Apparatus and methods for detecting ATP in a liquid sample

Patent metadata

Field	Value
Publication number	US-11085063-B2
Application number	US-201916259234-A
Country	US
Kind code	B2
Filing date	Jan 28, 2019
Priority date	Jul 6, 2012
Publication date	Aug 10, 2021
Grant date	Aug 10, 2021

Abstract

Apparatuses and kits for the detection of ATP in a liquid sample are provided. The apparatuses and kits comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion comprises a fibrous or a foam material and is adapted to acquire and releasably retain a predetermined volume of a liquid sample or a sample of residue from a surface. The sampling device comprises a dry coating or a liquid coating, the coating including an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. Methods of use of the apparatus or kit are also provided.

First claim

What is claimed is:

1. An apparatus, comprising:
a container with an opening and a cuvette portion that is adapted to be operationally coupled to a luminometer;
a liquid reagent composition comprising luciferin, wherein the liquid reagent composition is disposed in a closed compartment, wherein the pH of the liquid reagent composition is about 6.8 or lower; and
a sampling device having a sampling portion;
wherein the sampling portion is adapted to acquire and releasably retain a sample;
wherein the sampling portion comprises a fibrous or a foam material;
wherein the sampling device comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with the liquid reagent composition, changes the pH of the liquid reagent composition to 6.9 or higher.

2. The apparatus of claim 1 , wherein the liquid reagent composition further comprises a luciferase enzyme.

3. The apparatus of claim 1 , wherein the sampling portion is adapted to obtain a predetermined volume of liquid.

4. The apparatus of claim 1 , wherein at least a portion of the coating is disposed on the sampling portion of the sampling device.

5. The apparatus of claim 1 , wherein the pH-adjusting reagent comprises a water-soluble reagent.

6. The apparatus of claim 1 , wherein the pH-adjusting reagent comprises a buffer component selected from the group consisting of N-[2-hydroxyethyl] piperazine-N′-[2-ethanesulfonic acid] (HEPES), N-[tris(hydroxymethyl)methyl]glycine (TRICINE), and combinations thereof.

7. The apparatus of claim 1 , wherein the coating further comprises an effective amount of a cell extractant.

8. The apparatus of claim 1 , wherein the liquid reagent composition further comprises a luciferase enzyme that comprises a recombinant luciferase enzyme.

9. The apparatus of claim 1 , wherein the liquid reagent composition further comprises a luciferase enzyme that consists essentially of a recombinant luciferase enzyme.

10. The apparatus of claim 1 , wherein the coating does not comprise an effective amount of a buffer component comprising phosphate.

11. The apparatus of claim 1 , wherein the sampling device is adapted to be contacted with the liquid reagent composition such that after contacting the sampling device with the liquid reagent composition a substantially steady state amount of light is emitted from the composition in less than 20 seconds.

Assignees

• 3M Innovative Properties Co

Inventors

• Driscoll Mark B
• Evans Rachel E
• Ramsay Catherine M
• Percy Neil

Classifications

• C12Q1/66

  involving luciferase · CPC title

• C12Q1/008 Primary

  for determining co-enzymes or co-factors, e.g. NAD, ATP · CPC title