Method for producing coenzyme Q10

Patent metadata

Field	Value
Publication number	US-10837043-B2
Application number	US-201816233392-A
Country	US
Kind code	B2
Filing date	Dec 27, 2018
Priority date	Jul 1, 2016
Publication date	Nov 17, 2020
Grant date	Nov 17, 2020

Abstract

A method of producing coenzyme Q10 includes contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained. The adsorbent (A) includes aluminum silicate at a content of 50% or more.

First claim

What is claimed is:

1. A method of producing coenzyme Q10, the method comprising:
contacting an extract comprising coenzyme Q10 from a coenzyme Q10-producing microorganism with an adsorbent (A), wherein the adsorbent (A) adsorbs components of the extract other than coenzyme Q10, and a solution of the coenzyme Q10 having a higher purity compared to the extract is obtained,
wherein the adsorbent (A) comprises 50% or more of aluminum silicate.

2. The method of claim 1 , further comprising:
before or after contacting the extract with the adsorbent (A), contacting the extract with an adsorbent (B).

3. The method of claim 2 , wherein the adsorbent (B) is at least one selected from the group consisting of an adsorbent comprising activated carbon, a synthetic adsorbent obtained by copolymerizing styrene and divinylbenzene, a phenol-formaldehyde resin adsorbent, an aldehyde adsorbent, magnesium silicate, aluminum hydroxide, aluminum oxide, magnesium oxide, diatomite, activated alumina, silica gel, silica-magnesia gel, an adsorbent obtained by chemically binding an alkyl group or an allyl group on an inorganic carrier, an aromatic adsorbent, a methacrylate ester adsorbent, synthetic zeolite, and a pearlite filter medium.

4. The method of claim 2 , wherein the adsorbent (B) comprises a pearlite filter medium.

5. The method of claim 1 , wherein the contacting comprises contacting the extract with a mixture comprising the adsorbent (A) and an adsorbent (B).

6. The method of claim 5 , wherein the adsorbent (B) is at least one selected from the group consisting of, an adsorbent comprising activated carbon, a synthetic adsorbent obtained by copolymerizing styrene and divinylbenzene, a phenol-formaldehyde resin adsorbent, an aldehyde adsorbent, magnesium silicate, aluminum hydroxide, aluminum oxide, magnesium oxide, diatomite, activated alumina, silica gel, silica-magnesia gel, an adsorbent obtained by chemically binding an alkyl group or an allyl group on an inorganic carrier, an aromatic adsorbent, a methacrylate ester adsorbent, synthetic zeolite, and a pearlite filter medium.

7. The method of claim 5 , further comprising:
separating the extract of the coenzyme Q10-producing microorganism contacted with the adsorbent (A) and the adsorbent (B) from the adsorbent (A) and the adsorbent (B); and
treating the solution comprising the coenzyme Q10 separated from the absorbent (A) and the adsorbent (B).

8. The method of claim 7 , wherein the treating comprises conducting a condensation, a solvent exchange, an oxidation, a reduction, a column chromatography, a crystallization, or a combination thereof.

9. The method of claim 5 , wherein a weight ratio of the adsorbent (A) to the adsorbent (B) in the mixture is from 40: 60 to 60: 40.

10. The method of claim 5 , wherein a weight ratio of the adsorbent (A) to the adsorbent (B) in the mixture is 50: 50.

11. The method of claim 1 , further comprising:
separating the extract contacted with the adsorbent (A) from the adsorbent (A); and
treating the solution comprising the coenzyme Q10 from the absorbent (A).

12. The method of claim 11 , wherein the treating comprises conducting a condensation, a solvent exchange, an oxidation, a reduction, a column chromatography, and a crystallization, or a combination thereof.

13. The method of claim 1 , wherein the adsorbent (A) comprises 60% or more of aluminum silicate.

14. The method of claim 1 , wherein the adsorbent (A) comprises 65% or more of aluminum silicate.

15. The method of claim 1 , wherein the aluminum silicate comprises from 50% to 70% of silicon dioxide.

16. The method of claim 1 , wherein the aluminum comprises from 55% to 65% of silicon dioxide.

17. The method of claim 1 , wherein the aluminum silicate comprises crystal water.

18. The method of claim 1 , wherein the aluminum silicate comprises a solid acid.

19. The method of claim 1 , wherein upon the contacting, the adsorbent (A) absorbs ergosterol and thereby removes ergosterol from the extract, wherein a removal rate of ergosterol is 30% or more.

20. The method of claim 1 , wherein upon the contacting, the adsorbent (A) absorbs ergosterol and thereby removes ergosterol from the extract, wherein a removal rate of ergosterol is 40% or more.

Assignees

• Kaneka Corp

Inventors

• Lee Wan Ying
• Suzuki Yasuyuki
• Kanda Akihisa

Classifications

• C12P7/66 Primary

  containing the quinoid structure · CPC title

• B01J20/22

  comprising organic material · CPC title

• B01D15/00

  Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor · CPC title

• B01J20/26

  Synthetic macromolecular compounds · CPC title

• C12P23/00 Primary

  Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes (containing heterorings C12P17/00) · CPC title