Compositions and methods for lipid production

Patent metadata

Field	Value
Publication number	US-10920233-B2
Application number	US-201715855237-A
Country	US
Kind code	B2
Filing date	Dec 27, 2017
Priority date	May 3, 2013
Publication date	Feb 16, 2021
Grant date	Feb 16, 2021

Abstract

Described herein, inter alia, are compositions, oleagnious organisms, and methods useful for producing lipids, lipid precursors, and/or oleochemicals.

First claim

What is claimed is:

1. A method of producing a lipid, lipid precursor, or oleochemi cal comprising:
1) culturing a genetically modified Yarrowia lipolytica yeast cell in a growth medium; and
2) isolating said lipid, lipid precursor, or oleochemical;
wherein the dry weight of said genetically modified yeast cell comprises greater than 60% wt/wt lipids, lipid precursors, and oleochemicals;
wherein said genetically modified yeast cell comprises (i) a recombinant acvl- CoA:diacvlglvcerol acvltransferase 1 (DGA1) gene and a genetically modified lipid synthesis regulator (MGA2) gene, wherein said genetically modified MGA2 gene increases lipid production compared to a non-genetically modified MGA2 control; or (ii) a recombinant acvl-CoA:diacvlglvcerol acvltransferase 2 (DGA2) gene and a genetically modified lipid synthesis regulator (MGA2) gene, wherein said genetically modified MGA2 gene increases lipid production compared to a non-genetically modified MGA2 control; and
wherein said lipid, lipid precursor, or oleochemical is not a sterol.

2. The method of claim 1 , wherein said growth medium comprises a majority carbon source selected from the group consisting of glucose, glycerol, xylose, fructose, mannose, ribose, sucrose, and lignocellulosic biomass.

3. The method of claim 1 , wherein said growth medium comprises lignocellulosic biomass as the majority carbon source.

4. The method of claim 1 , wherein said growth medium comprises cobalt, iron, magnesium, potassium, zinc, nickel, molybdenum, manganese, copper, or boron.

5. The method of claim 1 , wherein said growth medium comprises 5.77×10 −5 M to 1.73x10 −4 M cobalt, 0.001 M to 0.003 M magnesium, 4.52×10 −5 M to 1.35x10 −4 M potassium, 4.05×10 5 M to 1.22x10 −4 zinc, 3.55×10 −5 M to 1.06×10 −4 manganese, 9.07×10 −5 M to 2.91x10 −4 boron, 3.76×10 −5 M to 1.10×10 −4 molybdenum, 2.28×10 −5 M to 6.84×10 −5 nickel, 3.60×10 −5 M to 1.08x10 −4 iron, or 4.70×10 −5 M to 1.41x10 −4 copper.

6. The method of claim 1 , wherein said genetically modified MGA2 gene comprises at least one nucleotide modification.

7. The method of claim 1 , wherein said genetically modified MGA2 gene comprises SEQ ID NO: 51.

8. The method of claim 1 , wherein said genetically modified yeast cell further comprises a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MEFI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene or a recombinant AMP Deaminase (AMPD) gene.

9. The method of claim 1 , wherein said genetically modified yeast cell comprises a genetically modified multifunctional enzyme (MEFI) gene and a genetically modified PEX10 Transcription Factor (PEX10) gene.

10. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene and a genetically modified PEX10 Transcription Factor (PEX10) gene.

11. The method of claim 1 , wherein said genetically modified yeast cell comprises a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene and a recombinant AMP Deaminase (AMPD) gene.

12. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene and a recombinant AMP Deaminase (AMPD) gene.

Assignees

• Univ Texas

Inventors

• Blazeck John
• Hill Andrew
• Liu Leqian
• Alper Hal

Classifications

• C12R2001/645

  Fungi {; Processes using fungi} · CPC title

• C12N1/145

  Fungi isolates · CPC title

• C12P7/6409

  Fatty acids · CPC title

• C12N9/88

  Lyases (4.) · CPC title

• C12P7/6463

  obtained from glyceride producing microorganisms, e.g. single cell oil · CPC title