Gene for resistance to plant disease

Patent metadata

Field	Value
Publication number	US-12098377-B2
Application number	US-201917429674-A
Country	US
Kind code	B2
Filing date	Feb 18, 2019
Priority date	Feb 18, 2019
Publication date	Sep 24, 2024
Grant date	Sep 24, 2024

Abstract

A more efficient breeding against plant disease, or the development of new resistant lines, is enabled via the provision of the resistance-mediating gene according to the invention; in particular, a resistance effect in the target plant is evoked by the property of the identified gene. The resistance-mediating gene, and embodiments of the present invention that are described in the preceding, offer additional applications, e.g., the use of the resistant gene allele in trans-genetic approaches, with the goal of developing new resistant cultivars.

First claim

The invention claimed is:

1. A nucleic acid molecule which encodes a polypeptide that is able to confer resistance to Cercospora beticola in a Beta vulgaris subsp. vulgaris plant in which the polypeptide is expressed, wherein the nucleic acid molecule comprises
the DNA sequence according to SEQ ID NO: 2.

2. A vector or expression cassette comprising the nucleic acid molecule according to claim 1 .

3. A cell which comprises the nucleic acid molecule according to claim 1 .

4. A Cercospora beticola -resistant Beta vulgaris subsp. vulgaris plant or a portion thereof, wherein the plant or its portion contains the nucleic acid molecule according to claim 1 transgenically.

5. A seed or descendant of the Cercospora beticola -resistant Beta vulgaris subsp. vulgaris plant according to claim 4 , wherein the seed or the descendant comprises the nucleic acid molecule transgenically.

6. The seed according to claim 5 , which has been technically treated, wherein the technical treatment is selected from the group consisting of:
(a) polishing;
(b) dressing;
(c) incrustation; and
(d) coloring.

7. A method for increasing the resistance to Cercospora beticola in a plant of the species Beta vulgaris subsp. vulgaris comprising integrating the nucleic acid molecule according to claim 1 by means of homology-directed repair or homologous recombination into the genome of at least one cell of the Beta vulgaris subsp. vulgaris , and optional regeneration of a plant from the plant cell.

8. A method for producing a Cercospora beticola -resistant Beta vulgaris subsp. vulgaris plant according to claim 5 , including the following steps:
(a) introducing a site-directed nuclease and a repair matrix into a cell of the Beta vulgaris subsp. vulgaris plant, wherein the site-directed nuclease is able to generate at least one double-strand break of the DNA in the genome of the cell and the repair matrix comprises the nucleic acid molecule;
(b) cultivating the cell from (a) under conditions that allow a homology-directed repair or a homologous recombination, wherein the nucleic acid molecule is integrated from the repair matrix into the genome of the plant; and
(c) regenerating a plant from the cell modified in (b).

9. The method according to claim 8 , wherein the at least one double strand break occurs in a sensitive allelic variant of the nucleic acid molecule or in that the at least one double strand break occurs at a position which is at most 10,000 base pairs upstream or downstream away from the sensitive allelic variant, wherein the allelic variant encodes a polypeptide which does not confer resistance to Cercospora beticola.

10. The method according to claim 9 , wherein the sensitive allelic variant comprises a nucleotide sequence, which is selected from the group consisting of:
(a) a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence according to SEQ ID NO: 6;
(b) a nucleotide sequence which comprises the sequence according to SEQ ID NO: 5;
(c) a nucleotide sequence which comprises the sequence according to SEQ ID NO: 4; and
(d) a nucleotide sequence which encodes a polypeptide which differs from a polypeptide which is encoded by the nucleotide sequence according to (a), (b) or (c) by conservative substitution of one or more amino acids of the amino acid sequence.

11. A method for identifying, and optionally providing, a Beta vulgaris subsp. vulgaris plant that is resistant to Cercospora beticola , wherein the method comprises detecting the presence and/or expression of the nucleic acid molecule according to claim 1 in the plant or a portion of the plant and, optionally, selecting the Cercospora beticola -resistant plant.

12. A method for cultivating plants of the species Beta vulgaris subsp. vulgaris , the method comprising:
(i) providing plants according to claim 5 , and
(ii) cultivating the plants from (i) or descendants thereof,
wherein the method counteracts an infestation of the cultivated plants with Cercospora beticola.

Assignees

• Kws Saat Se & Co Kgaa

Inventors

• Torjek Otto
• Borchardt Dietrich
• Rekoske Margaret
• Mechelke Wolfgang
• Lein Jens Christoph
• Schulz Britta

Classifications

• C12N15/8213

  Targeted insertion of genes into the plant genome by homologous recombination · CPC title

• C07K14/415

  from plants · CPC title

• A01H6/024

  Beta vulgaris [beet] · CPC title

• A01H5/06

  Roots · CPC title

• C12N15/8282 Primary

  for fungal resistance · CPC title