Bioactive polypeptides for improvements in plant protection, growth and productivity

Patent metadata

Field	Value
Publication number	US-11046735-B2
Application number	US-202016929422-A
Country	US
Kind code	B2
Filing date	Jul 15, 2020
Priority date	Jul 20, 2017
Publication date	Jun 29, 2021
Grant date	Jun 29, 2021

Abstract

Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.

First claim

What is claimed is:

1. A composition for bioactive priming of a plant or a plant part to increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture, wherein the composition comprises:
(a) a root hair promoting polypeptide (RHPP) consisting of SEQ ID NO: 600;
and
(b) an agrochemical, a harpin or harpin-like polypeptide, a phytosulfokine (PSK) polypeptide, a flagellin or flagellin-associated polypeptide, or a combination of any thereof.

2. The composition of claim 1 , wherein the RHPP contains a chemical modification; is part of a fusion protein, wherein the fusion protein contains a protease recognition sequence; or a combination thereof.

3. The composition of claim 2 , wherein the chemical modification comprises acetylation, amidation, cyclization, PEGylation, or a combination of any thereof.

4. The composition of claim 2 , wherein the chemical modification comprises an N-terminal modification or a C-terminal modification.

5. The composition of claim 2 , wherein the chemical modification comprises N-terminal acetylation, C-terminal amidation, cross-linking, or cyclization.

6. The composition of claim 1 , wherein the RHPP is isolated, chemically synthesized, concentrated from a fermentation product, and/or partially purified.

7. The composition of claim 1 , wherein the RHPP is fused to a phloem targeting sequence to form a fused polypeptide, the amino acid sequence of the phloem targeting sequence comprising any one of SEQ ID NOs: 641-649, or any combination thereof, for delivering the fused polypeptide to vascular tissue or cells and/or phloem or phloem-associated tissue or cells in the plant or plant part.

8. The composition of claim 1 , wherein the composition comprises the flagellin or flagellin-associated polypeptide.

9. The composition of claim 1 , wherein an amino acid sequence of the flagellin or flagellin associated polypeptide comprises any one of SEQ ID NOs: 226-229, 232-234, 236-240, 243-246, 248, 250-256, 258, 259, 261, 263, 265-270, 272-280, 282, 283, 285, 286, 288-300, 526, 528, 532, 534, 536, 538, 540, 571-586, 752, 754-759, and 761-765.

10. The composition of claim 1 , wherein the composition comprises the harpin or harpin-like polypeptide.

11. The composition of claim 10 , wherein an amino acid sequence of the harpin or harpin-like polypeptide comprises SEQ ID NO: 587.

12. The composition of claim 1 , wherein the composition comprises the PSK polypeptide.

13. The composition of claim 1 , wherein the composition comprises the agrochemical, and the agrochemical comprises an antibiotic, a biopesticide, a preservative, a buffering agent, a wetting agent, a surfactant, a coating agent, a monosaccharide, a polysaccharide, an abrading agent, a pesticide, an insecticide, a herbicide, a nematicide, a bacteriocide, a fungicide, a miticide, a fertilizer, a biostimulant, an osmoprotectant, a colorant, a humectant, an amino acid, a biological control agent, or a combination of any thereof.

14. The composition of claim 13 , wherein the-agrochemical comprises a fungicide.

15. A seed coated with the composition of claim 1 .

16. The composition of claim 1 , wherein the composition comprises the RHPP and the flagellin or flagellin-associated peptide in a synergistically effective amount.

17. The composition of claim 14 , wherein the composition comprises the RHPP and the fungicide and the RHPP is present in an amount sufficient to reduce phytotoxicity of the fungicide.

18. The composition of claim 13 , wherein the composition comprises a surfactant.

19. The composition of claim 13 , wherein the composition comprises a preservative.

20. The composition of claim 6 , wherein the RHPP is purified by filtration, chromatography, or from a recombinant microorganism.

21. The composition of claim 1 , wherein the RHPP is present in the composition at a concentration of 0.00001% to 95%, based on the total weight of the composition.

22. The composition of claim 1 , wherein the composition comprises the agrochemical, and the agrochemical comprises boscalid, fluopyram, benodanil, benzovindflupyr, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxin, thifluzamide, fluxapyroxad, or a combination of any thereof.

23. The composition of claim 22 , wherein the agrochemical comprises boscalid, fluopyram, benzovindflupyr, fluxapyroxad, or a combination of any thereof.

24. The composition of claim 1 , wherein the composition comprises the agrochemical and the agrochemical comprises a strobilurin fungicide or a triazole fungicide.

25. A method for increasing growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decreasing abiotic stress in the plant or the plant part and/or protecting the plant or the plant part from disease, insects and/or nematodes, and/or increasing the innate immune response of the plant or the plant part and/or changing plant architecture, the method comprising either:
(a) applying the composition of claim 1 to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part to increase growth, yield, health, longevity, productivity, and/or vigor of the plant or the plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change the plant architecture; or
(b) applying the composition of claim 1 to a plant growth medium to increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part to be grown in the plant growth medium and/or decrease abiotic stress in the plant or the plant part to be grown in the plant growth medium and/or protect the plant or the plant part to be grown in the plant growth medium from disease, insects and/or nematodes, and/or increase the innate immune response and/or change plant architecture of the plant or the plant part to be grown in the plant growth medium.

Assignees

• Spogen Biotech Inc

Inventors

• Thompson Brian
• Leslie Michelle

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/82

  for plant cells {, e.g. plant artificial chromosomes (PACs)} · CPC title

• A61K38/16 Primary

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof {(enzyme inhibitors A61K38/005)} · CPC title

• A01N63/25

  Paenibacillus · CPC title

• A01N63/22

  Bacillus · CPC title