Method for enhancing resistance to pathogens in plants based on over-expression of impaired oomycete susceptibility 1

Patent metadata

Field	Value
Publication number	US-2015191742-A1
Application number	US-201414149266-A
Country	US
Kind code	A1
Filing date	Jan 7, 2014
Priority date	Jan 7, 2014
Publication date	Jul 9, 2015
Grant date	—

Abstract

The present invention provides a method for enhancing resistance to pathogens in plants, comprising over-expression of Arabidopsis thaliana Impaired Oomycete Susceptibility 1 (IOS1) or homologs thereof in plants. The present invention also provides transgenic plants with enhanced resistance to pathogens by over-expression of Arabidopsis thaliana Impaired Oomycete Susceptibility 1 (IOS1) or homologs thereof in the plants.

First claim

What is claimed is:

1 . A method for enhancing resistance to pathogens in plants, comprising over-expressing Arabidopsis thaliana Impaired Oomycete Susceptibility 1 (IOS1) or homologs thereof in the plants.

2 . The method of claim 1 , wherein an expression construct comprising the nucleotide sequence encoding IOS1 is transformed into the plants to produce transgenic plants over-expressing IOS1.

3 . The method of claim 2 , wherein the nucleotide sequence encoding IOS1 is SEQ ID NO: 1.

4 . The method of claim 1 , wherein the pathogens comprise Pseudomonas syringae pv. tomato DC3000, Pseudomonas syringae pv. syringae B728a and/or Botrytis cinerea.

5 . The method of claim 1 , wherein the plants comprise Solanaceae plants, Gramineous plants and/or Legumes.

6 . A transgenic plant over-expressing Arabidopsis thaliana Impaired Oomycete Susceptibility 1 (IOS1) or homologs thereof, which has enhanced resistance to pathogens.

7 . The transgenic plant of claim 6 , wherein the amino acid sequence of IOS1 is SEQ ID NO: 2.

8 . The transgenic plant of claim 6 , wherein after infection by pathogens, the transgenic plant comprises characteristics selected from the group consisting of: smaller infection area, increased callose (β-1,3 glucan) deposition, enhanced PTI marker genes such as FRK1 gene expression, higher MPK3 and MPK6 activities and inhibited pathogen-mediated stomata re-opening, when compared to the wild-type control plants.

9 . The transgenic plant of claim 6 , wherein the pathogens comprise Pseudomonas syringae pv. tomato DC3000, Pseudomonas syringae pv. syringae B728a and/or Botrytis cinerea.

10 . The transgenic plant of claim 6 , wherein the plant is selected from Solanaceae plants, Gramineous plants and Legumes.

Assignees

• Univ Nat Taiwan

Inventors

• Zimmerli Laurent
• Chen Ching-Wei
• Hsieh Yi-Ying

Classifications

• C12N15/8282 Primary

  for fungal resistance · CPC title

• C12N15/8281

  for bacterial resistance · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title