a soybean plant, a soybean plant part, or a soybean plant cell, the method comprising:
providing a transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell comprising an exogenous nucleic acid encoding a receptor-like kinase 2 (RLK2) protein comprising an amino acid sequence having at least 96% identity with SEQ ID NO: 2, wherein the RLK2 protein confers increased resistance against Phakopsora thereto in comparison to a wild type soybean plant, wild type soybean plant part, or wild type soybean plant cell, wherein the exogenous nucleic acid encoding the RLK2 protein is operably linked to a heterologous promoter; and
growing the transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell in the presence of a fungal pathogen of the genus Phakopsora , wherein Phakopsora infection is prevented, reduced, or delayed in the transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell as compared to a wild type soybean plant, wild type soybean plant part, or wild type soybean plant cell.

2. The method of claim 1 , wherein the exogenous nucleic acid encodes an RLK2 protein comprising an amino acid sequence having at least 97% identity to SEQ ID NO: 2.

3. The method of claim 1 , wherein the exogenous nucleic acid encodes an RLK2 protein comprising an amino acid sequence having at least 98% identity to SEQ ID NO: 2.

4. The method of claim 1 , wherein the exogenous nucleic acid encodes an RLK2 protein comprising an amino acid sequence having at least 99% identity to SEQ ID NO: 2.

5. The method of claim 1 , wherein the exogenous nucleic acid encodes an RLK2 protein comprising an amino acid sequence having 100% identity to SEQ ID NO: 2.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Schultheiss Holger
• Mentzel Tobias
• Flachmann Ralf

Classifications

• C12N15/8282 Primary

  for fungal resistance · CPC title

• C12N9/1205

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title