Method for inducing differentiation of neural stem cells using patterned hydrogel

Patent metadata

Field	Value
Publication number	US-10870831-B2
Application number	US-201715736418-A
Country	US
Kind code	B2
Filing date	Mar 10, 2017
Priority date	Nov 11, 2016
Publication date	Dec 22, 2020
Grant date	Dec 22, 2020

Abstract

The present invention provides a method for inducing differentiation of neural stem cells. The present invention provides optimized differentiation conditions of neural stem cells into neurons using a patterned hydrogel.

First claim

What is claimed is:

1. A method for inducing differentiation of neural stem cells, the method comprising:
(a) culturing neural stem cells to induce neurospheres;
(b) seeding the neurospheres on a cell culture substrate on which a hydrogel comprising a biocompatible polymer and a conductive nanowire is patterned to have intervals of 150-250 μm; and
(c) applying voltage to the cell culture substrate to induce the differentiation into neurons,
wherein the conductive nanowires are patterned by mixing with the biocompatible polymer, and fixed by photocrosslinking of the biocompatible polymer, and
wherein the hydrogel in step (b) comprises a biocompatible polymer and a conductive nanowire at a mixing ratio of 8-12:1.

2. The method of claim 1 , wherein the neurospheres in step (a) have a size of 100-150 μm.

3. The method of claim 1 , wherein the biocompatible polymer in step (b) is polyethylene glycol (PEG).

4. The method of claim 1 , wherein the conductive nanowire in step (b) is a metal nanowire.

5. The method of claim 4 , wherein the metal nanowire is a silver nanowire, a copper nanowire, a gold nanowire, or an aluminum nanowire.

6. The method of claim 1 , wherein the voltage in step (c) is 8-12 V.

7. The method of claim 1 , wherein the cell culture substrate in step (c) has an average current value of 10-20 nA.

Assignees

• Univ Sogang Res Foundation

Inventors

• Chung Bong Geun
• Lee Jong Min
• Moon Joo Yoon
• Kim Tae Hyeon
• Ahrberg Daniel Christian

Classifications

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12N2529/00

  Culture process characterised by the use of electromagnetic stimulation · CPC title

• C12N2500/50

  Soluble polymers, e.g. polyethyleneglycol [PEG] · CPC title

• C12N2500/10

  Metals; Metal chelators · CPC title

• C12N5/0623

  Stem cells · CPC title