Coalescence of droplets

Patent metadata

Field	Value
Publication number	US-11141730-B2
Application number	US-201414896218-A
Country	US
Kind code	B2
Filing date	Jun 12, 2014
Priority date	Jun 14, 2013
Publication date	Oct 12, 2021
Grant date	Oct 12, 2021

Abstract

The present invention generally relates to microfluidics, and, in particular, to systems and methods for coalescing or fusing droplets. In certain aspects, two or more droplets within a microfluidic channel are brought together and caused to coalesce without using electric fields or charges. For example, in certain embodiments, droplets stabilized with a surfactant may be disrupted, e.g., by exposing the droplets to a solvent able to alter the surfactant, which may partially destabilize the droplets and allow them to coalesce. In some instances, the droplets may also be physically disrupted to facilitate coalesce. In addition, in some cases, the positions of one or more droplets may be controlled within a channel using a groove in a wall of the channel. For example, a droplet may at least partially enter the groove such that the position of the droplet is at least partially controlled by the groove.

First claim

What is claimed is:

1. A method, comprising:
flowing a first fluidic droplet and a second fluidic droplet in a liquid within a microfluidic channel, the first fluidic droplet and the second fluidic droplet each stabilized in the liquid using a surfactant, wherein the first fluidic droplet and the second fluidic droplet are each controlled within the microfluidic channel using a groove in a wall of the microfluidic channel, wherein said groove steers said first and second fluidic droplets to a lower area of said wall;
exposing the first fluidic droplet and/or the second fluidic droplet to a solvent able to alter interfacial tension of the surfactant, wherein said solvent at least partially dissolves said surfactant; and
causing the first fluidic droplet and the second fluidic droplet to merge into a combined droplet without using electric fields or charges,
wherein the first fluidic droplet and/or the second fluidic droplet are altered or disrupted when exposing the droplets to the solvent, wherein the amount of disruption is controlled.

2. The method of claim 1 , wherein causing the first fluidic droplet and the second fluidic droplet to merge comprises physically disrupting the first fluidic droplet and/or the second fluidic droplet.

3. The method of claim 1 , wherein causing the first fluidic droplet and the second fluidic droplet to merge comprises altering flow of the first fluidic droplet and/or the second fluidic droplet within the microfluidic channel.

4. The method of claim 1 , wherein causing the first fluidic droplet and the second fluidic droplet to merge comprises contacting the first fluidic droplet with the second fluidic droplet.

5. The method of claim 1 , wherein causing the first fluidic droplet and the second fluidic droplet to merge comprises causing the first fluidic droplet and/or the second fluidic droplet to encounter an obstruction within the microfluidic channel.

6. The method of claim 1 , comprising causing the first fluidic droplet and the second fluidic droplet to merge into a combined droplet without creating an electrical charge on first fluidic droplet or the second fluidic droplet.

7. The method of claim 1 , further comprising causing the first fluidic droplet and the second fluidic droplet to contact a wall of the microfluidic channel.

8. The method of claim 7 , wherein the first fluidic droplet and the second fluidic droplet contact the wall of the microfluidic channel prior to exposing the first fluidic droplet and/or the second fluidic droplet to the solvent.

9. The method of claim 1 , further comprising diluting the solvent after causing the first fluidic droplet and the second fluidic droplet to merge.

10. The method of claim 1 , further comprising applying the surfactant to the combined droplet after causing the first fluidic droplet and the second fluidic droplet to merge into the combined droplet.

11. The method of claim 1 , further comprising applying a second surfactant to the combined droplet after causing the first fluidic droplet and the second fluidic droplet to merge into the combined droplet.

12. The method of claim 1 , wherein the solvent alters interfacial tension of the surfactant by at least about 10 mN/m.

13. The method of claim 1 , wherein the solvent and the surfactant are substantially miscible at a first weight ratio and precipitate at a second weight ratio.

14. The method of claim 1 , comprising flowing a plurality of first fluidic droplets and a second plurality of fluidic droplets and causing the first fluidic droplets and the second fluidic droplets to merge.

15. The method of claim 1 , wherein the first fluidic droplet has a volume no more than about 10 times bigger than a volume of the second fluidic droplet.

16. A method, comprising:
flowing a first fluidic droplet and a second fluidic droplet in a liquid in a microfluidic channel, the microfluidic channel containing a groove defined in a wall of the microfluidic channel, the groove having a cross-sectional area that decreases in a downstream direction of the microfluidic channel, wherein a size difference of the cross section areas is at least about 5%; and
causing the first fluidic droplet and the second fluidic droplet to move towards a wall of the microfluidic channel via interaction of the respective fluidic droplet with the groove;
exposing the first fluidic droplet and/or the second fluidic droplet to a solvent able to alter interfacial tension of the surfactant, wherein said solvent at least partially dissolves said surfactant; and
causing the first fluidic droplet and the second fluidic droplet to merge into a combined droplet.

17. The method of claim 16 , wherein upon entrance of the first or second droplets into the groove, the droplets change velocity within the channel.

18. The method of claim 16 , further comprising flowing a second fluidic droplet within the microfluidic channel.

19. The method of claim 18 , wherein the fluidic droplet and the second fluidic droplet are brought into contact with each other upon interaction of at least one of the fluidic droplet and the second fluidic droplet with the groove.

Assignees

• Harvard College

Inventors

• Weitz David A
• Kodger Thomas E
• Aubrecht Donald
• Akartuna Ilke

Classifications

• B01F33/3021

  the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets · CPC title

• B01F25/433

  Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles · CPC title

• B01J2219/00903

  Segmented flow · CPC title

• B01J2219/00896

  Changing inlet or outlet cross-section, e.g. pressure-drop compensation · CPC title

• B01J2219/00855

  Surface features · CPC title