Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof

Patent metadata

Field	Value
Publication number	US-10973229-B2
Application number	US-201916434995-A
Country	US
Kind code	B2
Filing date	Jun 7, 2019
Priority date	Dec 3, 2010
Publication date	Apr 13, 2021
Grant date	Apr 13, 2021

Abstract

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

First claim

The invention claimed is:

1. A method of protecting a soybean plant from weeds wherein the soybean plant comprises SEQ ID NO:27, said method comprising applying a herbicide selected from the group consisting of a phenoxyacetic acid herbicide, a glyphosate herbicide, and a glufosinate herbicide to a field, said field comprising the soybean plant comprising SEQ ID NO:27.

2. The method of claim 1 , wherein the herbicide is a phenoxyacetic acid herbicide.

3. The method of claim 2 , wherein the phenoxyacetic acid herbicide is 2,4-D.

4. The method of claim 1 , wherein said method comprises applying at least two of said herbicides simultaneously.

5. The method of claim 1 , wherein said method comprises applying at least two of said herbicides sequentially.

6. The method of claim 1 , wherein said phenoxyacetic acid herbicide is selected from the group consisting of 2,4-D and 2,4-DB.

7. The method of claim 1 , wherein said herbicide is applied over the top of said plant.

Assignees

• Dow Agrosciences Llc
• Ms Technologies Llc

Inventors

• Cui Yunxing C
• Hoffman Thomas
• Zhou Ning
• Novak Stephen N
• Colon Julissa
• Parkhurst Dawn M
• Toledo Sandra G
• Wright Terry R
• Russell Sean M
• Held Bruce
• Sekar Vaithilingam

Classifications

• A01N39/04 Primary

  Aryloxy-acetic acids; Derivatives thereof · CPC title

• C12N9/93 Primary

  Ligases (6) · CPC title

• C12N9/1092 Primary

  3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• C12Y604/01002

  Acetyl-CoA carboxylase (6.4.1.2) · CPC title