Apparatus and method for separating whey proteins from whey using the same

Patent metadata

Field	Value
Publication number	US-10842165-B2
Application number	US-201716099921-A
Country	US
Kind code	B2
Filing date	May 11, 2017
Priority date	May 11, 2016
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

The present invention provides a method for the production of whey proteins in a single step process using combination of chromatography and membrane filtration technique, comprising treating cotton cloth with a mixture of chlorosulphonic acid and chloroform and then subsequently treating it with chloroform, dilute NaOH, glycine and water to recover modified cotton cloth as the product, thereafter fixing product in a membrane filtration device equipped with modified flow pattern and then equilibrating it with equilibration buffer, followed by loading of whey for adsorption of protein on the product and washing of the product with equilibration buffer, thereafter elution of adsorbed proteins with elution buffer, and then regeneration of the product by treating it with dilute HCl and water to reuse the product.

First claim

We claim:

1. A system for separating whey proteins from whey, said system comprising:
a filtration unit ( 11 ) comprising a bottom plate ( 12 ) at a first extremity, a top plate ( 13 ) at a second extremity opposite to the first extremity, a permeate spacer ( 14 ) placed proximate to and in spaced apart relation with respect to the bottom plate ( 12 ), at least one porous filter ( 15 ) placed proximate to and in spaced apart relation with respect to the permeate spacer ( 13 ) and a gasket ( 16 ) provided between the porous filter ( 15 ) and the top plate ( 13 ); the filtration unit defining an input port ( 17 ), a permeate port ( 18 ) and a retentate port ( 19 ); the porous filter comprising cotton cloth having an ion exchange element deposited thereupon;
a feed storage unit ( 20 ) connected to the input port ( 17 ) of the filtration unit via a first pump ( 21 );
an eluent storage unit ( 22 ) connected to the input port ( 17 ) of the filtration unit via a second pump ( 23 );
a first set of tubing ( 24 ) comprising an input end ( 25 ), a first output end ( 26 ), a second end ( 27 ), a first valve ( 28 ) for controlling an amount of fluid flowing out of the first output end ( 26 ) and a second valve ( 29 ) for controlling an amount of fluid flowing out of the second output end ( 27 ), the input end being ( 25 ) connected to the permeate port ( 18 ), the first output end ( 26 ) being connected to the feed storage unit ( 20 ) and the second output end ( 27 ) being connected to the eluent storage unit ( 22 ); and
a second set of tubing ( 30 ) comprising a input end ( 31 ), a first output end ( 32 ), a second end ( 33 ), a first valve ( 34 ) for controlling an amount of fluid flowing out of the first output end ( 32 ) and a second valve ( 35 ) for controlling an amount of fluid flowing out of the second output end ( 33 ), the input end ( 31 ) being connected to the retentate port ( 19 ), the first output end ( 32 ) being connected to the feed storage unit ( 20 ) and the second output end ( 33 ) being connected to the eluent storage unit ( 22 ).

2. The system as claimed in claim 1 , wherein the second set of tubing ( 30 ) further comprises:
a first pressure sensor ( 36 ) located between the first valve ( 34 ) and the first output end ( 32 );
a first flow rate sensor ( 37 ) located between the first pressure sensor ( 36 ) and the first output end ( 32 );
a third valve ( 38 ) located between the first flow rate sensor ( 36 ) and the first pressure sensor ( 36 );
a second pressure sensor ( 39 ) located between the second valve ( 35 ) and the second output end ( 33 );
a second flow rate sensor ( 40 ) located between the second pressure sensor ( 39 ) and the second output end ( 33 ); and
a fourth valve ( 41 ) located between the second flow rate sensor ( 40 ) and the second pressure sensor ( 39 ).

3. The system as claimed in claim 2 , further comprising a third tubing set ( 42 ) connecting the feed storage unit ( 20 ) to the input port ( 17 ) of the filtration unit ( 11 ) via the first pump ( 21 ) and connecting the eluent storage unit ( 22 ) connected to the input port ( 17 ) of the filtration unit ( 11 ) via the second pump ( 23 ).

