Apparatus, system, and method for collecting a target material

Patent metadata

Field	Value
Publication number	US-11067487-B2
Application number	US-201715401054-A
Country	US
Kind code	B2
Filing date	Jan 8, 2017
Priority date	Nov 30, 2012
Publication date	Jul 20, 2021
Grant date	Jul 20, 2021

Abstract

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a plurality of processing vessels and a collector. The collector funnels portions of the target material from the suspension through a cannula and into the processing vessels. Sequential density fractionation is the division of a sample into fractions or of a fraction of a sample into sub-fractions by a step-wise or sequential process, such that each step or sequence results in the collection or separation of a different fraction or sub-fraction from the preceding and successive steps or sequences. In other words, sequential density fractionation provides individual sub-populations of a population or individual sub-sub-populations of a sub-population of a population through a series of steps.

First claim

We claim:

1. A system, comprising:
a primary vessel having an open end;
a device at least partially located within the open end of the primary vessel, the device comprising a cannula and a main body, wherein the main body is configured to form a seal with an inner wall of the primary vessel, and
a processing vessel comprising a plug in one end and an inner cavity comprising a displacement fluid having a density greater than at least a portion of buffy coat,
wherein the cannula extends through the plug of the processing vessel and accesses the inner cavity of the processing vessel,
wherein the device extends upwardly relative to the primary vessel, and
wherein the processing vessel extends upwardly from the device.

2. The system of claim 1 , further comprising:
a float having a density equivalent to the density of the buffy coat, the float to be inserted within the primary vessel.

3. The system of claim 2 , further comprising a clearing fluid having a density greater than the buffy coat to at least partially displace the buffy coat within the primary vessel.

4. The system of claim 2 , further comprising:
a sealing ring to tighten around the primary vessel to collapse the primary vessel inwardly until a seal is formed between the primary vessel and the float.

5. The system of claim 1 , the device further comprising a window within an inner wall of the main body to access at least a portion of a cavity.

6. The system of claim 1 , the device further comprising a shoulder extending circumferentially around the main body to inhibit further translation of the device into the primary vessel.

7. The system of claim 6 , further comprising a lock ring to apply to the outside of the primary vessel at the location of the shoulder to inhibit movement of the device relative to the primary vessel after the device is inserted into the primary vessel.

8. The system of claim 1 , further comprising a canopy to be inserted into a cavity to inhibit any fluid that is discharged through the cannula from escaping from an opening in a first end of the device.

9. The system of claim 1 , wherein the displacement fluid is a solution of colloidal silica particles coated with polyvinylpyrrolidone, a polysaccharide solution, iodixanol, an organic solvent, a liquid wax, an oil, a gas, olive oil, mineral oil, silicone oil, immersion oil, mineral oil, paraffin oil, silicon oil, fluorosilicone, perfluorodecalin, perfluoroperhydrophenanthrene, perfluorooctylbromide, organic solvents, 1,4-Dioxane, acetonitrile, ethyl acetate, tert-butanol, cyclohexanone, methylene chloride, tert-Amyl alcohol, tert-Butyl methyl ether, butyl acetate, hexanol, nitrobenzene, toluene, octanol, octane, propylene carbonate, tetramethylene sulfones, ionic liquids, a polymer-based solution, a surfactant, a perfluoroketone, perfluorocyclopentanone, perfluorocyclohexanone, a fluorinated ketone, a hydrofluoroether, a hydrofluorocarbon, a perfluorocarbon, a perfluoropolyether, silicon, a silicon-based liquid, phenylmethyl siloxane, or a combination thereof.

10. The system of claim 1 , wherein the plug is resealable.

11. The system of claim 1 , wherein the cannula is a needle.

12. A system for retrieving buffy coat from a blood sample, the system comprising:
a primary vessel, configured to retain the blood sample, having an open end;
a device comprising a cannula and a main body, the main body being at least partially located within the open end of the primary vessel, wherein the main body is configured to form a seal with an inner wall of the primary vessel; and
a processing vessel comprising a plug in one end and an inner cavity comprising a displacement fluid having a density greater than at least a portion of the buffy coat,
wherein the cannula extends through the plug of the processing vessel and accesses the inner cavity of the processing vessel,
wherein the device extends upwardly relative to the primary vessel, and
wherein the processing vessel extends upwardly from the device.

13. The system of claim 12 , further comprising:
a float having a density equivalent to the density of the buffy coat, the float to be inserted within the primary vessel.

14. The system of claim 13 , further comprising a clearing fluid having a density greater than the buffy coat to at least partially displace the buffy coat within the primary vessel.

15. The system of claim 13 , further comprising:
a sealing ring to tighten around the primary vessel to collapse the primary vessel inwardly until a seal is formed between the primary vessel and the float.

16. The system of claim 12 , the device further comprising a shoulder extending circumferentially around the main body to inhibit further translation of the device into the primary vessel, and
further comprising a lock ring to apply to the outside of the primary vessel at the location of the shoulder to inhibit movement of the device relative to the primary vessel after the device is inserted into the primary vessel.

17. The system of claim 12 , wherein the displacement fluid is a solution of colloidal silica particles coated with polyvinylpyrrolidone, a polysaccharide solution, iodixanol, an organic solvent, a liquid wax, an oil, a gas, olive oil, mineral oil, silicone oil, immersion oil, mineral oil, paraffin oil, silicon oil, fluorosilicone, perfluorodecalin, perfluoroperhydrophenanthrene, perfluorooctylbromide, organic solvents, 1,4-Dioxane, acetonitrile, ethyl acetate, tert-butanol, cyclohexanone, methylene chloride, tert-Amyl alcohol, tert-Butyl methyl ether, butyl acetate, hexanol, nitrobenzene, toluene, octanol, octane, propylene carbonate, tetramethylene sulfones, ionic liquids, a polymer-based solution, a surfactant, a perfluoroketone, perfluorocyclopentanone, perfluorocyclohexanone, a fluorinated ketone, a hydrofluoroether, a hydrofluorocarbon, a perfluorocarbon, a perfluoropolyether, silicon, a silicon-based liquid, phenylmethyl siloxane, or a combination thereof.

18. The system of claim 12 , wherein the plug is resealable.

19. The system of claim 12 , wherein the cannula is a needle.

20. The system of claim 12 , further comprising a clearing fluid having a density greater than the buffy coat to at least partially displace the buffy coat within the primary vessel.

Assignees

• Rarecyte Inc

Inventors

• Campton Daniel
• Nordberg Joshua
• Quarre Steve
• Seubert Ronald
• Lundt Jonathan
• U'Ren Lance

Classifications

• B01L3/5021 Primary

  Test tubes specially adapted for centrifugation purposes · CPC title

• B01L2300/0851

  Bottom walls · CPC title

• B01L2200/026

  Fluid interfacing between devices or objects, e.g. connectors, inlet details · CPC title

• B01L3/502

  with fluid transport, e.g. in multi-compartment structures · CPC title

• B01D21/262

  by using a centrifuge · CPC title