Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-10995340-B2
Application number	US-201916374211-A
Country	US
Kind code	B2
Filing date	Apr 3, 2019
Priority date	Jan 14, 2010
Publication date	May 4, 2021
Grant date	May 4, 2021

Abstract

The invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants, and for specifying intracellular or extracellular localization of a gene product of interest. Transgenic plants, plant cells, plant parts, and seeds, comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided.

First claim

The invention claimed is:

1. A DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a sequence with at least 99 percent sequence identity to the full length of SEQ ID NO: 270 and having transcription termination activity; and
b) a sequence comprising SEQ ID NO: 270;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

2. The DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene of agronomic interest.

3. The DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene capable of providing herbicide resistance in plants.

4. The DNA molecule of claim 1 , wherein the transcribable polynucleotide molecule comprises a gene capable of providing plant pest control in plants.

5. A transgenic plant cell comprising a DNA construct comprising a sequence selected from the group consisting of:
a) a sequence with at least 99 percent sequence identity to the full length of SEQ ID NO: 270 and having transcription termination activity; and
b) a sequence comprising SEQ ID NO: 270;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

6. The transgenic plant cell of claim 5 , wherein said transgenic plant cell is a monocotyledonous plant cell.

7. The transgenic plant cell of claim 5 , wherein said transgenic plant cell is a dicotyledonous plant cell.

8. A transgenic plant, or part thereof, comprising the DNA molecule of claim 1 .

9. A progeny plant of the transgenic plant of claim 8 , or a part thereof, wherein the progeny plant or part thereof comprises said DNA molecule.

10. A transgenic seed, wherein the seed comprises the DNA molecule of claim 1 .

11. The DNA molecule of claim 1 , wherein the DNA molecule comprises a sequence with at least 99 percent sequence identity to the full length of SEQ ID NO: 270 having transcription termination activity.

12. The DNA molecule of claim 1 , wherein the DNA molecule comprises a sequence comprising SEQ ID NO: 270.

13. The transgenic plant cell of claim 5 , wherein the DNA construct comprises a sequence with at least 99 percent sequence identity to the full length of SEQ ID NO: 270 and having transcription termination activity.

14. The transgenic plant cell of claim 5 , wherein the DNA construct comprises a sequence comprising SEQ ID NO: 270.

Assignees

• Monsanto Technology Llc

Inventors

• Flasinski Stanislaw
• Dietrich Charles R
• Wu Wei
• Li Zhaolong
• Qiu Bo-Xing
• Guo Liang
• Chittoor Jaishree M

Classifications

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8222 Primary

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title