Compositions and methods for lipid production

Patent metadata

Field	Value
Publication number	US-9896691-B2
Application number	US-201414268796-A
Country	US
Kind code	B2
Filing date	May 2, 2014
Priority date	May 3, 2013
Publication date	Feb 20, 2018
Grant date	Feb 20, 2018

Abstract

Described herein, inter alia, are compositions, oleagnious organisms, and methods useful for producing lipids, lipid precursors, and/or oleochemicals.

First claim

What is claimed is:

1. A method of producing a lipid, lipid precursor, or oleochemical comprising:
a) culturing a genetically modified yeast cell in a growth medium; and
b) isolating said lipid, lipid precursor, or oleochemical,

wherein the dry weight of said genetically modified yeast cell comprises greater than 60% wt/wt lipids, lipid precursors, and oleochemicals; and
wherein said genetically modified yeast cell comprises (i) a recombinant acyl-CoA:diacylglycerol acyltransferase 1 (DGA1) gene and a UGA2 succinate semialdehyde dehydrogenase (UGA2) gene comprising a mutation, wherein said mutation results in a loss of function of succinate semialdehyde dehydrogenase of 20% or more compared to succinate semialdehyde dehydrogenase encoded by UGA2 without the mutation; or (ii) a recombinant acyl-CoA:diacylglycerol acyltransferase 2 (DGA2) gene and a UGA2 succinate semialdehyde dehydrogenase (UGA2) gene comprising a mutation, wherein said mutation results in a loss of function of succinate semialdehyde dehydrogenase of 20% or more compared to succinate semialdehyde dehydrogenase encoded by UGA2 without the mutation.

2. The method of claim 1 , wherein said growth medium comprises a majority carbon source selected from the group consisting of glucose, glycerol, xylose, fructose, mannose, ribose, sucrose, and lignocellulosic biomass.

3. The method of claim 1 , wherein said growth medium comprises lignocellulosic biomass as the majority carbon source.

4. The method of claim 1 , wherein said growth medium comprises cobalt, iron, magnesium, potassium, zinc, nickel, molybdenum, manganese, copper, or boron.

5. The method of claim 1 , wherein said growth medium comprises 5.77×10 −5 M to 1.73×10 −4 M cobalt, 0.001 M to 0.003 M magnesium, 4.52×10 −5 M to 1.35×10 −4 M potassium, 4.05×10 −5 M to 1.22×10 −4 zinc, 3.55×10 −5 M to 1.06×10 −4 manganese, 9.07×10 −5 M to 2.91×10 −4 boron, 3.76×10 −5 M to 1.10×10 −5 molybdenum, 2.28×10 −5 M to 6.84×10 −5 nickel, 3.60×10 −5 M to 1.08×10 −4 iron, or 4.70×10 −5 M to 1.41×10 −4 copper.

6. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Lipid synthesis regulator (MGA2) gene, a genetically modified Lipid synthesis regulator (MGA2) gene, a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene or a recombinant AMP Deaminase (AMPD) gene.

7. The method of claim 1 , wherein said genetically modified yeast cell comprises a genetically modified multifunctional enzyme (MFE1) gene and a genetically modified PEX10 Transcription Factor (PEX10) gene.

8. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene and a genetically modified PEX10 Transcription Factor (PEX10) gene.

9. The method of claim 1 , wherein said genetically modified yeast cell comprises a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene and a recombinant AMP Deaminase (AMPD) gene.

10. The method of claim 1 , wherein said genetically modified yeast cell comprises a recombinant Leucine Biosynthesis gene (LEU2), a genetically modified multifunctional enzyme (MFEI) gene, a genetically modified PEX10 Transcription Factor (PEX10) gene and a recombinant AMP Deaminase (AMPD)) gene.

Assignees

• Univ Texas

Inventors

• Blazeck John
• Hill Andrew
• Liu Leqian
• Alper Hal

Classifications

• C12P7/64

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title

• C12N9/93

  Ligases (6) · CPC title

• C12N15/815 Primary

  for yeasts other than Saccharomyces · CPC title

• C12N9/78

  acting on carbon to nitrogen bonds other than peptide bonds (3.5) · CPC title

• C12P7/6409

  Fatty acids · CPC title