Spinning apparatus for measurement of characteristics relating to molecules

Patent metadata

Field	Value
Publication number	US-10948401-B2
Application number	US-201716079995-A
Country	US
Kind code	B2
Filing date	Feb 24, 2017
Priority date	Feb 25, 2016
Publication date	Mar 16, 2021
Grant date	Mar 16, 2021

Abstract

An apparatus for measuring a characteristic of a sample using a centrifuge and optical components is disclosed. The centrifuge may be a standard benchtop centrifuge. The optical components may be sized and dimensioned to fit, along with the sample, inside the centrifuge.

First claim

What is claimed is:

1. An apparatus for measuring a characteristic of a sample, the apparatus being a spinning force system, and comprising:
a module comprising:
a sample holder;
a light source configured to illuminate the sample;
an imaging axis oriented along a direction at which light from the light source hits the sample holder; and
a detector configured to receive light from the light source,

wherein the module is sized and dimensioned to fit within a centrifuge receptacle having a volume of less than or equal to 1 L,
and wherein the spinning force system is arranged such that a direction of centrifugal force applied to the sample holder by a centrifuge and the imaging axis are misaligned in order to track lateral particle motion.

2. The apparatus of claim 1 , wherein the volume is of less than or equal to 400 mL.

3. The apparatus of claim 1 , wherein the sample holder, light source and detector are physically misaligned.

4. The apparatus of claim 3 , wherein module further comprises at least one mirror that directs light from the light source to the sample holder or to the detector.

5. The apparatus of claim 3 , wherein the sample holder and light source are aligned along a line and the detector is misaligned from the line.

6. The apparatus of claim 5 , wherein the module forms a U-shape having a first leg and a second leg.

7. The apparatus of claim 6 , wherein the sample holder and light source are positioned within the first leg and the detector is positioned within the second leg.

8. The apparatus of claim 6 , wherein the first leg is longer than the second leg.

9. The apparatus of claim 1 , wherein the module further comprises an objective.

10. The apparatus of claim 9 , wherein the sample holder, light source and objective are aligned along a line and the detector is misaligned from the line.

11. A method comprising:
attaching a particle to a surface through a molecular interaction associated with a first molecule and a second molecule;
rotating the surface about an axis of rotation to apply a centrifugal force to the particle, the centrifugal force having a direction;
hitting the particle with light from a light source;
detecting an image of the particle with a detector during rotation of the surface, the image containing information representing a characteristic of the molecular interaction; and
determining the characteristic of the molecular interaction based on the detected image, wherein an imaging axis is angled relative to the direction of the centrifugal force, the imaging axis being oriented along the direction at which light from the light source hits the particle to permit tracking of lateral particle motion.

12. The method of claim 11 , wherein the surface is held within a centrifugal bucket, and the bucket is at an oblique angle relative to the axis of rotation.

13. The method of claim 11 , wherein:
the step of attaching a particle to a surface comprises attaching a nucleic acid nanoswitch to the surface, wherein the nucleic acid nanoswitch comprises a nucleic acid conjugated to the particle on its free end.

14. The method of claim 11 , further comprising:
inserting the surface into a centrifuge; and
selecting a centrifuge temperature using a temperature control built into the centrifuge.

15. The method of claim 14 , further comprising:
changing the centrifuge temperature using the temperature control built into the centrifuge.

16. The method of claim 11 , wherein:
the step of rotating the surface about the axis of rotation comprises rotating the surface a first time; after rotating the surface the first time, stopping rotation of the surface; and after stopping rotation of the surface, rotating the surface a second time to apply a force to the particle;
the step of detecting an image of the particle comprises detecting images of the particle during rotation of the surface the first time and the second time, the images containing information representing a characteristic of the molecular interaction; and
the step of determining the characteristic of the molecular interaction comprises determining the characteristic of the molecular interaction based on the detected images.

17. The apparatus of claim 1 , further comprising the centrifuge.

Assignees

• Childrens Medical Center
• Harvard College

Inventors

• Yang Darren
• Ward Andrew
• Wong Wesley Philip
• Halvorsen Kenneth Anders

Classifications

• G01N15/1434 Primary

  Optical arrangements · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• G01N2015/0038

  Investigating nanoparticles · CPC title

• C12Q1/68

  involving nucleic acids · CPC title

• G01N2203/0089

  Biorheological properties · CPC title