Lactobacillus fermentum bacteria with antifungal activity

Patent metadata

Field	Value
Publication number	US-11096396-B2
Application number	US-201816128460-A
Country	US
Kind code	B2
Filing date	Sep 11, 2018
Priority date	Aug 31, 2015
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

Disclosed is Bacterium of the species Lactobacillus rhamnosus CHCC15860 as deposited with the German Collection of Microorganisms and Cell Cultures (DSMZ) under accession No. DSM32092. Also disclosed are compositions comprising the bacterium, methods for producing fermented milk products using the bacterium and the products thus obtained.

First claim

The invention claimed is:

1. A method of producing a fermented milk product, comprising adding Lactobacillus rhamnosus of strain CHCC15860 deposited with the German Collection of Microorganisms and Cell Cultures (DSMZ) under accession number DSM32092 to milk or a milk product to obtain a mixture, and fermenting the mixture at a temperature between about 22° C. and about 43° C. until a pH of less than 4.6 is reached, wherein the Lactobacillus rhamnosus of strain CHCC15860 inhibits growth of one or more of the Penicillium solitum strain deposited with the DSMZ under accession number DSM32093 and the Penicillium brevicompactum strain deposited with the DSMZ under accession number DSM32094.

2. The method of claim 1 , wherein the method further comprises producing a food, feed or pharmaceutical product from the fermented milk product.

3. A food, feed or pharmaceutical product obtained by the method of claim 2 , wherein the method comprises:
adding the Lactobacillus rhamnosus of strain CHCC15860 to milk or a milk product to obtain a mixture;
fermenting the mixture at a temperature between about 22° C. and about 43° C. until a pH of less than 4.6 is reached to obtain a fermented milk product, and
producing a food, feed or pharmaceutical product from the fermented milk product,
wherein the Lactobacillus rhamnosus of strain CHCC15860 inhibits growth of one or more of the Penicillium satum strain deposited with the DSMZ under accession number DSM32093 and the Penicillium brevicompactum strain deposited with the DSMZ under accession number DSM32094.

4. A method of producing a fermented milk product, comprising adding a composition comprising Lactobacillus rhamnosus of strain CHCC15860 deposited with the German Collection of Microorganisms and Cell Cultures (DSMZ) under accession number DSM32092 to milk or a milk product to obtain a mixture, and fermenting the mixture at a temperature between about 22° C. and about 43° C. until a pH of less than 4.6 is reached, wherein the Lactobacillus rhamnosus of strain CHCC15860 inhibits growth of one or more of the Penicillium solitum strain deposited with the DSMZ under accession number DSM32093 and the Penicillium brevicompactum strain deposited with the DSMZ under accession number DSM32094.

5. The method of claim 4 , wherein the method further comprises producing a food, feed or pharmaceutical product from the fermented milk product.

6. A food, feed or pharmaceutical product obtained by the method of claim 5 , wherein the method comprises:
adding the composition comprising Lactobacillus rhamnosus of strain CHCC15860 to milk or a milk product to obtain a mixture;
fermenting the mixture at a temperature between about 22° C. and about 43° C. until a pH of less than 4.6 is reached to obtain a fermented milk product, and
producing a food, feed or pharmaceutical product from the fermented milk product,
wherein the Lactobacillus rhamnosus of strain CHCC15860 inhibits growth of one or more of the Penicillium sofitum strain deposited with the DSMZ under accession number DSM32093 and the Penicillium brevicompactum strain deposited with the DSMZ under accession number DSM32094.

7. The method of claim 4 , wherein the composition further comprises Lactobacillus fermentum of strain CHCC14591 deposited with the DSMZ under accession number DSM32086.

8. The method of claim 4 , wherein the composition further comprises a cryoprotective compound.

9. The method of claim 4 , wherein the composition is a solid frozen or freeze-dried starter culture comprising lactic acid bacteria in a concentration of at least 10 9 colony forming units per g (CFU/g) frozen material.

10. The method of claim 4 , wherein the composition is a solid frozen or freeze-dried starter culture comprising lactic acid bacteria in a concentration of at least 10 10 CFU/g frozen material.

11. The method of claim 4 , wherein the composition is a solid frozen or freeze-dried starter culture comprising lactic acid bacteria in a concentration of at least 10 11 CFU/g frozen material.

12. The method of claim 5 , wherein the composition is added in an amount to provide a concentration of the Lactobacillus rhamnosus of at least 10 7 CFU/g.

13. The method of claim 1 , further comprising adding Lactobacillus fermentum of strain CHCC14591 deposited with the DSMZ under accession number DSM32086 to the milk or milk product.

14. The method of claim 1 , wherein the Lactobacillus rhamnosus is added at a concentration of at least 10 9 CFU/g.

Assignees

• Chr Hansen As

Inventors

• Nielsen Cecilie Lykke Marvig
• Hornbaek Tina
• Rasmussen Pia
• Poulsen Lone
• Eckhardt Thomas
• Oeregaard Gunnar
• Moghadam Elahe Ghanei

Classifications

• A23B2/783

  Microorganisms; Enzymes · CPC title

• C12N1/205 Primary

  Bacterial isolates · CPC title

• A23C9/1234 Primary

  characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp. · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• A23K10/18

  of live microorganisms · CPC title