Biosensors for biological or chemical analysis and systems and methods for same

Patent metadata

Field	Value
Publication number	US-11080248-B2
Application number	US-201815995767-A
Country	US
Kind code	B2
Filing date	Jun 1, 2018
Priority date	Apr 16, 2012
Publication date	Aug 3, 2021
Grant date	Aug 3, 2021

Abstract

A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.

First claim

What is claimed is:

1. A biosensor comprising:
a detection device comprising a plurality of stacked layers and having a detector surface configured to support reaction sites, the stacked layers including a filter layer and a solid-state imager coupled to the filter layer, the filter layer comprising filter walls and a light-absorbing material that is deposited between adjacent filter walls, the light-absorbing material configured to prevent transmission of excitation light and permit transmission of emission signals, wherein the adjacent filter walls define a detection path therebetween through the corresponding light-absorbing material toward the solid-state imager, the filter walls configured to reflect the emission signals;
a coating covering at least a portion of the filter walls, the coating interposed between the filter wall and the light-absorbing material; and
a passivation layer positioned above the filter layer.

2. The biosensor of claim 1 , wherein the coating includes at least one of a reflective metal and a dielectric material.

3. The biosensor of claim 1 , wherein the coating has an index of refraction that is less than the index of refraction of the light-absorbing material.

4. The biosensor of claim 1 , wherein the coating extends entirely around the filter walls.

5. The biosensor of claim 4 , wherein the coating further extends between the light-absorbing material and the solid-state imager, such that the coating isolates the light-absorbing material from the filter walls and the solid-state imager.

6. The biosensor of claim 1 , wherein the thickness of the coating decreases as it extends towards the solid-state imager.

7. The biosensor of claim 1 , wherein the coated portion of the filter walls has a higher reflectivity than the non-coated portion of the filter wall.

8. The biosensor of claim 1 , wherein the coated portion of the filter walls is configured to provide less attenuation of the emission signals than the uncoated portion of the filter wall.

9. The biosensor of claim 1 , wherein the coated portion of the filter walls extends in a direction from the reaction sites toward the solid-state imager, and an uncoated portion of the filter walls extends in a direction from the first interior portion toward the solid-state imager.

10. The biosensor of claim 1 , wherein the detection path has a width that decreases as the detection path extends in a direction from the reaction sites toward the solid-state imager.

11. The biosensor of claim 1 , wherein the ratio between a height of the filter walls and a width of the detection path that extends between the adjacent filter walls is at least 2.5:1.

12. The biosensor of claim 1 , wherein the filter walls substantially define image areas on the detector surface, each reaction site being located within an image area and having a site area that is less than the image area.

13. The biosensor of claim 1 , the filter layer further comprising at least one of a dichroic filter layer and an array of microlenses.

14. The biosensor of claim 1 wherein a plurality of reaction chambers are defined by at least one of the passivation layer and the filter walls.

15. The biosensor of claim 1 wherein the passivation layer comprises at least one of silicon nitride (Si 3 N 4 ) and silica (SiO 2 ).

16. The biosensor of claim 1 wherein the passivation layer comprises a plurality of sub-layers.

17. The biosensor of claim 1 wherein:
the reaction sites are located on a top side of the biosensor that is opposite to a bottom side of the biosensor; and
the signal data from the light sensors is transmitted by one or more conductive vias to one or more electrical contacts located on the bottom side of the biosensor.

18. The biosensor of claim 1 , wherein the reaction sites include discrete metal regions on the detector surface having discrete clusters of nucleic acids.

19. The biosensor of claim 1 , wherein each of the light detectors has only a single pixel and corresponds to a single detection path.

20. The biosensor of claim 1 , further comprising a flow channel defined by the detector surface and a flow cell mounted to the detector surface, wherein at least a portion of the flow cell comprises a material that permits transmission of the excitation light.

21. A method of fabricating a biosensor, comprising:
providing a solid-state imager;
providing a substrate material onto the solid-state imager;
forming filter walls from the substrate material;
applying a coating to at least a portion of the filter walls;
depositing light-absorbing material into chambers defined by the filter walls;
applying a passivation layer onto the light-absorbing material; and
forming a detector surface with a plurality of reaction sites over the passivation layer.

Assignees

• Illumina Inc

Inventors

• Eltoukhy Helmy A
• Kain Robert C
• Feng Wenyi
• Pratt Mark
• Hirschbein Bernard
• Sabounchi Poorya
• Khurana Tarun

Classifications

• G01N33/6875

  Nucleoproteins · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G06F16/214 Primary

  Database migration support · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L2300/0654

  Lenses; Optical fibres · CPC title