Performance gain in als inhibitor herbicide tolerant beta vulgaris plants by combination of best fitting als large and small subunits
US-2024389528-A1 · Nov 28, 2024 · US
US10865406B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10865406-B2 |
| Application number | US-201113821969-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 13, 2011 |
| Priority date | Oct 15, 2010 |
| Publication date | Dec 15, 2020 |
| Grant date | Dec 15, 2020 |
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The present invention relates to an ALS inhibitor herbicide tolerant Beta vulgaris plant and parts thereof comprising a mutation of an endogenous acetolactate synthase (ALS) gene, wherein the ALS gene encodes an ALS polypeptide containing an amino acid different from tryptophan at a position 569 of the ALS polypeptide.
Opening claim text (preview).
The invention claimed is: 1. A method for manufacturing an ALS inhibitor herbicide tolerant Beta vulgaris plant or parts thereof, comprising: (a) exposing calli from B. vulgaris, to about 10 −7 M-10 −9 M of foramsulfuron; (b) selecting callus tissue from said calli, wherein said callus tissue can grow in the presence of up to 3×10 −6 M foramsulfuron; (c) regenerating shoots from said callus tissue in presence of foramsulfuron; (d) regenerating plantlets from said shoots; (e) selecting the regenerated plantlets with foramsulfuron, iodosulfuron-methyl-sodium and/or a mixture of both, wherein the plantlets comprise a mutation at a position corresponding to nucleotides 1705-1707 of the endogenous acetolactate synthase (ALS) gene shown in reference sequence SEQ ID NO: 1, wherein the mutated ALS gene encodes an ALS polypeptide containing leucine at position 569 of the ALS polypeptide shown in reference amino acid sequence SEQ ID NO: 2, and wherein the plantlets are homozygous for the mutation of the endogenous ALS gene.
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