Fungal resistant plants expressing RLK2

Patent metadata

Field	Value
Publication number	US-10066239-B2
Application number	US-201314419330-A
Country	US
Kind code	B2
Filing date	Aug 1, 2013
Priority date	Aug 9, 2012
Publication date	Sep 4, 2018
Grant date	Sep 4, 2018

Abstract

The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an RLK2 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an RLK2 protein.

First claim

The invention claimed is:

1. A method for preventing, reducing, or delaying Phakopsora infection in a soybean plant, a soybean plant part, or a soybean plant cell, the method comprising: providing a transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell having an exogenous nucleic acid encoding a receptor-like kinase 2 (RLK2) protein comprising an amino acid sequence having at least 74% identity to SEQ ID NO: 2, wherein the RLK2 protein confers increased resistance against Phakopsora thereto in comparison to a wild type soybean plant, wild type soybean plant part or wild type soybean plant cell, wherein the exogenous nucleic acid encoding the RLK2 protein is operably linked to a heterologous promoter; and
growing the transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell in the presence of a fungal pathogen of the genus Phakopsora , wherein Phakopsora infection is prevented, reduced, or delayed in the transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell as compared to a wild type soybean plant, wild type soybean plant part, or wild type soybean plant cell.

2. The method of claim 1 , wherein the promoter is a constitutive promoter, a pathogen-inducible promoter, a mesophyll-specific promoter or an epidermis-specific promoter.

3. The method of claim 1 , wherein the exogenous nucleic acid encodes an RLK2 protein comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 2.

4. The method of claim 1 , wherein the exogenous nucleic acid encodes an RLK2 protein comprising an amino acid sequence having at least 90% identity to SEQ ID NO: 2.

5. The method of claim 1 , wherein the exogenous nucleic acid encodes an RLK2 protein comprising an amino acid sequence having at least 95% identity to SEQ ID NO: 2.

6. A recombinant vector construct comprising in operable linkage:
(a) a nucleic acid encoding an RLK2 protein comprising an amino acid sequence having at least 74% identity with SEQ ID NO: 2
(b) a heterologous, fungal-inducible promoter and
(c) a transcription termination sequence,
wherein expression of said recombinant vector construct in a plant, plant part, or plant cell confers increased resistance against Phakopsora thereto in comparison to a wild type plant, wild type plant part, or wild type plant cell.

7. A method for the production of a transgenic plant, transgenic plant part, or transgenic plant cell having increased resistance against Phakopsora , said method comprising:
(a) introducing the recombinant vector construct of claim 6 into a plant, a plant part, or a plant cell; and
(b) generating a transgenic plant, transgenic plant part, or transgenic plant cell from the plant, plant part or plant cell.

8. The method of claim 7 , further comprising the step of harvesting the seeds of the transgenic plant and planting the seeds and growing the seeds to plants, wherein the grown plants comprise the recombinant vector construct.

9. A transgenic plant, transgenic plant part, or transgenic plant cell comprising the recombinant vector construct of claim 6 .

10. A harvestable part of the transgenic plant of claim 9 , wherein the harvestable part comprises the recombinant vector construct.

11. A product derived from the plant of claim 9 , wherein the product comprises the recombinant vector construct.

12. The product of claim 11 , comprising soybean meal or soy oil.

13. A method for the production of a product, said method comprising:
a) growing the plant of claim 9 ; and
b) producing said product from the plant and/or a part thereof,
wherein the product obtained by said method comprises the recombinant vector construct.

14. The method of claim 13 , wherein the product is produced from seeds of the plant.

15. The method of claim 13 , wherein the product is meal or oil.

16. A method for breeding a fungal resistant plant, said method comprising:
(a) crossing the plant of claim 9 with a second plant;
(b) obtaining seed from the cross of step (a);
(c) planting said seeds and growing the seeds to plants; and
(d) selecting from the plants produced in step (c) plants expressing the RLK2 protein.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Schultheiss Holger
• Mentzel Tobias
• Flachmann Ralf

Classifications

• C12N9/1205

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title

• C12N15/8282 Primary

  for fungal resistance · CPC title