Sporulation method of Bacillus bacterium

Patent metadata

Field	Value
Publication number	US-11008545-B2
Application number	US-201615565029-A
Country	US
Kind code	B2
Filing date	Apr 8, 2016
Priority date	Apr 9, 2015
Publication date	May 18, 2021
Grant date	May 18, 2021

Abstract

A method of producing Bacillus spores comprising a step of culturing the Bacillus bacterium using a liquid medium having a C/N ratio (weight ratio of carbon content to nitrogen content) of greater than 4 and less than 9.5.

First claim

The invention claimed is:

1. A method for increasing a spore production in Bacillus simplex , the method comprising culturing Bacillus simplex with a liquid medium having a C/N ratio (weight ratio of carbon content to nitrogen content) of from 4.5 to less than 9.5,
wherein a potassium content in the liquid medium is 1.9 g/L or less.

2. The method of claim 1 , wherein the C/N ratio in the liquid medium is from 4.5 to 7.5.

3. The method of claim 1 , wherein the C/N ratio in the liquid medium is from 6.0 to 7.5.

4. The method of claim 1 , wherein the carbon content in the liquid medium is 50 g/L or less.

5. The method of claim 1 , wherein the carbon content in the liquid medium is 25 g/L or less.

6. The method of claim 1 , wherein the carbon and nitrogen sources contained in the liquid medium are carbon and nitrogen sources which can be utilized by Bacillus simplex.

7. The method of claim 6 , wherein the carbon source which can be utilized by Bacillus simplex is one or more carbon sources selected from the group consisting of starch, glucose, lactose, glycerol, arabinose, ribose, xylose, galactose, fructose, mannose, inositol, mannitol, sorbitol, glucosamine, N-acetylglucosamine, cellobiose, maltose, sucrose, trehalose, xylitol, alcohols, organic acids, organic salts, and alkanes; and
the nitrogen source which can be utilized by Bacillus simplex is one or more nitrogen sources selected from the group consisting of soybean-derived components, yeast-derived components, corn-derived components, animal and plant proteins and hydrolysates thereof, and ammonium salts such as ammonium nitrate, ammonium sulfate, ammonium chloride, ammonium acetate, ammonia, sodium nitrate, potassium nitrate, sodium glutamate, urea and the like.

8. The method of claim 1 , wherein the liquid medium contains KH 2 PO 4 .

9. The method of claim 1 , wherein the liquid medium contains KH 2 PO 4 in an amount of from 0.5 to 5.5 g/L.

10. The method of claim 1 , wherein the liquid medium contains KH 2 PO 4 in an amount of from 0.5 to 2.0 g/L.

11. The method according to claim 1 , wherein the culturing is performed for 40 hours to 72 hours.

Assignees

• Idemitsu Kosan Co

Inventors

• Eguchi Takanori
• Morishita Yasuyuki
• Tsukagoshi Yuki

Classifications

• C12N3/00 Primary

  Spore forming or isolating processes · CPC title

• C12R2001/11

  Bacillus megaterium · CPC title

• C12R2001/07

  Bacillus · CPC title

• C12R2001/09

  Bacillus circulans · CPC title

• C12R2001/085

  Bacillus cereus · CPC title