Control of emulsions, including multiple emulsions

Patent metadata

Field	Value
Publication number	US-9573099-B2
Application number	US-201514961460-A
Country	US
Kind code	B2
Filing date	Dec 7, 2015
Priority date	May 23, 2011
Publication date	Feb 21, 2017
Grant date	Feb 21, 2017

Abstract

The present invention generally relates to emulsions, and more particularly, to double and other multiple emulsions. Certain aspects of the present invention are generally directed to the creation of double emulsions and other multiple emulsions at a common junction of microfluidic channels. In some cases, the microfluidic channels at the common junction may have substantially the same hydrophobicity. In one set of embodiments, a device may include a common junction of six or more channels, where a first fluid flows through one channel, a second fluid flows through two channels, and a third or carrying fluid flows through two more channels, such that a double emulsion of a first droplet of the first fluid, contained in a second droplet of the second fluid, contained by the carrying fluid, flows away from the common junction through a sixth channel. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like.

First claim

What is claimed is:

1. A method of creating an emulsion encapsulating a species, the method comprising:
providing a microfluidic device comprising a first junction of microfluidic channels comprising at least a first, second, and third microfluidic channels in fluidic communication, the first junction in fluid communication at an interface with a second junction of microfluidic channels comprising at least fourth, fifth, and sixth microfluidic channels in fluid communication, each of the first, second, and third microfluidic channels having a respective cross-sectional area at the first junction and each of the fourth, fifth, and sixth microfluidic channels having a respective cross-sectional area at a second junction, wherein the interface has a cross-sectional area smaller than the smallest cross-sectional areas of the fourth, fifth, and sixth microfluidic channels; and creating an emulsion encapsulating a species at the first and second junctions of microfluidic channels.

2. The method of claim 1 , wherein the emulsion is a double emulsion.

3. The method of claim 1 , wherein each of the microfluidic channels at the first and second junctions have substantially the same hydrophobicity.

4. The method of claim 1 , wherein one or more of the microfluidic channels at the first and second junctions have different hydrophobicity.

5. The method of claim 1 , wherein the species is: a particle, a chemical entity, a biochemical species, a biological entity, cells, a single cell, a pharmaceutical agent, drugs, a nucleic acid, proteins, a nanoparticle, quantum dots, fluorescent species or any combinations thereof.

6. The method of claim 5 , wherein the biochemical species is a nucleic acid.

7. The method of claim 6 , wherein the nucleic acid is: siRNA, RNAi, DNA or any combinations thereof.

8. The method of claim 5 , wherein the species is cells.

9. The method of claim 5 , wherein the species is a single cell.

10. The method of claim 5 , wherein the species is a particle and cells.

11. The method of claim 5 , wherein the species is a particle and a single cell.

Assignees

• Harvard College
• Basf Se

Inventors

• Weitz David A
• Rotem Assaf
• Abate Adam R
• Holtze Christian

Classifications

• Y10T137/87571

  Multiple inlet with single outlet · CPC title

• B01F3/0811 Primary

  Operations & Transport · mapped topic

• B01F3/0807

  Operations & Transport · mapped topic

• B01F13/0084

  Operations & Transport · mapped topic

• B01F13/0062

  Operations & Transport · mapped topic