Apparatus, system, and method for collecting a target material

Patent metadata

Field	Value
Publication number	US-9945839-B2
Application number	US-201615351293-A
Country	US
Kind code	B2
Filing date	Nov 14, 2016
Priority date	Nov 30, 2012
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a displacement fluid, a collector, and a primary vessel. In another implementation, the system includes a processing vessel, a displacement fluid, a collector, and a primary vessel.

First claim

We claim:

1. A system comprising:
a primary vessel comprising an open end and a suspension comprising a target material; and
a collector comprising
a main body comprising a first end comprising a cavity and second end comprising an opening,
a flow regulator to control fluid flow, wherein the flow regulator extends from an apex of the opening to the cavity, and
a displacement fluid having a density greater than a density of at least a portion of the target material, wherein the displacement fluid is located within the cavity,

wherein at least the second end of the collector is located within the open end of the primary vessel, and
wherein the collector extends upwardly from the primary vessel.

2. The system of claim 1 , wherein the flow regulator is a valve.

3. The system of claim 2 , wherein the valve is closed when centrifugal forces are less than a predetermined amount and the valve is open when centrifugal forces are greater than or equal to a predetermined amount.

4. The system of claim 3 , wherein the predetermined amount is 2 g, 5 g, 10 g, 100 g, 1000 g, 2000 g, 2500 g, 3000 g, 5000 g, or 10000 g, and wherein g is the force of gravity.

5. The system of claim 2 , wherein the valve is a ball check valve, a diaphragm check valve, a swing check valve, a tilting disk check valve, a lift check valve, or a duckbill valve.

6. The system of claim 1 , wherein the flow regulator is a hole comprising a diameter sized and shaped to inhibit fluid flow between the cavity and the concave opening when not undergoing centrifugation.

7. The system of claim 6 , wherein the size of the diameter is based on the surface tensions of the displacement fluid and the target material.

8. The system of claim 1 , further comprising a float located at a longitudinal position within the primary vessel.

9. The system of claim 1 , further comprising a sealing ring located circumferentially around the primary vessel at the same longitudinal position as at least a portion of the float within the primary vessel.

10. The system of claim 1 , further comprising a seal between the second end of the collector and an inner wall of the primary vessel to maintain a fluid-tight sealing engagement between the end of the device and inner wall of the primary vessel.

11. The system of claim 1 , wherein the displacement fluid is selected from the group consisting of: a solution of colloidal silica particles coated with polyvinylpyrrolidone, a polysaccharide solution, iodixanol, a liquid wax, an oil, a gas, olive oil, mineral oil, silicone oil, immersion oil, mineral oil, paraffin oil, silicon oil, fluorosilicone, perfluorodecalin, perfluoroperhydrophenanthrene, perfluorooctylbromide, ionic liquids, a polymer-based solution, a surfactant, a perfluoroketone, perfluorocyclopentanone, perfluorocyclohexanone, a fluorinated ketone, a hydrofluoroether, a hydrofluorocarbon, a perfluorocarbon, a perfluoropolyether, silicon, a silicon-based liquid, phenylmethyl siloxane, and combinations thereof.

12. A system comprising:
a primary vessel comprising an open end and a suspension comprising a target material; and
a collector comprising
a main body comprising a first end comprising a cavity and second end comprising an opening,
a flow regulator to control fluid flow, wherein the flow regulator extends from an apex of the opening to the cavity,
a displacement fluid having a density greater than a density of at least a portion of the target material, wherein the displacement fluid is located within the cavity, and
a lid at or near the first end,

wherein at least the second end of the collector is located within the open end of the primary vessel.

13. A method comprising the steps of:
providing a primary vessel comprising an open end and a suspension comprising a target material;
inserting a collector into the open end of a primary vessel, the collector comprising:
a main body comprising a first end comprising a cavity and second end comprising an opening, wherein at least the second end is located within the open end of the primary vessel,
a flow regulator to control fluid flow, wherein the flow regulator extends from an apex of the opening to the cavity, and
wherein the collector extends upwardly from the primary vessel;

adding a displacement fluid to the cavity of the collector, the displacement fluid having a density greater than a density of at least a portion of the target material; and
centrifuging the primary vessel, the collector, and the displacement fluid, wherein the displacement fluid flows into the primary vessel via the flow regulator and displaces the at least one portion of the target material from the primary vessel into the cavity of the collector via the flow regulator.

14. The method of claim 13 , further comprising the step of: adding a float to the primary vessel prior to the separating step.

15. The method of claim 14 , further comprising the steps of:
separating the suspension into fractions by centrifugation after adding the float; and
removing at least one of the fractions from the primary vessel.

16. The method of claim 14 , further comprising the step of:
forming a seal between the float and the primary vessel after the separating step.

17. The method of claim 13 , wherein the flow regulator is a valve.

18. The method of claim 17 , wherein the valve is closed when centrifugal forces are less than a predetermined amount and the valve is open when centrifugal forces are greater than or equal to a predetermined amount.

19. The method of claim 13 , wherein the flow regulator is a hole comprising a diameter sized and shaped to inhibit fluid flow between the cavity and the concave opening when not undergoing centrifugation.

Assignees

• Rarecyte Inc

Inventors

• Campton Daniel
• Nordberg Joshua
• Quarre Steve
• Stewart David
• Seubert Ronald

Classifications

• B01D21/262

  by using a centrifuge · CPC title

• B01L2300/0851

  Bottom walls · CPC title

• G01N33/491 Primary

  by separating the blood components (G01N15/05 takes precedence) · CPC title

• G01N2001/4083

  sedimentation · CPC title

• B01L9/50

  Clamping means, e.g. tongs · CPC title