Lipid composition and method for producing same

Patent metadata

Field	Value
Publication number	US-10844317-B2
Application number	US-201916273499-A
Country	US
Kind code	B2
Filing date	Feb 12, 2019
Priority date	Mar 18, 2015
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

The method of producing a lipid composition comprises an extraction treatment step to extract a lipid comprised in a hydrated raw material by using an extraction solvent comprising a polar solvent and a nonpolar solvent, and a separation treatment step to separate an extract solution obtained in the extraction treatment step into a polar solvent phase comprising a first lipid fraction and a nonpolar solvent phase comprising a second lipid fraction.

First claim

What is claimed is:

1. A method of producing a lipid composition, the method comprising:
an extraction treatment step to extract a lipid comprised in a hydrated raw material by using an extraction solvent comprising a polar solvent and a nonpolar solvent;
a separation treatment step to separate an extract solution obtained in the extraction treatment step into a polar solvent phase comprising a first lipid fraction and a nonpolar solvent phase comprising a second lipid fraction; and
a purification treatment step to remove an impurity from the nonpolar solvent phase by contacting the nonpolar solvent phase obtained in the separation treatment step with an adsorbent;
wherein the hydrated raw material comprises at least one byproduct of aquatic product processing selected from midgut gland, gonad, mantle, and gill of a Hotate-gai and eyeball, buccal bulb, and skin of a squid.

2. The method of producing a lipid composition according to claim 1 , further comprising a solvent recovery step to recover the polar solvent and the nonpolar solvent from the polar solvent phase and the nonpolar solvent phase obtained in the separation treatment step, respectively.

3. The method of producing a lipid composition according to claim 1 , wherein the polar solvent comprises a lower alcohol, acetone, acetonitrile, THF, DMF, or a combination thereof.

4. The method of producing a lipid composition according to claim 1 , wherein the nonpolar solvent comprises an alkane with a carbon number of 5 to 8, diethyl ether, t-butyl methyl ether, ethyl acetate, chloroform, methylene chloride, benzene, toluene, or a combination thereof.

5. The method of producing a lipid composition according to claim 1 , wherein no lower than 3% by mass of the total lipid comprised in the hydrated raw material is phospholipid.

6. The method of producing a lipid composition according to claim 1 wherein, for the extraction treatment step, the extraction solvent is prepared beforehand as a mixed organic solvent using the polar solvent and the nonpolar solvent and then added to the hydrated raw material, or, the polar solvent and the nonpolar solvent are added individually and sequentially to the hydrated raw material to prepare the mixed organic solvent by virtue of the additions.

7. The method of producing a lipid composition according to claim 1 , wherein the hydrated raw material comprises water at a water content of 60 to 85% by weight.

8. The method of producing a lipid composition according to claim 1 wherein, in the extraction treatment step, the polar solvent and the nonpolar solvent are used at 1 to 5 parts by weight and 1 to 10 parts by weight, respectively, relative to 1 part by wet weight of the hydrated raw material.

9. The method of producing a lipid composition according to claim 1 , further comprising a heat treatment step to heat the hydrated raw material at a temperature of 65° C. or higher.

10. A method of producing a lipid composition, the method comprising:
a wet crushing step to crush a hydrated raw material;
an extraction treatment step to extract a lipid comprised in the hydrated raw material by using an extraction solvent comprising a polar solvent and a nonpolar solvent; and
a separation treatment step to separate an extract solution obtained in the extraction treatment step into a polar solvent phase comprising a first lipid fraction and a nonpolar solvent phase comprising a second lipid fraction;
wherein the hydrated raw material comprises at least one byproduct of aquatic product processing selected from midgut gland, gonad, mantle, and gill of a Hotate-gai and eyeball, buccal bulb, and skin of a squid.

11. The method of producing a lipid composition according to claim 10 , further comprising a solvent recovery step to recover the polar solvent and the nonpolar solvent from the polar solvent phase and the nonpolar solvent phase obtained in the separation treatment step, respectively.

12. The method of producing a lipid composition according to claim 10 , wherein the polar solvent comprises a lower alcohol, acetone, acetonitrile, THF, DMF, or a combination thereof.

13. The method of producing a lipid composition according to claim 10 , wherein the nonpolar solvent comprises an alkane with a carbon number of 5 to 8, diethyl ether, t-butyl methyl ether, ethyl acetate, chloroform, methylene chloride, benzene, toluene, or a combination thereof.

14. The method of producing a lipid composition according to claim 10 , wherein no lower than 3% by mass of the total lipid comprised in the hydrated raw material is phospholipid.

15. The method of producing a lipid composition according to claim 10 wherein, for the extraction treatment step, the extraction solvent is prepared beforehand as a mixed organic solvent using the polar solvent and the nonpolar solvent and then added to the hydrated raw material, or, the polar solvent and the nonpolar solvent are added individually and sequentially to the hydrated raw material to prepare the mixed organic solvent by virtue of the additions.

16. The method of producing a lipid composition according to claim 10 , wherein the hydrated raw material comprises water at a water content of 60 to 85% by weight.

17. The method of producing a lipid composition according to claim 10 wherein, in the extraction treatment step, the polar solvent and the nonpolar solvent are used at 1 to 5 parts by weight and 1 to 10 parts by weight, respectively, relative to 1 part by wet weight of the hydrated raw material.

18. The method of producing a lipid composition according to claim 10 , further comprising a heat treatment step to heat the hydrated raw material at a temperature of 65° C. or higher.

19. A method of producing a lipid composition, the method comprising:
a wet crushing step to crush a hydrated raw material into a paste;
and following the wet crushing step, an extraction treatment step to extract a lipid comprised in the hydrated raw material by using an extraction solvent comprising a polar solvent and a nonpolar solvent in combination, wherein the polar solvent is a lower alcohol and the nonpolar solvent is an alkane with a carbon number of 5 to 8; and
a separation treatment step to separate by centrifugation an extract solution obtained in the extraction treatment step into a polar solvent phase comprising a first lipid fraction, a nonpolar solvent phase comprising a second lipid fraction, and a solid fraction phase;
wherein the hydrated raw material comprises water at a water content of 60 to 85% by weight, and
wherein the hydrated raw material comprises at least one byproduct of aquatic product processing selected from midgut gland, gonad, mantle, and gill of a Hotate-gai.

20. The method of producing a lipid composition according to claim 19 , wherein the polar solvent is ethanol and the nonpolar solvent is n-hexane.

Assignees

• Ihi Corp

Inventors

• Okuyama Junichi
• Yajima tomomi
• Takahashi Koretaro
• Yoshioka Takeya

Classifications

• C12P7/6481

  Phosphoglycerides (phosphoglycerides having carboxylic acids with less than seven carbon atoms C12P7/62) · CPC title

• C12P7/6472

  containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone · CPC title

• C12P7/6463

  obtained from glyceride producing microorganisms, e.g. single cell oil · CPC title

• C11B13/00

  Recovery of fats, fatty oils or fatty acids from waste materials · CPC title

• C11B3/12

  by distillation · CPC title