Process for treating arabinoxylan-containing substrate

Patent metadata

Field	Value
Publication number	US-10954499-B2
Application number	US-201916281926-A
Country	US
Kind code	B2
Filing date	Feb 21, 2019
Priority date	Apr 26, 2005
Publication date	Mar 23, 2021
Grant date	Mar 23, 2021

Abstract

The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

First claim

The invention claimed is:

1. An industrial process for treating an arabinoxylan-containing substrate, comprising:
contacting the arabinoxylan-containing substrate with a polypeptide having arabinofuranosidase activity, wherein the polypeptide having arabinofuranosidase activity is a polypeptide with at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4 with modifications selected from the group consisting of:
deletions of 1 to 30 amino acid residues;
insertions of up to 25 amino acid residues;
conservative substitutions of basic, acidic, polar, hydrophobic, aromatic and small amino acids, wherein the small amino acids are selected from the group consisting of:
glycine, alanine, serine, threonine and methionine; and combinations thereof.

2. The process of claim 1 , wherein the polypeptide has at least 96% sequence identity to the mature polypeptide of SEQ ID NO: 4.

3. The process of claim 1 , wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 4.

4. The process of claim 1 , wherein the polypeptide has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 4.

5. The process of claim 1 , wherein the polypeptide has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 4.

6. The process of claim 1 , wherein the polypeptide comprises the mature polypeptide of SEQ ID NO: 4.

7. The process of claim 1 , wherein the polypeptide is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with the full-length complement of the nucleic acid sequence of nucleotides 46 to 1974 of SEQ ID NO: 3; wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA, and 50% formamide for high stringency, following standard Southern blotting procedures, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.

8. The process of claim 1 , wherein the polypeptide is encoded by a nucleic acid sequence which hybridizes under very high stringency conditions with the full-length complement of the nucleic acid sequence of nucleotides 46 to 1974 of SEQ ID NO: 3; wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA, and 50% formamide for high stringency, following standard Southern blotting procedures, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.

9. The process of claim 1 , wherein the polypeptide has a deletion from the amino and/or carboxyl terminus of the mature polypeptide of SEQ ID NO: 4 and has at least 430 amino acids of the mature polypeptide of SEQ ID NO: 4.

10. The process of claim 1 , wherein the process is for producing fuel ethanol from cellulose containing biomass.

11. The process of claim 1 , wherein the process is for producing fuel and/or potable ethanol from starch.

12. The process of claim 1 , wherein the process is for mashing in beer production.

13. The process of claim 1 , wherein the process is for dough making.

14. The process of claim 1 , wherein the process is for manufacturing an animal feed product.

Assignees

• Novozymes As

Inventors

• Soerensen Hanne Risbjerg
• Joergensen Christel Thea
• Christensen Lars Hylling
• Joergensen Christian Isak
• Hansen Carsten Hoerslev
• Kofod Lene Venke

Classifications

• C12Y302/01055

  Alpha-N-arabinofuranosidase (3.2.1.55) · CPC title

• A23K20/168

  Steroids · CPC title

• A21D8/042

  with enzymes · CPC title

• C12N9/2402 Primary

  hydrolysing O- and S- glycosyl compounds (3.2.1) · CPC title

• A23L29/06

  Enzymes · CPC title