Fluorescence imaging apparatus

Patent metadata

Field	Value
Publication number	US-10952616-B2
Application number	US-201916368510-A
Country	US
Kind code	B2
Filing date	Mar 28, 2019
Priority date	Mar 30, 2018
Publication date	Mar 23, 2021
Grant date	Mar 23, 2021

Abstract

A method of fluorescence calibration. An excitation light is provided and incident on an object through an optical probe. The fluorescence light generated from the object is detected. Structural data of the object is acquired, and an optical attenuation property of the object is calculated based on the structural data. The fluorescence intensity is then calibrated based on the optical attenuation property.

First claim

The invention claimed is:

1. A method of fluorescence calibration, comprising:
providing an excitation light incident on an object through an optical probe;
detecting a fluorescence light generated from the object;
acquiring structural data of the object;
calculating an optical attenuation property of the object based on the structural data; and
calibrating fluorescence intensity based on the optical attenuation property and a depth calibration factor.

2. The method according to claim 1 , further comprising calibrating the fluorescence intensity based on a distance between the optical probe and the object.

3. The method according to claim 1 , further comprising calculating a distance between the optical probe and the object.

4. The method according to claim 1 , further comprising detecting the fluorescence light and acquiring the structural data simultaneously.

5. The method according to claim 1 , wherein the fluorescence light and the structural data are delivered by a double clad fiber.

6. The method according to claim 5 , wherein the structural data is delivered by a core of the double clad fiber, and the fluorescence light is delivered by a cladding of the double clad fiber.

7. The method according to claim 1 , further comprising acquiring the structural data by optical coherence tomography.

8. The method according to claim 1 , wherein the optical attenuation property includes attenuation coefficient of an OCT image.

9. The method according to claim 1 , further comprising applying the calibrated fluorescence intensity for correcting a fluorescence image constructed by the fluorescence light.

10. A method of fluorescence calibration, comprising:
providing an excitation light incident on an object through an optical probe;
detecting fluorescence intensity of a fluorescence light generated from the object;
acquiring structural data of the object;
calculating a distance between the optical probe and the object;
calibrating the fluorescence intensity based on the distance;
wherein if the fluorescence intensity calibrated based on the distance is higher than a predetermined level, then calculating an optical attenuation property of the object is based on the structural data; and
wherein if the fluorescence intensity calibrated based on the distance is higher than the predetermined level, then calibrating the fluorescence intensity of the fluorescence light based on the optical attenuation property and a depth calibration factor.

11. The method of claim 10 , further comprising detecting the fluorescence light and obtaining the structural data simultaneously.

12. The method according to claim 10 , wherein the fluorescence light and the structural data are delivered by a double clad fiber.

13. The method according to claim 10 , wherein the structural data is delivered by a core of the double clad fiber, and the fluorescence light is delivered by a cladding of the double clad fiber.

14. The method according to claim 10 , further comprising acquiring the structural data by optical coherence tomography (OCT).

15. The method according to claim 10 , wherein the optical attenuation property includes attenuation coefficient of an OCT image.

16. The method according to claim 10 , further comprising applying only the fluorescence intensity calibrated by a first calibration factor for correcting a fluorescence image constructed by the fluorescence light if the fluorescence intensity calibrated by the first calibration factor is not higher than the predetermined level.

17. The method according to claim 10 , further comprising applying the fluorescence intensity calibrated by both a first calibration factor and a second calibration factor for correcting a fluorescence image constructed by the fluorescence light if the fluorescence intensity calibrated by the first calibration factor is higher than the predetermined level.

Assignees

• Canon Usa Inc

Inventors

• Watanabe Kohei

Classifications

• G01N21/4795

  spatially resolved investigating of object in scattering medium (in vivo A61B) · CPC title

• A61B5/0066 Primary

  Optical coherence imaging · CPC title

• G01N21/6458 Primary

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• A61B5/0075

  by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy (A61B5/0071 takes precedence) · CPC title

• G01N21/274

  Calibration, base line adjustment, drift correction · CPC title