4. The system as claimed in claim 3 , wherein the third tubing set ( 42 ) comprises:
an output end ( 43 ) connected to the filtration unit ( 11 );
an intermediate portion ( 44 );
a first input end ( 45 ) connected to the feed storage unit ( 20 );
a second input end ( 46 ) connected to the eluent storage unit ( 22 );
a first input section ( 47 ) located between the intermediate portion ( 44 ) and the first input end ( 45 ) and accommodating the first pump ( 21 );
a second input section ( 48 ) located between the intermediate portion ( 44 ) and the second input end ( 46 ) and accommodating the second pump ( 23 );
a first control valve ( 49 ) located between the feed storage unit ( 20 ) and the first pump ( 21 );
a second control valve ( 50 ) located between the first pump ( 21 ) and the intermediate portion ( 44 );
a third control valve ( 51 ) located between the eluent storage unit ( 22 ) and the second pump ( 23 ); and
a fourth control valve ( 52 ) located between the second pump ( 23 ) and the intermediate portion ( 44 ).

5. The system as claimed in claim 4 , further comprising a control unit ( 53 ) adapted to control operation of the first pump ( 21 ) and the second pump ( 23 ).

6. The system as claimed in claim 5 , wherein the control unit ( 53 ) is further adapted to control operation of the first valve ( 28 ) forming part of the first tubing set ( 24 ); the second valve ( 29 ) forming part of the first tubing set ( 24 ); the first valve ( 34 ) forming part of the second tubing set ( 30 ); the second valve ( 35 ) forming part of the second tubing set ( 30 ); the third valve ( 38 ) forming part of the second tubing set ( 30 ); the fourth valve ( 41 ) forming part of the second tubing set ( 30 ); the first control valve ( 49 ) forming part of the third tubing set ( 42 ); the second control valve ( 50 ) forming part of the third tubing set ( 42 ); the third control valve ( 51 ) forming part of the third tubing set ( 42 ); and the fourth control valve ( 52 ) forming part of the third tubing set ( 42 ) such that:
during operation of the first pump ( 21 ), the third control valve ( 51 ) forming part of the third tubing set ( 42 ); and the fourth control valve ( 52 ) forming part of the third tubing set ( 42 ); the second valve ( 29 ) forming part of the first tubing set ( 24 ); the second valve ( 35 ) forming part of the second tubing set ( 30 ); and the fourth valve ( 41 ) forming part of the second tubing set ( 30 ) are maintained in a closed state; and
during operation of the first pump ( 21 ), the first control valve ( 49 ) forming part of the third tubing set ( 42 ); the second control valve ( 50 ) forming part of the third tubing set ( 42 ); the first valve ( 28 ) forming part of the first tubing set ( 24 ); the first valve ( 34 ) forming part of the second tubing set ( 30 ); and the third valve ( 38 ) forming part of the second tubing set ( 30 ) are controlled to be in any one of an open state or a closed state;
during operation of the second pump ( 23 ), the first control valve ( 49 ) forming part of the third tubing set ( 42 ); the second control valve ( 50 ) forming part of the third tubing set ( 42 ); the first valve ( 28 ) forming part of the first tubing set ( 24 ); the first valve ( 34 ) forming part of the second tubing set ( 30 ); and the third valve ( 38 ) forming part of the second tubing set ( 30 ) are maintained in a closed state; and
during operation of the second pump ( 23 ), the third control valve ( 51 ) forming part of the third tubing set ( 42 ); and the fourth control valve ( 52 ) forming part of the third tubing set ( 42 ); the second valve ( 29 ) forming part of the first tubing set ( 24 ); the second valve ( 35 ) forming part of the second tubing set ( 30 ); and the fourth valve ( 41 ) forming part of the second tubing set ( 30 ) are controlled to be in any one of an open state or a closed state.

7. The system as claimed in claim 1 , wherein the first pump has a first speed and the second pump has a second speed, with the second speed being lesser than the first speed.

8. A process for separating whey proteins, comprising the steps of:
(a) providing a system comprising:
a filtration unit ( 11 ) comprising a bottom plate ( 12 ) at a first extremity, a top plate ( 13 ) at a second extremity opposite to the first extremity, a permeate spacer ( 14 ) placed proximate to and in spaced apart relation with respect to the bottom plate ( 12 ), at least one porous filter ( 15 ) placed proximate to and in spaced apart relation with respect to the permeate spacer ( 13 ) and a gasket ( 16 ) provided betwe

Assignees

• Council Scient Ind Res

Inventors

• Adikane Harshavardhan Vishavanath
• Jagtap Mahendra Devram

Classifications

• B01D37/025

  additives incorporated in the filter · CPC title

• A23J1/205

  from whey, e.g. lactalbumine · CPC title

• A23C9/1465

  Chromatographic separation of protein or lactose fraction; Adsorption of protein or lactose fraction followed by elution · CPC title

• B01J47/014

  in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds · CPC title

• B01J39/22

  Cellulose or wood; Derivatives thereof · CPC title