Method for producing biomass which has a high exopolysaccharide content

Patent metadata

Field	Value
Publication number	US-10842174-B2
Application number	US-201515516058-A
Country	US
Kind code	B2
Filing date	Oct 2, 2015
Priority date	Oct 2, 2014
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

According to the invention, it was found that culturing of cells of the taxon Labyrinthulomycetes in a high content of sulphate makes it possible to obtain a biomass having a high EPS content, which biomass can in addition be advantageously further processed into a feedstuff.

First claim

The invention claimed is:

1. A biomass dried comprising: exopolysaccharides (EPSs) of microorganisms of the taxon Labyrinthulomycetes, wherein the biomass has and a sulphate content, based on the dry mass, of 25 to 60 g/kg.

2. The biomass of claim 1 , wherein said biomass has a sulphate content, based on the dry mass, of 25 to 40 g/kg.

3. The biomass of claim 1 , wherein the microorganisms of the taxon Labyrinthulomycetes are of the family Thraustochytriaceae.

4. The biomass of claim 3 , wherein the microorganisms of the taxon Labyrinthulomycetes are of the genus Althomia, Aplanochytrium, Elnia, Japonochytrium, Schizochytrium, Thraustochytrium, Aurantiochytrium, Oblongichytrium or Ulkenia.

5. The biomass of claim 4 , wherein the microorganisms are of the genus Aurantiochytrium.

6. The biomass of claim 5 , wherein the microorganisms are of species Aurantiochytrium limacinum.

7. A feedstuff, comprising the biomass of claim 1 .

8. The feedstuff of claim 7 , wherein said feedstuff comprises:
a) a total protein content of 30 to 60% by weight;
b) a total fat content of 15 to 35% by weight;
c) a total starch content of at most 25% by weight; and
d) a biomass content of 2 to 22% by weight.

9. The feedstuff of claim 8 , wherein said feedstuff comprises:
e) a polyunsaturated fatty acid (PUFA) content of 2 to 12% by weight.

10. The feedstuff of claim 8 , wherein said feedstuff comprises:
f) an omega-3 fatty acid content of 1 to 6% by weight.

11. The feedstuff of claim 8 , wherein said feedstuff comprises:
g) a DHA content of 0.5 to 3% by weight.

12. The feedstuff of claim 9 , wherein said feedstuff comprises:
f) an omega-3 fatty acid content of 1 to 6% by weight; and
g) a DHA content of 0.5 to 3% by weight.

13. The feedstuff of claim 7 , wherein said feedstuff comprises:
a) a total protein content of 40 to 50% by weight;
b) a total fat content of 20 to 30% by weight;
c) a total starch content of 5 to 15% by weight;
d) a biomass content of 8 to 18% by weight.

14. The feedstuff of claim 13 , further comprising one or more of the following:
e) a polyunsaturated fatty acid (PUFA) content of 5 to 8% by weight;
f) an omega-3 fatty acid content of 2.5 to 4% by weight;
g) a DHA content of 1.2 to 2.0% by weight.

15. The biomass of claim 1 , comprising a sulphate content, based on the dry mass, of 25 to 30 g/kg.

16. The biomass of claim 15 , wherein the microorganisms of the taxon Labyrinthulomycetes are of the family Thraustochytriaceae.

17. The biomass of claim 15 , wherein the microorganisms are cells of the species Aurantiochytrium limacinum.

18. The feedstuff of claim 8 , wherein said biomass comprises a sulphate content, based on the dry mass, of 25 to 30 g/kg.

19. The feedstuff of claim 18 , wherein said feedstuff comprises: a polyunsaturated fatty acid (PUFA) content of 2 to 12% by weight.

20. The feedstuff of claim 19 , wherein said feedstuff comprises an omega-3 fatty acid content of 1 to 6% by weight.

Assignees

• Evonik Degussa Gmbh
• Evonik Operations Gmbh

Inventors

• Durhuus Thomas
• Eils Stefan
• Silva Amelia Claudia
• Priefert Horst
• Schneider Jens
• Rabe Christian

Classifications

• A61K35/66

  Microorganisms or materials therefrom (fungi, yeasts or candida A61K36/06) · CPC title

• A61K31/715

  Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters · CPC title

• C12P7/64

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title

• A61K31/202 Primary

  having three or more double bonds, e.g. linolenic (eicosanoids, e.g. leukotrienes A61K31/557) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title