Nanoliter array loading

Patent metadata

Field	Value
Publication number	US-10974247-B2
Application number	US-201816119327-A
Country	US
Kind code	B2
Filing date	Aug 31, 2018
Priority date	Mar 12, 2004
Publication date	Apr 13, 2021
Grant date	Apr 13, 2021

Abstract

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

First claim

What is claimed is:

1. A method of processing a fluid for use in a microfluidic sample system, the method comprising:
providing a perfluorinated liquid including dissolved gases; and
removing the dissolved gases from the perfluorinated liquid so as to provide a degassed perfluorinated liquids wherein removing the dissolved gases includes subjecting the perfluorinated liquid to a vacuum environment; and
wherein providing a perfluorinated liquid includes immersing in the perfluorinated liquid a plurality of hollow pipettes having an open end and a closed end, and wherein the vacuum environment causes the pipettes to collapse, the method further comprising:
repressurizing the perfluorinated liquid back to atmospheric pressure such that the pipettes reinflate so as to draw the degassed perfluorinated liquid into the pipettes.

2. A method of processing a fluid for use in a microfluidic sample system, the method comprising:
providing a perfluorinated liquid including dissolved gases;
immersing in the perfluorinated liquid a plurality of hollow pipettes having an open end and a closed end; and
removing the dissolved gases from the perfluorinated liquid so as to provide a degassed perfluorinated liquid.

3. A method according to claim 2 , wherein removing the dissolved gases includes subjecting the perfluorinated liquid to a vacuum environment.

4. A method according to claim 3 , wherein the vacuum environment causes the pipettes to collapse, the method further comprising:
repressurizing the perfluorinated liquid back to atmospheric pressure such that the pipettes reinflate so as to draw the degassed perfluorinated liquid into the pipettes.

5. A method according to claim 4 , further comprising:
sealing the open ends of the filled pipettes.

6. A method according to claim 5 , further comprising:
sealing the filled pipettes in a vacuum-packed bag.

7. A method of processing a fluid for use in a microfluidic sample system, the method comprising:
providing a perfluorinated liquid including dissolved gases; and
removing the dissolved gases from the perfluorinated liquid so as to provide a degassed perfluorinated liquid; and
at least partially filling a plate case with the perfluorinated liquid.

8. A method according to claim 7 , at least partially filling a volume of the plate case with the perfluorinated liquid the volume including a plate configured to receive a liquid sample and to enclose the liquid sample in the perfluorinated liquid.

9. A method according to claim 8 , further comprising loading the plate with the liquid sample.

10. A method according to claim 8 , wherein the plate comprises a plurality of wells, the method further comprising loading one or more of the sample wells with the liquid sample.

11. A method according to claim 10 , wherein one or more of the sample wells comprises a through-hole sample well.

12. A method according to claim 9 , wherein the liquid sample comprises one or more of nucleic acids, cells, or proteins.

13. A method according to claim 9 , further comprising performing PCR on the sample within the plate.

14. A method according to claim 9 , further comprising thermal cycling the sample within the plate.

Assignees

• Life Technologies Corp

Inventors

• Kanigan Tanya S
• Smith Steve
• Linton John
• Hess Robert
• Yoder Karl
• Brenan Colin J H

Classifications

• B01L3/0244 Primary

  using pins · CPC title

• Y10T436/2525

  Stabilizing or preserving · CPC title

• B01L3/5025

  for parallel transport of multiple samples · CPC title

• B01L2200/027

  for microfluidic devices · CPC title

• Y10T137/1624

  Destructible or deformable element controlled · CPC title