Method for collecting biological material and device for collecting biological material

Patent metadata

Field	Value
Publication number	US-9988663-B2
Application number	US-201314434214-A
Country	US
Kind code	B2
Filing date	Oct 18, 2013
Priority date	Oct 19, 2012
Publication date	Jun 5, 2018
Grant date	Jun 5, 2018

Abstract

Provided is a device for collecting biological material from a microbe, the device including: a collection container that is used to collect a microbe from a liquid sample containing the microbe and to extract the biological material from the microbe; and a temperature control mechanism that controls a temperature of the collection container. Herein, the temperature control mechanism controls a temperature of the collection container so that the microbe is collected at a first temperature and the biological material is extracted from the microbe at a second temperature higher than the first temperature.

First claim

The invention claimed is:

1. A device for collecting ATP from a microbe, comprising:
a collection container that is used to collect a microbe from a medium containing the microbe and to extract the ATP from the microbe; and
a temperature control mechanism comprising a heating unit configured to heat the collection container, a temperature sensor configured to detect a temperature of the collection container and output temperature detection signals based on the temperature of the collection container, and a temperature controller having control logic configured to:
i) control the heating unit to heat the medium containing the microbe in the collection container to a predetermined first temperature set from 30° C. to less than 40° C. to collect the microbe from the medium at the first temperature while suppressing a decrease in bioactivity of the microbe based on the temperature detection signals outputted from the temperature sensor, and then keep the temperature at the first temperature to eliminate free ATP present outside the microbe based on the temperature detection signals outputted from the temperature sensor;
ii) raise the temperature of the collection container to a predetermined second temperature that is higher than the first temperature to extract ATP from the microbe at the second temperature based on the temperature detection signals outputted from the temperature sensor; and
iii) lower the temperature of the collection container back to the first temperature to collect a predetermined amount of ATP extract based on the temperature detection signals outputted from the temperature sensor.

2. The device for collecting ATP from a microbe according to claim 1 , wherein the temperature controller has further control logic configured to maintain the temperature of the collection container at the first temperature for a predetermined time while the predetermined amount of ATP extract is collected based on the temperature detection signals outputted from the temperature sensor.

Assignees

• Hitachi Plant Services Co Ltd
• Hitachi Ltd

Inventors

• Miyashita Noe
• Mori Shuichi
• Nakatsuka Yuta

Classifications

• C12M41/12

  of temperature (controlling the temperature of chemical or physical processes B01J19/0013, heating or cooling apparatus for laboratory use B01L7/00) · CPC title

• C12Q1/06 Primary

  Quantitative determination · CPC title

• C12M47/04

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• C12M33/00

  Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00) · CPC title

• C12Q1/008

  for determining co-enzymes or co-factors, e.g. NAD, ATP · CPC title