Method and an apparatus for an enzymatic hydrolysis, a liquid fraction and a solid fraction

Patent metadata

Field	Value
Publication number	US-11046983-B2
Application number	US-201716305924-A
Country	US
Kind code	B2
Filing date	Jun 1, 2017
Priority date	Jun 3, 2016
Publication date	Jun 29, 2021
Grant date	Jun 29, 2021

Abstract

In a method and an apparatus for an enzymatic hydrolysis in which plant based raw material is hydrolysed by means of enzymes in at least one enzymatic hydrolysis stage. A plant based feed ( 1 ) is fed to the enzymatic hydrolysis stage ( 2 ) in which the plant based feed is hydrolysed. A liquid fraction ( 3 ) comprising carbohydrates is separated from a solid fraction ( 4 ) in a solid-liquid separation stage ( 11 ). At least a part ( 5 ) of the solid fraction ( 4 ) comprising enzymes is recirculated to the plant based feed ( 1 ) of the enzymatic hydrolysis stage ( 2 ) or to the enzymatic hydrolysis stage ( 2 ), and a rest part ( 6 ) of the solid fraction ( 4 ) is recovered. Further, the invention relates to the liquid fraction and the solid fraction and their use.

First claim

The invention claimed is:

1. A method for an enzymatic hydrolysis in which wood based material is hydrolysed using enzymes in at least one enzymatic hydrolysis stage, the method comprising:
pretreating the wood based material using a combination of hydrolysis and steam explosion to form a wood based feed wherein the wood based feed comprises cellulose based material;
feeding the wood based feed to the enzymatic hydrolysis stage in which the wood based feed is hydrolysed;
separating a liquid fraction comprising carbohydrates from a solid fraction in a solid-liquid separation stage wherein most of the enzymes are attached to the solid fraction;
recirculating at least a part of the solid fraction comprising the enzymes to the wood based feed of the same or previous enzymatic hydrolysis stage; and
recovering a rest part of the solid fraction.

2. The method according to claim 1 , wherein the at least one enzymatic hydrolysis stage is more than one enzymatic hydrolysis stage, and the at least a part of the solid. fraction comprising enzymes is recirculated to the wood based feed of at least one of the enzymatic hydrolysis stages or to at least one of the enzymatic hydrolysis stages.

3. The method according to claim 1 , wherein the at least one enzymatic hydrolysis stage is more than one enzymatic hydrolysis stage, and the at least a part of the solid fraction comprising enzymes is recirculated back to the wood based feed of the same enzymatic hydrolysis stage or to the same enzymatic hydrolysis stage, and the rest part of the solid fraction is supplied to a next enzymatic hydrolysis stage.

4. The method according to claim 1 , wherein an average residence time of the at least one enzymatic hydrolysis stage is 4-72 hours.

5. The method according to claim I, wherein a consistency of the wood based feed is 4-40% by weight in the at least one enzymatic hydrolysis stage.

6. The method according to claim 1 , wherein the at least one enzymatic hydrolysis stage is more than one enzymatic hydrolysis stage, and a consistency of the wood based feed or the solid fraction is 10-40% by weight in a second or any later enzymatic hydrolysis stage.

7. The method according to claim 1 . further comprising:
diluting the rest part of the solid fraction with liquid; and
supplying the diluted rest part of the solid fraction to a next enzymatic hydrolysis stage or other treatment stage.

8. The method according claim 1 , wherein the liquid fraction is separated from the solid fraction using filtration, centrifugal treatment, or a combination thereof.

9. The method according to claim 1 , wherein the liquid fraction is recovered after the solid-liquid separation stage.

10. The method according to claim 1 , wherein a feed of the at least one enzymatic hydrolysis stage comprises the wood based feed of 20-60% by weight and the recirculated solid fraction of 40-80% by weight.

11. The method according to claim 1 , wherein the at least one enzymatic hydrolysis stage is more than one enzymatic hydrolysis stage, the method further comprising adding an enzyme in connection with a first enzymatic hydrolysis stage or a second or later enzymatic hydrolysis stage or the enzymatic hydrolysis stage to which the at least a part of the solid fraction is recirculated.

12. The method according to claim 1 , wherein the at least one enzymatic hydrolysis stage is more than one enzymatic hydrolysis stage, and a second or any later enzymatic hydrolysis stage is carried out without an enzyme addition.

13. The method according to claim 1 , wherein the wood based feed is thrilled from a wood based raw material, the wood based raw material being a wood based material or a mixture comprising a wood based material.

14. The method according to claim 1 , wherein the fraction is configured to be used as a source material in a fermentation, hydrolysis, chemical treatment, catalytic treatment, polymerization process, depolymerization process, degradation process, enzymatic treatment, manufacture of binder, manufacture of feed, manufacture of food, or any combination thereof.

15. The method according to claim 1 , wherein the solid fraction is configured to be used as a source material in a hydrolysis, polymerization process, depolymerization process, degradation process, chemical treatment, manufacture of a composite material, lignin composite, activated carbon, carbon fiber, binder material, polymers, resins, phenolic component, dispersion agent or absorbent material, manufacture of feed, manufacture of food, combustion process or other suitable process or their combinations.

Assignees

• Upm Kymmene Corp

Inventors

• Tamper Juha
• Turunen Sami

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12M1/40 Primary

  Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes · CPC title

• C13K1/02

  obtained by saccharification of cellulosic materials (manufacture of fodder A23K10/32) · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title