Multiple emulsions created using jetting and other techniques

Patent metadata

Field	Value
Publication number	US-10874997-B2
Application number	US-201715656415-A
Country	US
Kind code	B2
Filing date	Jul 21, 2017
Priority date	Sep 2, 2009
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

The present invention generally relates to emulsions, and more particularly, to multiple emulsions. In one aspect, multiple emulsions are formed by urging a fluid into a channel, e.g., by causing the fluid to enter the channel as a “jet.” Multiple fluids may flow through a channel collinearly before multiple emulsion droplets are formed. The fluidic channels may also, in certain embodiments, include varying degrees of hydrophilicity or hydrophobicity. In some cases, the average cross-sectional dimension may change, e.g., at an intersection. Unexpectedly, systems such as those described herein may be used to encapsulate fluids in single or multiple emulsions that are difficult or impossible to encapsulate using other techniques, such as fluids with low surface tension, viscous fluids, or viscoelastic fluids. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like.

First claim

What is claimed is:

1. A method, comprising:
(a) providing a microfluidic device comprising a main microfluidic channel, at least one first side microfluidic channel, and at least one second side microfluidic channel;
(b) providing a first fluid in the main microfluidic channel;
(c) flowing the first fluid to a first intersection of the main microfluidic channel and the at least one first side microfluidic channel comprising a second fluid to cause the first fluid to become surrounded by the second fluid in the main microfluidic channel without causing the first fluid or the second fluid to form separate droplets;
(d) flowing the first fluid and the second fluid in the main microfluidic channel to a second intersection of the main microfluidic channel and the at least one second side microfluidic channel comprising a carrying fluid to cause the second fluid to become surrounded by the carrying fluid without causing the first fluid or the second fluid to form separate droplets; and
(e) generating a monodisperse double emulsion in the main microfluidic channel, wherein the monodisperse double emulsion is generated downstream of the second intersection,
wherein the monodisperse double emulsion comprises the carrying fluid surrounding an outer fluidic droplet of the second fluid, wherein the outer fluidic droplet contains an inner fluidic droplet of the first fluid;
wherein the first fluid is immiscible with the second fluid and the second fluid is immiscible with the carrying fluid;
wherein the main microfluidic channel has (i) a first average cross-sectional dimension upstream of the second intersection between the first intersection and the second intersection and a (ii) second average cross-sectional dimension downstream of the second intersection; and
wherein the second average cross-sectional dimension is between about 5% and about 20% greater than the first average cross-sectional dimension.

2. The method of claim 1 , wherein the first fluid is a first aqueous fluid, the second fluid is an oil, and the carrying fluid is a second aqueous fluid, and wherein the monodisperse double emulsion is a water-in-oil-in-water (w/o/w) emulsion.

3. The method of claim 2 , wherein the first fluid and the carrying fluid have the same composition.

4. The method of claim 2 , wherein the first fluid and the carrying fluid have a different composition.

5. The method of claim 1 , wherein the first fluid is a first oil, the second fluid is an aqueous fluid, and the carrying fluid is a second oil, and wherein the monodisperse double emulsion is an oil-in-water-in-oil (o/w/o) emulsion.

6. The method of claim 5 , wherein the first fluid and the carrying fluid have the same composition.

7. The method of claim 5 , wherein the first fluid and the carrying fluid have a different composition.

8. The method of claim 1 , wherein the main microfluidic channel has a first hydrophilicity upstream of the second intersection and a second hydrophilicity downstream of the second intersection; and wherein the second hydrophilicity is different than the first hydrophilicity.

9. The method of claim 1 , wherein the first fluid and the second fluid flow substantially collinearly in the main microfluidic channel upstream of the second intersection.

10. The method of claim 9 , wherein the first fluid, the second fluid, and the carrying fluid flow substantially collinearly in the main microfluidic channel downstream of the second intersection.

11. The method of claim 1 , wherein the first fluid has a surface tension of no more than about 40 mN/m (millinewtons per meter).

12. The method of claim 1 , wherein the second fluid has a surface tension at least about 2 times greater than a surface tension of the first fluid.

13. The method of claim 1 , wherein the first fluid comprises a viscosity of at least about 15 mPa s (millipascal-second).

14. The method of claim 1 , wherein the first fluid is a viscoelastic fluid.

15. The method of claim 1 , wherein the first fluid comprises a Young's modulus of at least about 0.01 GPa (gigapascal).

16. The method of claim 1 , wherein at least one of the first fluid, the second fluid, and the carrying fluid comprises at least one of a chemical, biochemical, or biological entity.

17. The method of claim 1 , wherein the inner fluidic droplet or the outer fluidic droplet comprises a particle.

18. The method of claim 1 , wherein the inner fluidic droplet or the outer fluidic droplet comprises a cell.

19. The method of claim 1 , wherein the inner fluidic droplet or the outer fluidic droplet comprises a nucleic acid molecule.

Assignees

• Harvard College

Inventors

• Weitz David A
• Thiele Julian W P
• Abate Adam R

Classifications

• B01F2215/0431

  Numerical size values, e.g. diameter of a hole or conduit, area, volume, length, width, or ratios thereof · CPC title

• F17D1/00

  Pipe-line systems (conveying articles or materials through a pipe- line by means of a fluid carrier B65G51/00, B65G53/00; liquids B67D; special devices for transferring liquids from bulk storage containers into vehicles or boats or vice versa, e.g. loading or unloading vehicles or portable containers B67D7/00; conveying material which has been excavated by a dredger or soil shifter through a pipe-line E02F7/10; sewer pipe-line systems E03F3/00; thermal insulation of pipe-lines F16L59/00; central heating systems F24D) · CPC title

• B01F35/80

  Forming a predetermined ratio of the substances to be mixed (controlling ratio of two or more flows of fluid or fluent material G05D11/02) · CPC title

• B01F25/90

  with moving or vibrating means, e.g. stirrers, for enhancing the mixing · CPC title

• B01F25/20 Primary

  Jet mixers, i.e. mixers using high-speed fluid streams (using jets to create vortex flow B01F25/10) · CPC title