Microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate and method for preparing 5′-xanthosine monophosphate using the same

Patent metadata

Field	Value
Publication number	US-10844348-B2
Application number	US-201816465774-A
Country	US
Kind code	B2
Filing date	Jun 21, 2018
Priority date	Jun 7, 2018
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

The present disclosure relates to a microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate and a method for producing 5′-xanthosine monophosphate using the same.

First claim

The invention claimed is:

1. A microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate, wherein activity of a protein comprising the amino acid sequence of SEQ ID NO: 2 is enhanced.

2. The microorganism of the genus Corynebacterium of claim 1 , wherein the protein is encoded by a gene comprising the nucleotide sequence of SEQ ID NO: 1.

3. The microorganism of the genus Corynebacterium of claim 1 , wherein the enhancement of activity is achieved by an increase in the number of copies of polynucleotides encoding the protein, enhancement of promoter activity, substitution of a start codon, or a combination thereof.

4. The microorganism of the genus Corynebacterium of claim 1 , wherein the microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate is Corynebacterium stationis.

5. A method for producing 5′-xanthosine monophosphate, comprising:
culturing the microorganism of the genus Corynebacterium of claim 1 in a culture medium; and
recovering the 5′-xanthosine monophosphate from the microorganism or the culture medium.

6. The method for producing 5′-xanthosine monophosphate of claim 5 , wherein the microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate is Corynebacterium stationis.

7. A method for producing 5′-xanthosine monophosphate, comprising:
culturing the microorganism of the genus Corynebacterium of claim 2 in a culture medium; and
recovering the 5′-xanthosine monophosphate from the microorganism or the culture medium.

8. A method for producing 5′-xanthosine monophosphate, comprising:
culturing the microorganism of the genus Corynebacterium of claim 3 in a culture medium; and
recovering the 5′-xanthosine monophosphate from the microorganism or the culture medium.

9. A method for producing 5′-xanthosine monophosphate, comprising:
culturing the microorganism of the genus Corynebacterium of claim 4 in a culture medium; and
recovering the 5′-xanthosine monophosphate from the microorganism or the culture medium.

10. The method for producing 5′-xanthosine monophosphate of claim 7 , wherein the microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate is Corynebacterium stationis.

11. The method for producing 5′-xanthosine monophosphate of claim 8 , wherein the microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate is Corynebacterium stationis.

12. The method for producing 5′-xanthosine monophosphate of claim 9 , wherein the microorganism of the genus Corynebacterium producing 5′-xanthosine monophosphate is Corynebacterium stationis.

Assignees

• Cj Cheiljedang Corp

Inventors

• Baek Min Ji
• Lee Ji Hye
• Lim Boram
• Yoon Byoung Hoon
• Lee Jeong Eun
• Lim Su-Bin
• Jeong Jaeho

Classifications

• C12P19/32 Primary

  having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide · CPC title

• C07K14/34

  from Corynebacterium (G) · CPC title

• C12P19/40

  having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides · CPC title

• C12N15/67

  General methods for enhancing the expression · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title