Compact microscope

Patent metadata

Field	Value
Publication number	US-10330904-B2
Application number	US-201415031807-A
Country	US
Kind code	B2
Filing date	Oct 27, 2014
Priority date	Oct 25, 2013
Publication date	Jun 25, 2019
Grant date	Jun 25, 2019

Abstract

A compact microscope including an enclosure, a support element, a primary optical support element located within the enclosure and supported by the support element, at least one vibration isolating mount between the support element and the primary optical support element, an illumination section, an objective lens system, a sample stage mounted on the primary optical support element, an illumination optical system to direct an illumination light beam from the illumination section to the sample stage, and a return optical system to receive returned light from sample stage and transmit returned light to a detection apparatus, wherein the illumination optical system and return optical system are mounted on the primary optical support element.

First claim

The invention claimed is:

1. A compact microscope, comprising;
an enclosure,
a support element,
a primary optical support element located within the enclosure and supported by the support element,
at least one vibration isolating mount between the support element and the primary optical support element,
a moveable sample stage and an objective lens system mounted on the primary optical support element,
a sample holder mounted on a face of the moveable sample stage, and
a return optical system to receive returned light from the sample stage and transmit returned light to a detection apparatus,
wherein the return optical system is mounted on the primary optical support element, wherein the enclosure completely encloses the optical paths of the microscope, and wherein the enclosure and/or the support element comprise vibration isolating material, and
wherein the moveable sample stage is connected to the primary optical support element by a fixed mounting point at the face of the moveable sample stage on which the sample holder is mounted and by a slidable mounting point remote from the face of the moveable sample stage.

2. A compact microscope according to claim 1 , having an illumination section and an illumination optical system to direct an illumination light beam from the illumination section to the moveable sample stage wherein the illumination optical system is mounted on the primary optical support element.

3. A compact microscope according to claim 2 , wherein at least part of the illumination optical system and the return optical system are located in different planes of the compact microscope.

4. A compact microscope according to claim 3 , wherein the detection apparatus is supported by the primary optical support element.

5. A compact microscope according to claim 2 , wherein at least part of the illumination optical system and the return optical system comprises a secondary optical support element supported by the primary optical support element.

6. A compact microscope according to claim 2 , wherein the illumination section comprises a connection to receive an optical fibre connected to a light source.

7. A compact microscope according to claim 2 , wherein the detection apparatus comprises a photodetector.

8. A compact microscope according to claim 2 , wherein the detection apparatus comprises an imaging apparatus.

9. A compact microscope according to claim 2 , wherein the illumination optical system comprises beam shaping optics to control the shape of an illumination light beam.

10. A compact microscope according to claim 9 , wherein the illumination optical system comprises at least one aperture to control the shape of an illumination light beam.

11. A compact microscope according to claim 2 , wherein the return optical system is operable to separate returned light into at least a first wavelength band and a second wavelength band.

12. A compact microscope according to claim 11 , wherein the detection apparatus comprises a photodetector and wherein the return optical system directs returned light in a first wavelength band to a first area of the imaging apparatus and returned light in a second wavelength band to a second area of the imaging apparatus.

13. A microscope system comprising a compact microscope according to claim 2 , and an illumination source module, the illumination optical system of the compact microscope and the illumination source module being connected by an optical fibre.

14. A microscope system according to claim 13 , wherein the illumination source module comprises a laser source.

15. A microscope system according to claim 14 , wherein the illumination source module comprises a first laser source to generate a first illumination light beam having a first wavelength and a second laser source to generate a second illumination light beam having a second wavelength, and a beam combination optical system to transmit the first illumination light beam and second illumination light beam to the optical fibre.

16. A microscope system according to claim 14 , further comprising a focus stability beam optical system to direct a focus stability beam to the objective lens system and wherein the illumination source module comprises a focus stability beam laser source and a focus stability beam optical fibre to transmit the focus stability beam to the compact microscope.

17. A compact microscope according to claim 1 , further comprising a focus stability beam optical system to direct a focus stability beam to the objective lens system.

18. A compact microscope according to claim 17 , comprising a focus controller having a reference image of the focus stability beam, and being operable to receive a subsequent image of the focus stability beam from the objective lens system and control the moveable sample stage in accordance with the reference image and subsequent image.

19. A compact microscope according to claim 18 , wherein the subsequent image of the focus stability beam is received from the light detection apparatus.

Assignees

• Isis Innovation
• Univ Oxford Innovation Ltd

Inventors

• Kapanidis Achillefs
• Jing Bo
• Crawford Robert

Classifications

• G02B21/244

  using image analysis techniques · CPC title

• G02B21/0008 Primary

  Microscopes having a simple construction, e.g. portable microscopes · CPC title

• G02B27/646

  compensating for small deviations, e.g. due to vibration or shake (movement of one or more optical elements for control of motion blur in cameras, projectors or printers G03B2205/0007; image stabilisation in cameras peculiar to the presence or use of an electronic image sensor H04N23/68) · CPC title

• G02B21/362

  Mechanical details, e.g. mountings for the camera or image sensor, housings (G02B21/364 takes precedence) · CPC title

• G02B21/0088

  Inverse microscopes · CPC title