Sample processing for microscopy

Patent metadata

Field	Value
Publication number	US-11112593-B2
Application number	US-202016804957-A
Country	US
Kind code	B2
Filing date	Feb 28, 2020
Priority date	Apr 8, 2016
Publication date	Sep 7, 2021
Grant date	Sep 7, 2021

Abstract

In some instances, an apparatus can include a light sensitive imaging sensor having a surface to receive a fluid sample, a body to be moved relative to the light sensitive imaging sensor and having a surface to touch a portion of the fluid sample, and a carrier to move the body toward the surface of the light sensitive imaging sensor to cause the surface of the body to touch the portion of the fluid sample, so that as the surface of the body touches the portion of the fluid, the surface of the body (i) is parallel to the surface of the light sensitive imaging sensor, and (ii) settles on top of the fluid sample independently of motion of the carrier.

First claim

What is claimed is:

1. An apparatus comprising:
a light sensitive imaging sensor having a surface to receive a fluid sample in contact with the surface of the light sensitive imaging sensor;
a body to be moved relative to the light sensitive imaging sensor and having an exposed surface facing toward the surface of the light sensitive imaging sensor and configured to touch a portion of the fluid sample when the fluid sample is in contact with the surface of the light sensitive imaging sensor and between the surface of the light sensitive imaging sensor and the body; and
a carrier configured to move the body toward the surface of the light sensitive imaging sensor to cause the exposed surface of the body to touch the portion of the fluid sample and then to become supported by the fluid sample, wherein the carrier is configured such that, as the body becomes supported by the fluid sample, the body becomes unsupported by the carrier and separates from the carrier.

2. The apparatus of claim 1 , wherein at least a portion of the body is transparent.

3. The apparatus of claim 1 , wherein the surface of the light sensitive imaging sensor to receive the fluid sample comprises a hydrophilic coating.

4. The apparatus of claim 3 , comprising a base comprising the light sensitive imaging sensor,
wherein a surface of the base surrounding the surface of the light sensitive imaging sensor to receive the fluid sample comprises a hydrophobic coating.

5. The apparatus of claim 1 , wherein the exposed surface of the body comprises a hydrophilic coating.

6. The apparatus of claim 1 , further comprising a sample delivery component for preparing and delivering the fluid sample to the surface of the light sensitive imaging sensor.

7. The apparatus of claim 6 , wherein the sample delivery component comprises at least two volumetric capillary tubes, a nozzle for mixing fluids within the at least two volumetric capillary tubes, and an output tip through which the fluid sample is delivered to the surface of the light sensitive imaging sensor.

8. The apparatus of claim 1 , wherein the carrier comprises an extension on which the body rests.

9. The apparatus of claim 1 , in which the body comprises one or more elements configured to guide motion of the body as the carrier moves the body toward the surface of the light sensitive imaging sensor.

10. The apparatus of claim 1 , further comprising a device that moves the carrier to adjust a vertical distance between the exposed surface of the body and the surface of the light sensitive imaging sensor that receives the fluid sample.

11. A method comprising
supporting a body on a carrier; and
moving the carrier to move the body toward a fluid sample that is on a surface of a light sensitive imaging sensor and is between the body and the light sensitive imaging sensor, so that, as the body touches the fluid sample, the body a) becomes supported by the fluid sample, and b) displaces, from the surface of the light sensitive imaging sensor, a portion of the fluid sample, wherein, as the body becomes supported by the fluid sample, the body becomes unsupported by the carrier and separates from the carrier.

12. The method of claim 11 , wherein supporting the body on the carrier comprises placing the body on the carrier such that a center of the body is vertically aligned with a center of the light sensitive imaging sensor.

13. A point-of-care system comprising:
a sample processing compartment including
a base comprising a carrier and a light sensitive imaging sensor having a surface to receive a fluid sample, wherein the surface of the light sensitive imaging sensor is disposed inside a recess defined by the base, and
a body configured to be held by the carrier with the fluid sample between the body and the light sensitive imaging sensor, the body comprising a protruding element that extends toward the light sensitive imaging sensor when the body is held by the carrier, wherein the protruding element is sized and shaped to fit inside the recess;

a device coupler configured to couple electronically to a mobile device and to transmit, to the mobile device, electronic communications corresponding to signals derived from the light sensitive imaging sensor; and
a housing configured to hold the sample processing compartment and the device coupler.

14. The system of claim 13 , wherein the surface of the light sensitive imaging sensor to receive the fluid sample comprises a hydrophilic coating.

15. The system of claim 13 , wherein a surface of the protruding element facing the light sensitive imaging sensor when the body is held by the carrier comprises a hydrophilic coating.

16. The system of claim 13 , further comprising a sample delivery component for preparing and delivering the fluid sample to the surface of the light sensitive imaging sensor.

17. The system of claim 16 , wherein the sample delivery component comprises at least two volumetric capillary tubes, a nozzle for mixing fluids within the at least two volumetric capillary tubes, and an output tip through which the fluid sample is delivered to the surface of the light sensitive imaging sensor.

18. The system of claim 13 , wherein the protruding element is at least partially elastic.

19. The system of claim 13 , wherein the body comprises a plate from which the protruding element extends.

20. The system of claim 19 , wherein a shape of the protruding element comprises a truncated pyramid having a larger base and a smaller base, the smaller base facing the light sensitive imaging sensor when the body is held by the carrier.

21. The system of claim 13 , wherein the device coupler is configured to transmit, from the mobile device to the light sensitive imaging sensor, electronic communications comprising an instruction to capture an image of a portion of the fluid sample placed on the surface to receive the fluid sample.

22. The system of claim 13 , comprising a second compartment configured to receive the mobile device.

23. The system of claim 13 , wherein the protruding element is at least partially transparent.

24. The system of claim 13 , wherein the body is not rigidly attached to the carrier.

Assignees

• Alentic Microscience Inc

Inventors

• Fine Alan Marc

Classifications

• B01L2300/0877

  Flow chambers · CPC title

• G02B21/361

  Optical details, e.g. image relay to the camera or image sensor (G02B21/364 takes precedence; illumination details G02B21/06 and subgroups) · CPC title

• G01N15/1484 Primary

  microstructural devices · CPC title

• G01N1/2813

  Producing thin layers of samples on a substrate, e.g. smearing, spinning-on (G01N1/30 takes precedence) · CPC title

• G01N2015/1486

  Counting the particles · CPC title