Process for treating an arabinoxylan-containing substrate

Patent metadata

Field	Value
Publication number	US-10233434-B2
Application number	US-201715725660-A
Country	US
Kind code	B2
Filing date	Oct 5, 2017
Priority date	Apr 26, 2005
Publication date	Mar 19, 2019
Grant date	Mar 19, 2019

Abstract

The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

First claim

The invention claimed is:

1. A process for treating an arabinoxylan-containing substrate, comprising contacting the arabinoxylan-containing substrate with a polypeptide having arabinofuranosidase activity, wherein the polypeptide having arabinofuranosidase activity is selected from the group consisting of:
(a) a polypeptide with at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2; and
(b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with the full-length complement of the nucleic acid sequence of nucleotides 55 to 1677 of SEQ ID NO: 1;
wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA, and 50% formamide for high stringency, following standard Southern blotting procedures, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.

2. The process of claim 1 , wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

3. The process of claim 1 , wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

4. The process of claim 1 , wherein the polypeptide comprises the mature polypeptide of SEQ ID NO: 2.

5. The process of claim 1 , wherein the polypeptide is encoded by a nucleic acid sequence which hybridizes under very high stringency conditions with the full-length complement of the nucleic acid sequence of nucleotides 55 to 1677 of SEQ ID NO: 1; wherein the very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA, and 50% formamide for high stringency, following standard Southern blotting procedures, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.

6. The process of claim 1 , wherein the polypeptide is a fragment of the mature polypeptide of SEQ ID NO: 2 and has arabinofuranosidase (EC 3.2.1.55) activity, wherein the fragment has one or more amino acids deleted from the amino and/or carboxyl terminus of the mature polypeptide of SEQ ID NO: 2 and has at least 430 amino acids of the mature polypeptide of SEQ ID NO: 2.

7. The process of claim 1 , wherein the polypeptide comprises the mature polypeptide of SEQ ID NO: 2 and exhibits arabinofuranosidase activity.

8. The process of claim 1 , wherein the process is producing fuel ethanol from cellulose containing biomass, producing fuel and/or potable ethanol from starch, mashing for beer production, in a dough making, or manufacturing an animal feed product.

Assignees

• Novozymes As

Inventors

• Soerensen Hanne Risbjerg
• Joergensen Christel Thea
• Christensen Lars Hylling
• Joergensen Christian Isak
• Hansen Carsten Hoerslev
• Kofod Lene Venke

Classifications

• A21D8/042

  with enzymes · CPC title

• C12N9/2402 Primary

  hydrolysing O- and S- glycosyl compounds (3.2.1) · CPC title

• A23K20/168

  Steroids · CPC title

• C12Y302/01055

  Alpha-N-arabinofuranosidase (3.2.1.55) · CPC title

• A23L29/06

  Enzymes · CPC title