Host-guest metal organic framework systems

Patent metadata

Field	Value
Publication number	US-10947321-B2
Application number	US-201515322976-A
Country	US
Kind code	B2
Filing date	May 19, 2015
Priority date	Jul 3, 2014
Publication date	Mar 16, 2021
Grant date	Mar 16, 2021

Abstract

A method for producing Metal Organic Framework (MOF) having a framework that encapsulates a bio-molecule, the method comprising combining in a solution the bio-molecule and MOF precursors, wherein the bio-molecule promotes formation of the encapsulating framework. The method stems from a surprising effect that a bio-molecule can promote or trigger the formation of MOF when combined together in a solution with MOF precursors. That is, it has now been found that a bio-molecule can effectively act as a seed around which the framework forms, with the resulting framework encapsulating the bio-molecule.

First claim

The claims defining the invention are as follows:

1. A method for producing Metal Organic Framework (MOF) having a framework that encapsulates a bio-molecule selected from the group consisting of a protein, a peptide, a nucleic acid, an amino acid, and a combination thereof, the bio-molecule having a smallest dimension that is at least 1.5 times larger than the largest cavity diameter (LCD) of any intrinsic cavity of the framework, and the method comprising combining in a particular order the bio-molecule and MOF precursors that comprise an organic ligand and a metal salt under conditions selected to form a framework that encapsulates said bio-molecule, the particular order being:
(i) combining a solution comprising both the bio-molecule and the organic ligand with a separate solution comprising the metal salt, or
(ii) combining together at the same time three solutions each separately comprising the metal salt, the organic ligand, and the bio-molecule, respectively,
wherein the encapsulating framework is formed around said bio-molecule at a solution temperature that is lower than 75° C., such that the bio-molecule sits within the MOF framework as a bioactive, heterogeneous and discontinuous guest phase within a self-defined cavity.

2. The method according to claim 1 , wherein the bio-molecule is a protein which is an enzyme.

3. The method according to claim 1 , wherein the bio-molecule is a protein and has a concentration in the solution of between 0.1 and 20 mg/mL.

4. The method according to claim 1 , wherein the bio-molecule is an amino acid and has a concentration in the solution of between 0.1 and 100 mg/mL.

5. The method according to claim 1 , wherein the bio-molecule is a nucleic acid and has a concentration in the solution of between 0.001 and 100 μM.

6. The method according to claim 1 , wherein the MOF precursors have a concentration in the solution of between about 0.001 M and 1 M.

7. The method according to claim 1 , wherein the MOF encapsulates from 1 wt % to 32 wt % bio-molecule relative to the weight of the MOF.

8. A method of producing crystalline Metal Organic Framework (MOF) having a framework that defines intrinsic cavities and encapsulates a bio-molecule, said method comprising:
a) combining in a particular order the bio-molecule, and MOF precursors that comprise an organic ligand and a metal salt, the particular order being:
(i) combining a solution comprising both the bio-molecule and the organic ligand with a separate solution comprising the metal salt, or,
(ii) combining together at the same time three solutions each separately comprising the metal salt, the organic ligand, and the bio-molecule, respectively, and

b) forming the encapsulating framework around said bio-molecule at a solution temperature that is lower than 75° C.,
wherein the bio-molecule (i) promotes formation of the encapsulating framework, (ii) has a smallest dimension that is at least 1.5 times larger than the largest cavity diameter (LCD) of any intrinsic cavity of the framework, and (iii) sits within the MOF framework as a bioactive, heterogeneous and discontinuous guest phase within a self-defined cavity.

9. The method according to claim 8 , wherein the LCD is between 5 Å and 500 Å.

10. The method according to claim 8 , wherein the bio-molecule is a protein, a peptide, a nucleic acid, an amino acid, or a combination thereof.

11. A method for producing Metal Organic Framework (MOF) having a framework that encapsulates a bio-molecule, the method comprising combining in a particular order in a solution the bio-molecule and MOF precursors that comprise an organic ligand and a metal salt, the particular order being:
(i) combining a solution comprising both the bio-molecule and the organic ligand with a separate solution comprising the metal salt, or
(ii) combining together at the same time three solutions each separately comprising the metal salt, the organic ligand, and the bio-molecule, respectively,
wherein
the bio-molecule promotes formation of the encapsulating framework, and
the bio-molecule has a smallest dimension that is at least 1.5 times larger than the largest cavity diameter (LCD) of any intrinsic cavity of the framework; and
the encapsulating framework is formed around said bio-molecule at a solution temperature that is lower than 75° C., such that the bio-molecule sits within the MOF framework as a bioactive, heterogeneous and discontinuous guest phase within a self-defined cavity.

Assignees

• Commw Scient Ind Res Org

Inventors

• Liang Kang
• Ricco Raffaele
• Doherty Cara Maxwell
• Falcaro Paolo

Classifications

• A61K47/6949

  inclusion complexes, e.g. clathrates, cavitates or fullerenes · CPC title

• A61K9/501 Primary

  Inorganic compounds · CPC title

• C12N11/04 Primary

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title

• C07H23/00

  Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12 (esters with inorganic acids C07H11/00) · CPC title

• C07K17/04 Primary

  entrapped within the carrier, e.g. gel, hollow fibre · CPC title