Method for isolating or detecting rare cell

Patent metadata

Field	Value
Publication number	US-11033900-B2
Application number	US-201615078463-A
Country	US
Kind code	B2
Filing date	Mar 23, 2016
Priority date	Mar 23, 2015
Publication date	Jun 15, 2021
Grant date	Jun 15, 2021

Abstract

A method for treating a blood specimen with which capturing rates for both a small rare cell and a rare cell having a high deformability can be improved in the case where rare cells are contained in a blood specimen. The method for isolating or detecting a rare cell includes treating a blood specimen using a filter to isolate or detect a rare cell in the blood specimen.

First claim

What is claimed is:

1. A method of isolating or detecting a rare cell having a high deformability, comprising filtering a blood specimen through a filter to isolate or detect a rare cell having a high deformability in the blood specimen,
wherein the filter includes slit shape holes having an average minor axis diameter of 5 μm or more and 8 μm or less and an average major axis diameter of 40 μm or more and 5000 μm or less at a hole density of 40 holes/mm 2 or more and 2000 holes/mm 2 or less with a ratio (w/z) between the average major axis diameter (w) and an average gap length (z) between the holes in a minor axis diameter direction being 7.0 or more and 130 or less,
wherein the filter is operated under filtration conditions such that the filter provides, with respect to the rare cell, a capturing rate of 80% or more.

2. The method according to claim 1 , wherein the blood specimen is filtered such that a filtering capacity per hole of the filter is 0.1 nl/μm 2 or more and 3 nl/μm 2 or less in terms of a treatment capacity per hole area of the filter.

3. The method according to claim 1 , wherein the filter has an opening rate of 10% or more to 60% or less.

4. The method according to claim 1 , wherein a filtration pressure during the filtering is 0.1 kPa or more and 2.6 kPa or less.

5. The method according to claim 1 , wherein a difference between pressures on an upper surface and a lower surface of the filter during the filtering is 100 Pa or less.

6. The method according to claim 1 , wherein the filtering comprises supplying the blood specimen to the filter at an average flow rate of 1 mm/min or more and 600 mm/min or less per hole of the filter.

7. The method according to claim 1 , wherein the rare cell is a cell selected from the group consisting of a cancer cell, a circulating tumor cell, a vascular endothelial cell, a vascular endothelial precursor cell, a cancer stem cell, an epithelial cell, a hematopoietic stem cell, a mesenchymal stem cell, a fetal cell, and combinations thereof.

8. A method of analyzing a rare cell in a blood specimen, comprising observing a kinetics, measuring an activity or analyzing a gene of the rare cell after the rare cell is isolated or detected according to the method of claim 1 .

9. The method according to claim 1 , wherein the filter has a hole density of 40 holes/mm 2 or more and 900 holes/mm 2 or less.

10. The method according to claim 1 , wherein the holes have an average major axis diameter of 40 μm or more and 500 μn or less.

11. The method according to claim 1 , wherein the ratio (w/z) is 7.3 or more and 129.1 or less.

12. The method according to claim 1 , wherein the holes have an average major axis diameter of 88 μm or more and 500 μn or less.

13. The method according to claim 1 , wherein the holes have an average gap length (z) of 7.5 μm or more and 12 μm or less.

Assignees

• Arkray Inc

Inventors

• Takagi Hidenori

Classifications

• G01N15/0625

  Optical scan of the deposits · CPC title

• B01D27/00

  Cartridge filters of the throw-away type · CPC title

• G01N2001/4088

  filtration · CPC title

• G01N1/4077

  by other techniques involving separation of suspended solids · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title