Compact microscope

Patent metadata

Field	Value
Publication number	US-11169366-B2
Application number	US-201916405226-A
Country	US
Kind code	B2
Filing date	May 7, 2019
Priority date	Oct 25, 2013
Publication date	Nov 9, 2021
Grant date	Nov 9, 2021

Abstract

A compact microscope including an enclosure, a support element, a primary optical support element located within the enclosure and supported by the support element, at least one vibration isolating mount between the support element and the primary optical support element, an illumination section, an objective lens system, a sample stage mounted on the primary optical support element, an illumination optical system to direct an illumination light beam from the illumination section to the sample stage, and a return optical system to receive returned light from sample stage and transmit returned light to a detection apparatus, wherein the illumination optical system and return optical system are mounted on the primary optical support element.

First claim

We claim:

1. A microscope system comprising:
a primary optical support element; and
a microscope focus control system comprising:
a movable sample stage;
an objective lens system;
wherein the objective lens system is mounted on the primary optical support element; and
a focus stability beam optical system to direct a focus stability beam to the objective lens system, the focus stability beam optical system comprising:
a focus stability beam laser source to generate the focus stability beam;
a focus stability beam optical fibre to transmit the focus stability beam to the objective lens system; and
a focus stability beam optical fibre connection,
wherein the focus stability beam optical fibre extends non-linearly between the focus stability beam laser source and the focus stability beam optical fibre connection,
wherein the focus stability beam optical fibre is securely attached to the primary optical support element via the focus stability beam fibre connection;

wherein the microscope system further comprises:
an illumination source module, comprising at least one illumination light beam source which is physically separate to the focus stability beam laser source, said illumination light beam source arranged to generate an illumination light beam for imaging a sample;

wherein the microscope focus control system further comprises:
an imaging apparatus comprising a detector, wherein the detector is arranged to:
image the sample; and
image the focus stability beam;

a return optical system to return light to the imaging apparatus; and
a control system operable to receive a reference image of the focus stability beam from the objective lens system, receive a subsequent image of the focus stability beam, and control the sample stage in accordance with the reference image and subsequent image,

wherein the illumination source module is arranged to direct the illumination light beam to the objective lens system.

2. A microscope system according to claim 1 wherein the control system is operable to control the movable sample stage such that a subsequent image matches the reference image.

3. The microscope system as claimed in claim 1 , wherein the subsequent image of the focus stability beam is received from the imaging apparatus.

4. The microscope system as claimed in claim 1 , wherein the focus stability beam optical system comprises a lens to collimate the focus stability beam and one or more mirrors to direct the focus stability beam to the objective lens system.

5. The microscope system as claimed in claim 1 , wherein the control system is operable to compare the reference image with a subsequent image.

6. The microscope system as claimed in claim 1 , wherein the control system is operable to store a plurality of reference images each corresponding to a different position of the movable sample stage and to control the movable sample stage such that a subsequent image corresponds to one of the plurality of reference images.

7. A method of operating a microscope system, the microscope system comprising:
a primary optical support element;
an objective lens system mounted on the primary optical support element;
the method comprising:
storing a reference image of a focus stability beam;
generating the focus stability beam using a focus stability beam laser source;
transmitting the focus stability beam via a focus stability beam optical fibre to the objective lens system of a microscope, the focus stability beam optical fibre extending non-linearly between the focus stability beam laser source and a focus stability beam optical fibre connection, the focus stability beam optical fibre being securely attached to the primary optical support element via the focus stability beam optical fibre connection;
imaging the focus stability beam at a detector arranged to image the focus stability beam and a sample;
receiving a subsequent image of the focus stability beam;
controlling a sample stage of the microscope in accordance with the reference image and subsequent image;
transmitting an illumination light beam from an illumination light beam source, separate from the focus stability beam source, to the objective lens system; and
imaging the sample at the detector.

8. A method according to claim 7 , comprising controlling the sample stage such that a subsequent image matches the reference image.

9. The method as claimed in claim 7 , wherein the method comprises receiving the subsequent image of the focus stability beam from an imaging apparatus.

10. The method as claimed in claim 7 , wherein the method comprises collimating the focus stability beam using a lens and directing the focus stability beam to the objective lens system via one or more mirrors.

11. The method as claimed in claim 7 , wherein the method comprises comparing the reference image with a subsequent image.

12. The method as claimed in claim 7 , wherein the method comprises storing a plurality of reference images each corresponding to a different position of the sample and controlling the sample stage such that a subsequent image corresponds to one of the plurality of reference images.

Assignees

• Univ Oxford Innovation Ltd

Inventors

• Kapanidis Achillefs
• Jing Bo
• Crawford Robert

Classifications

• G02B21/362

  Mechanical details, e.g. mountings for the camera or image sensor, housings (G02B21/364 takes precedence) · CPC title

• G02B21/244

  using image analysis techniques · CPC title

• G02B21/36

  arranged for photographic purposes or projection purposes (G02B21/18 takes precedence){or digital imaging or video purposes including associated control and data processing arrangements (image data processing per se G06T)} · CPC title

• G02B21/0008 Primary

  Microscopes having a simple construction, e.g. portable microscopes · CPC title

• G02B21/0088

  Inverse microscopes · CPC title