Contrast agents, methods for preparing contrast agents, and methods of imaging

Patent metadata

Field	Value
Publication number	US-10849993-B2
Application number	US-201815910893-A
Country	US
Kind code	B2
Filing date	Mar 2, 2018
Priority date	Apr 2, 2008
Publication date	Dec 1, 2020
Grant date	Dec 1, 2020

Abstract

Embodiments of the present disclosure provide for contrast agents, methods of making contrast agents, and methods of using contrast agents, and the like.

First claim

We claim the following:

1. A contrast agent comprising a modified parvalbumin scaffold polypeptide, wherein one or more amino acids of the scaffold polypeptide form at least one paramagnetic metal ion chelating site, and further comprising a paramagnetic metal ion bound to at least two of the amino acids forming the at least one paramagnetic metal ion chelating site,
wherein the modified parvalbumin scaffold polypeptide has at least one amino acid substitution that modifies at least one of the metal selectivity of the binding site, increases binding affinity for the metal, or increases serum stability of the contrast agent, and wherein the at least one amino acid substitution is selected from the group consisting of S56D, E60D, G99D, and F103W, wherein the amino acid positions are numbered according to SEQ ID NO: 38, and wherein the modified parvalbumin scaffold polypeptide having the paramagnetic metal ion bound thereto is detectable by magnetic resonance imaging (MRI).

2. The contrast agent of claim 1 , wherein the modified parvalbumin scaffold polypeptide has an amino acid sequence having at least 90% similarity to any of SEQ ID Nos: 28-38.

3. The contrast agent of claim 1 , wherein the modified parvalbumin scaffold polypeptide has an amino acid sequence selected from the group consisting of SEQ ID Nos: 28-38.

4. The contrast agent of claim 1 , including at least one post-translational modification.

5. The contrast agent of claim 1 , further comprising at least one polyethylene glycol (PEG) group or glucan group attached to the modified parvalbumin scaffold polypeptide.

6. The contrast agent of claim 1 , wherein at least two metal ion chelating sites are comprised of amino acids of the modified parvalbumin scaffold polypeptide, and wherein each of the metal ion chelating sites has a paramagnetic ion independently bound thereto.

7. The contrast agent of claim 1 , further comprising a targeting agent attached to the polypeptide, wherein the targeting agent is a biomarker probe, a precancerous targeting agent, a cancer targeting agent, a tumor targeting agent, a fibrosis targeting agent, or a detectable probe or agent that targets a biomarker, a precancerous cell, a cancer cell, or a tumor.

8. The contrast agent of claim 5 , wherein the PEG has a molecular weight of about 1 to 100 kDa.

9. The contrast agent of claim 5 , wherein the PEG is attached to the modified parvalbumin scaffold polypeptide via a lysine, a glutamic acid, a aspartic acid, a cysteine, the polypeptide carboxy-terminus, the polypeptide amino-terminus, or a combination thereof.

10. The contrast agent of claim 5 , wherein at least one metal ion chelating site is at least substantially embedded within the modified parvalbumin scaffold polypeptide.

11. The contrast agent of claim 5 , wherein the paramagnetic metal ion is selected from the group consisting of: Gd(III), Mn(II), Fe(II), Fe(III), Co(II), Co(III), Ni(III), Mo(V), and V(IV).

12. The contrast agent of claim 11 , wherein the paramagnetic metal ion is Gd(III).

13. The contrast agent of claim 7 , wherein the targeting agent targets to Her-2, EGFR, GRPR, integrin, or collagen.

14. The contrast agent of claim 7 , wherein the targeting agent is a detectable Near-IR functional group covalently bound to the modified parvalbumin scaffold polypeptide.

15. A method of imaging a sample comprising:
contacting a sample with a composition comprising a contrast agent comprising a modified parvalbumin scaffold polypeptide, wherein one or more amino acids of the scaffold polypeptide form at least one paramagnetic metal ion chelating site, and further comprising a paramagnetic metal ion bound to at least two of the amino acids forming the at least one paramagnetic metal ion chelating site,
wherein the modified parvalbumin scaffold polypeptide has at least one amino acid substitution that modifies at least one of the metal selectivity of the binding site, increases binding affinity for the metal, or increases serum stability of the contrast agent, and wherein the at least one amino acid substitution is selected from the group consisting of S56D, E60D, G99D, and F103W, wherein the amino acid positions are numbered according to SEQ ID NO: 38, and wherein the modified parvalbumin scaffold polypeptide having the paramagnetic metal ion bound thereto is detectable by magnetic resonance imaging (MRI);
introducing the sample contacted with the composition to an imaging system;
detecting a signal generated by or from the contrast agent of the introduced sample; and
generating an image of the sample from the signal.

16. The method of claim 15 , wherein the modified parvalbumin scaffold polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID Nos: 28-38.

Assignees

• Georgia State Univ Research Foundation
• Univ Georgia State Res Found

Inventors

• Yang Jenny Jie
• Liu Zhiren
• Jiang Jie
• Xue Shenghui
• Qiao Jinjuan
• Salarian Mani

Classifications

• A61K49/14 Primary

  Peptides, e.g. proteins · CPC title

• A61K49/143

  the protein being an albumin, e.g. HSA, BSA, ovalbumin · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61K49/126

  Linear polymers, e.g. dextran, inulin, PEG · CPC title