Methods for saccharifying a starch subtrate

Patent metadata

Field	Value
Publication number	US-11130975-B2
Application number	US-202016745760-A
Country	US
Kind code	B2
Filing date	Jan 17, 2020
Priority date	Dec 19, 2014
Publication date	Sep 28, 2021
Grant date	Sep 28, 2021

Abstract

Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of Fusarium verticillioides glucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.

First claim

What is claimed is:

1. A method for saccharifying a starch substrate comprising at least 33.5% dry solids (ds) to produce glucose, comprising contacting the starch substrate with a) a glucoamylase comprising Fv-GA (SEQ ID NO: 1); and b) at least one additional glucoamylase comprising Afu-GA (SEQ ID NO: 3) or An-GA (SEQ ID NO: 7).

2. The method of claim 1 , wherein the additional glucoamylase is Afu-GA (SEQ ID NO: 3).

3. The method of claim 1 , wherein the additional glucoamylase is An-GA (SEQ ID NO: 7).

4. The method of claim 1 , wherein saccharifying the starch substrate results in a high glucose syrup.

5. The method of claim 4 , wherein the high glucose syrup comprises an amount of glucose selected from the list consisting of at least 95.5% glucose, at least 95.6% glucose, at least 95.7% glucose, at least 95.8% glucose, at least 95.9% glucose, at least 96% glucose, at least 96.1% glucose, at least 96.2% glucose, at least 96.3% glucose, at least 96.4% glucose, at least 96.5% glucose and at least 97% glucose.

6. The method of claim 4 , further comprising fermenting the high glucose syrup to an end product.

7. The method of claim 6 , wherein saccharifying and fermenting are carried out as a simultaneous saccharification and fermentation (SSF) process.

8. The method of claim 6 , whierein the end product is alcohol.

9. The method of claim 8 , wherein the end product is ethanol.

10. The method of claim 1 , wherein the starch substrate is about, 5% to 99%, 15% to 50% or 40-99% dry solid (DS).

11. The method of claim 1 , wherein each glucoamylase is dosed at a range of about 0.01 to about 10 glucoamylase unit (GAU) per gram of dry solid starch (dss).

12. The method of claim 1 , wherein the starch substrate is selected from wheat, barley, corn, rye, rice, sorghum, bran, cassava, milo, millet, potato, sweet potato, tapioca, and any combination thereof.

13. The method of claim 1 , wherein the starch substrate comprises liquefied starch, gelatinized starch, or granular starch.

14. The method of claim 1 , further comprising adding a hexokinase, a xylanase, a glucose isomerase, a xylose isomerase, a phosphatase, a phytase, a pullulanase, a β amylase, an α-amylase, a protease, a cellulase, a hemicellulase, a lipase, a cutinase, a trehalase, an isoamylase, a redox enzyme, an esterase, a transferase, a pectinase, a hydrolase, an alpha-glucosidase, an beta-glucosidase, or a combination thereof to the starch substrate.

15. The method of claim 14 , wherein a pullulanase is further added.

Assignees

• Danisco Us Inc

Inventors

• Sharma Vivek
• Teunissen Paula Johanna Maria

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12G3/023

  of botanical family Solanaceae, e.g. potato · CPC title

• C12Y302/01003

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title