Apparatus for preparing immobilized-enzyme beads and method for preparing immobilized-enzyme beads using same

Patent metadata

Field	Value
Publication number	US-9777263-B2
Application number	US-201715599063-A
Country	US
Kind code	B2
Filing date	May 18, 2017
Priority date	May 17, 2012
Publication date	Oct 3, 2017
Grant date	Oct 3, 2017

Abstract

The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same.

First claim

The invention claimed is:

1. A method for producing a sweetener, comprising:
1) forming immobilized enzyme beads by introducing a mixed liquid containing an enzyme-containing material and an excipient into an immobilization apparatus, wherein the immobilization apparatus comprises:
a) a first tank into which the mixed liquid containing the enzyme-containing material and the excipient is injected;
b) a nozzle unit disposed at a lower end of the first tank and comprising a nozzle, the nozzle having an inner diameter of 0.1 mm to 1 mm and a cylindrical lower end and including a liquid outlet cut perpendicularly to a vertical axis of the nozzle at the lower end thereof; and
c) a second tank disposed below the nozzle unit and containing a calcium chloride solution; wherein the distance between the surface of the calcium chloride solution and the liquid outlet of the nozzle unit ranges from 0.1 m to 1.5 m;
2) adding the beads formed in step 1) to a reactor column;
3) transferring a solution containing a reactant for producing a sweetener to the reactor column;
4) producing the sweetener from the reactant by performing a reaction catalyzed by the enzyme.

2. The method according to claim 1 , wherein the nozzle unit in the immobilization apparatus according to claim 1 comprises at least two nozzles.

3. The method according to claim 1 , wherein the first tank in the immobilization apparatus comprises an air inlet.

4. The method according to claim 1 , wherein the excipient is alginate.

5. The method according to claim 1 , wherein the enzyme is an isomerase.

6. The method according to claim 1 , wherein the sweetener is tagatose.

7. The method according to claim 1 , wherein the reactant is galactose.

Assignees

• Cj Cheiljedang Corp
• Ch Cheiljedang Corp

Inventors

• Kim Soon Chul
• Kim Seong Bo
• Lim Jae Youn
• An Kwang Jin
• Kim Jin Ha

Classifications

• C12M21/18 Primary

  Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes · CPC title

• C12N11/14

  Enzymes or microbial cells immobilised on or in an inorganic carrier · CPC title

• C12M21/14 Primary

  for producing enzymes · CPC title

• C12M25/16

  Particles; Beads; Granular material; Encapsulation (chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00) · CPC title

• C12N11/00 Primary

  Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof · CPC title