Automated smear making apparatus

Patent metadata

Field	Value
Publication number	US-11067485-B2
Application number	US-201816059447-A
Country	US
Kind code	B2
Filing date	Aug 9, 2018
Priority date	Dec 28, 2011
Publication date	Jul 20, 2021
Grant date	Jul 20, 2021

Abstract

An automated smear making apparatus used to prepare and smear samples on glass slides. In one embodiment, there is provided a smearing subsystem that generally includes a smear cartridge having: an input reel; at least one roll bar; a take-up reel; and a smearing tape. The smearing tape is initially wound within the input reel and coupled to the take-up reel such that the smearing tape can be drawn from the input reel and into the take-up reel. The smearing tape may include a plurality of perforations formed therein. The smearing tape may then be wrapped around the roll bar such that each of the plurality of perforations forms a blade that extends from the smearing tape to expose a smear surface as the smearing tape is drawn into the take-up reel. Alternatively, the smearing tape may be bent such that an edge of the smearing tape forms a smear surface between two roll bars. A slide transport surface is also provided to move a slide across the smear surface.

First claim

What is claimed is:

1. A smearing subsystem for use in an automated slide preparation apparatus, the smearing subsystem comprising:
a smear cartridge including
an input reel;
a take-up reel;
a smearing tape comprising a smear surface included on a blade of a cut-out of the smearing tape wherein the blade extends from the smearing tape to expose the smear surface as the smearing tape is drawn from the input reel and into the take-up reel;
a deflection component configured to deflect the smearing tape cut-out to create the blade; and
a slide transport surface configured to move a slide across the exposed smear surface.

2. The smearing subsystem of claim 1 , wherein the exposed smear surface of the blade forms a contact angle with the slide when the slide transport surface brings the slide in contact with the exposed smear surface such that the contact angle formed between the smear surface and the slide depends on a parameter of the sample.

3. The smearing subsystem of claim 2 , wherein the contact angle formed between the smear surface and the slide depends on the viscosity of the sample, the hematocrit level of the sample, or the volume of the sample.

4. The smearing subsystem of claim 1 , further comprising an identifier to identify the relative position of the smearing tape and an optical imaging subsystem.

5. The smearing subsystem of claim 4 , wherein the identifier comprises a plurality of index openings on the edge of the smearing tape.

6. The smearing subsystem of claim 4 , wherein the identifier comprises painted indicia on the smearing tape.

7. The smearing subsystem of claim 4 , wherein the optical imaging system comprises an optical sensor configured to sense the identifier on the smearing tape and set the position of the smearing tape on the optical imaging subsystem.

8. A method for automated slide preparation using a smear cartridge of a smearing subsystem, said method comprising the steps of:
moving a slide towards a smear surface of a smearing tape of the smear cartridge, wherein the smear surface is included on a blade of a cut-out of the smearing tape;
contacting the slide with the smear surface, wherein the smear surface is exposed when the position of the blade of the cut-out is extended by a deflection component of the smear cartridge as the smearing tape is drawn from the input reel and into the take-up reel; and
transporting the slide across the smear surface, wherein the exposed smear surface of the blade forms a contact angle with the slide when the slide transport surface brings the slide in contact with the exposed smear surface such that the contact angle formed between the smear surface and the slide depends on a parameter of the sample.

9. The method of claim 8 , wherein the contact angle formed between the smear surface and the slide depends on the viscosity of the sample, the hematocrit level of the sample, or the volume of the sample.

10. The method of claim 8 , wherein the smearing cartridge is configured such that the distance between the front of the deflection component and smear surface on the slide transport depends on the contact angle.

11. The method of claim 8 , wherein the smearing tape is initially wound within the input reel and coupled to the take-up reel.

12. The method of claim 8 , wherein the smearing tape comprises an identifier to identify the relative position of the smearing tape.

13. The method of claim 8 , further comprising an angle-control mechanism to adjust the contact angle formed between the exposed smear surface of the blade and the slide.

14. The method of claim 8 , wherein the angle-control mechanism is configured to adjust the position of:
i) of the deflection component with respect to the input reel;
ii) the deflection component with respect to the take-up reel;
iii) the input reel with respect to the deflection component; or
iv) the take-up reel with respect to the deflection component.

15. The method of claim 8 , wherein the contact angle is less than about 60 degrees, less than about 45 degrees, less than about 30 degrees, or less than about 15 degrees.

16. The method of claim 8 , wherein the deflection component comprises a roll bar.

Assignees

• Abbott Lab

Inventors

• Shoffner John Scott
• Chacko Koshy T
• Solomon Roei

Classifications

• B60T15/00

  Construction arrangement, or operation of valves incorporated in power brake systems and not covered by groups B60T11/00 or B60T13/00 (valve structures responsive to a speed condition B60T8/34; valves in general F16K) · CPC title

• B05C13/02

  for particular articles · CPC title

• G01N2035/00138

  Slides · CPC title

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title

• G01N1/2813 Primary

  Producing thin layers of samples on a substrate, e.g. smearing, spinning-on (G01N1/30 takes precedence) · CPC title