Methods and Compositions for Generating Bioactive Assemblies of Increased Complexity and Uses
US-2015374846-A1 · Dec 31, 2015 · US
Stable lignocellulolytic enzyme composition
US11028385B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11028385-B2 |
| Application number | US-201916353533-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 14, 2019 |
| Priority date | Mar 14, 2018 |
| Publication date | Jun 8, 2021 |
| Grant date | Jun 8, 2021 |
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- Title
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- Abstract
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Abstract
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The present invention relates to a formulation to stabilize lignocellulolytic enzyme mixture. This invention in particular relates to a formulation composed of lignocellulolytic enzyme with critical dosages of molasses either with petrochemical waste or glycerol as an additive to improve enzyme stability in a synergistic manner.
First claim
Opening claim text (preview).
What is claimed is: 1. A lignocellulolytic enzyme composition, said composition comprising: (a) 65% to 85% w/w lignocellulolytic enzyme mixture having a cellulase activity of 96 Filter Paper Unit (FPU)/ml, a β-glucosidase activity of about 850 β-glucosidase (BGL)/ml, a xylanase activity of about 1300 Units (U)/ml and a protein content of about 350 mg/ml; (b) 10% to 15% w/w additive; and (c) 5% to 20% w/w molasses; wherein the additive is a petrochemical waste stream comprising 0-0.5% w/w MEG (mono-ethylene glycol), 0.5-30% w/w DEG (di-ethylene glycol), 40-50% w/w TEG (tri-ethylene glycol), 25-50% w/w TTEG (tetra-ethylene glycol), 1.3% w/w ethylene glycols having 5-12 monomeric units, 5842 ppm of iron, 196 ppm sodium, 126 ppm chromium, 112 ppm boron, and 78 ppm of manganese. 2. The lignocellulolytic enzyme composition as claimed in claim 1 , wherein the molasses comprises at least 40% sugars. 3. The lignocellulolytic enzyme composition as claimed in claim 2 , wherein the sugar is selected from the group consisting of sucrose, glucose, and fructose.
Assignees
Inventors
Classifications
acting on glycosyl compounds (3.2) · CPC title
Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title
- C12N9/96Primary
Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title
hydrolysing O- and S- glycosyl compounds (3.2.1) · CPC title
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- What does patent US11028385B2 cover?
- The present invention relates to a formulation to stabilize lignocellulolytic enzyme mixture. This invention in particular relates to a formulation composed of lignocellulolytic enzyme with critical dosages of molasses either with petrochemical waste or glycerol as an additive to improve enzyme stability in a synergistic manner.
- Who is the assignee on this patent?
- Indian Oil Corp Ltd, Dept Of Biotechnology India
- What technology area does this patent fall under?
- Primary CPC classification C12N9/96. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 08 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).