Method and reagent for quantifying cholesterol in high density lipoprotein 3

Patent metadata

Field	Value
Publication number	US-11041869-B2
Application number	US-201414906589-A
Country	US
Kind code	B2
Filing date	Jul 24, 2014
Priority date	Jul 24, 2013
Publication date	Jun 22, 2021
Grant date	Jun 22, 2021

Abstract

Disclosed are a method and a reagent for quantifying HDL3 in a test sample without requiring laborious operations. The method for quantifying cholesterol in high-density lipoprotein 3 comprises reacting a test sample with one or more surfactants which react specifically with high-density lipoprotein 3, and quantifying cholesterol. When one surfactant is used, the surfactant is one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers having an HLB of 12.5 to 15. When two or more surfactants are used, at least one of the surfactants is at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers, and the two or more surfactants are combined so as to provide the total HLB of 12.5 to 15 of the combined surfactants.

First claim

The invention claimed is:

1. A method for quantifying cholesterol in high-density lipoprotein 3, said method comprising:
reacting a test sample with two or more surfactants which react specifically with high-density lipoprotein 3; and
quantifying cholesterol;
wherein said two or more surfactants are ones selected from the group consisting of polyoxyethylene polycyclic phenyl ethers;
wherein a total concentration of said surfactants is 0.25 to 1.25 w/v % in terms of the final concentrations;
wherein the two or more surfactants provide a reaction ratio of high-density lipoprotein 3 to high-density lipoprotein 2 (HDL3/HDL2) of 4.6 to 10.6; and
wherein the two or more surfactants are combined so as to provide the total hydrophilic-lipophilic balance (HLB) of 13.7 to 14.0 of the combined surfactants.

2. The method according to claim 1 , wherein said polyoxyethylene polycyclic phenyl ethers are polyoxyethylene styrenated phenylethers.

3. The method according to claim 2 , wherein said polyoxyethylene styrenated phenylethers are polyoxyethylene distyrenated phenylethers.

4. The method according to claim 1 , wherein the total concentration of said surfactants is 0.5 to 1.25 w/v % in terms of the final concentrations.

5. The method according to claim 1 , wherein the total concentration of said surfactants is 0.25 to 1.0 w/v % in terms of the final concentrations.

Assignees

• Denka Company Ltd

Inventors

• Sato Noriyuki
• Itoh Yasuki

Classifications

• C12Q1/60 Primary

  involving cholesterol · CPC title

• G01N33/92 Primary

  involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} · CPC title

• G01N2405/00

  Assays, e.g. immunoassays or enzyme assays, involving lipids · CPC title

• C07C43/215

  having unsaturation outside the six-membered aromatic rings · CPC title

• C07C43/20

  having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring · CPC title