Plant propagule root-growing device

Patent metadata

Field	Value
Publication number	US-10827694-B2
Application number	US-201515537033-A
Country	US
Kind code	B2
Filing date	Dec 21, 2015
Priority date	Dec 19, 2014
Publication date	Nov 10, 2020
Grant date	Nov 10, 2020

Abstract

Devices for directionally placing a plant propagule (2) inside a tubular hollow member (3), including a platform element (20) arranged such that a foldable member (1) can be placed thereon; an actuating dispensing arrangement {90a, 90b, 100) for placing a foldable member (1) on the platform element (20); an arrangement (40)/(40a) for placing a plant propagule (2) on a foldable member (1) placed on the platform element (20); optionally, including a way (150) for identifying an imaginary line (9) on the foldable member (1) stretching through the root forming end (7) to the shoot forming end (8) of the propagule (2) being directionally placed during operation; an actuating folding arrangement {30, 80)/(120/80) for folding the foldable member (1) along said imaginary line (9) to form a folded foldable member (la); an actuating dispensing arrangement (180) for providing a tubular hollow member (3) having a first open end (4); and an actuating placing arrangement {30, 80)/(120/80) for placing said folded foldable member (la) into the tubular hollow member (3) through the first open end (4). Related methods for handling plant propagules, in particular somatic plant embryos.

First claim

The invention claimed is:

1. A plant propagule placing device for directionally placing a plant propagule inside a tubular hollow member, comprising:
a. a platform element arranged such that a foldable member can be placed thereon;
b. an actuating foldable member dispensing arrangement for placing the foldable member on the platform element;
c. an arrangement for placing the plant propagule on the foldable member placed on the platform element;
d. a means for identifying an imaginary line on the foldable member stretching through a root forming end to a shoot forming end of the plant propagule being directionally placed during operation or the arrangement is configured for placing a plant propagule on a foldable member such that the foldable member provides the plant propagule in a known orientation thus identifying the location of the imaginary line;
e. an actuating folding arrangement for folding the foldable member along said imaginary line to form a folded foldable member;
f. an actuating tubular dispensing arrangement for providing the tubular hollow member having a first open end; and
g. an actuating placing arrangement for placing said folded foldable member into the tubular hollow member through the first open end;
wherein the actuating folding arrangement is configured to also function as an actuating placing arrangement;
wherein the device is configured for performing a method for directionally placing the plant propagule inside the tubular hollow member, such that during operation, the device performs the steps of:
I. providing the tubular hollow member having a first open end by means of the actuating, hollow-member-dispensing arrangement;
II. providing the foldable member, and placing the foldable member on the platform element by means of the actuating, foldable-member-dispensing arrangement;
III. placing a plant propagule on said foldable member placed on the platform element by means of an arrangement;
IV. identifying the root forming end and the shoot forming end of the plant propagule and identifying the imaginary line across the foldable member stretching through the root forming end to the shoot forming end of the plant propagule, by the means for identifying an imaginary line;
V folding the foldable member along said line by means of the actuating folding arrangement such that the plant propagule becomes wrapped in the folded foldable member; and
VI. placing said folded foldable member with the wrapped plant propagule into the tubular hollow member through the first open end by means of the actuating placing arrangement.

2. The device according to claim 1 , wherein the platform element is rotatable along an axis.

3. The device according to claim 1 , wherein the platform element is arranged on a support element rotatable along an axis.

4. The device according to claim 1 , comprising four or more platform elements.

5. The device according to claim 1 , wherein the actuating foldable member dispensing arrangement for placing the foldable member on the platform element comprises a suction device placed on an actuator wherein the suction device is configured for picking up the foldable member and placing the foldable member on top of the platform element during operation.

6. The device according to claim 1 , wherein the arrangement for placing a plant propagule on the foldable member placed on the platform element comprises:
a. a delivery conduit capable of delivering a stream of fluid comprising plant propagules suspended in the fluid, wherein the delivery conduit is located above the platform element;
b. a collection conduit capable of collecting the stream of fluid;
c. a detector for detecting the plant propagule in the stream of fluid in the delivery conduit;
d. an actuating means for reversibly diverting the collection conduit when the plant propagule is detected, operatively connected to the detector for detecting the plant propagule;
wherein the device is configured such that in operation, the actuating foldable member dispensing arrangement for placing the foldable member on the platform element places the foldable member on the platform element; and
when the plant propagule is detected in the stream of fluid in the delivery conduit, the actuating means for diverting the collection conduit is activated by the detector for detecting the plant propagule such that the stream of fluid from the delivery conduit places the plant propagule on the foldable member, after which the diversion of the collection conduit is reverted by the actuating means in preparation of a second propagule.

7. The device according to claim 1 , wherein the arrangement for placing the plant propagule on the foldable member placed on the platform element comprises:
a conduit having a first outlet and a second outlet capable of delivering a stream of fluid comprising plant propagules suspended in the fluid, wherein the second outlet of the conduit is located above the platform element, and wherein the first outlet is arranged such that the first outlet may be provided with interruptible suction during operation; and
a detector for detecting the plant propagule in the stream of fluid in the conduit;
wherein the device is configured such that during operation, the actuating foldable member dispensing arrangement for placing the foldable member on the platform element places the foldable member on the platform element;
wherein, in a default state, the first outlet is provided with a suction such that the stream of fluid is prevented from exiting through the second outlet; and
wherein when the plant propagule is detected in the stream of fluid in the delivery conduit, the suction through the first outlet is interrupted for a time such that the plant propagule exits through the second outlet with the stream of fluid and becomes placed onto the foldable member, after which the suction through the first outlet is resumed.

8. The device according to claim 1 , wherein the actuating folding arrangement for folding the foldable member along said imaginary line comprises a sliding linear actuator and a folding platform for the foldable member.

9. The device according to claim 1 , wherein said means for identifying the imaginary line on the foldable member comprises a digital imaging arrangement for identifying the root forming end and the shoot forming end of the plant propagule and for identifying an imaginary line on the foldable member stretching through the root forming end to the shoot forming end of the plant propagule.

10. The device according to claim 1 , wherein the actuating tubular hollow member dispensing arrangement for providing the tubular hollow member comprises linear actuators having the capacity of moving in different directions during operation.

11. The device according to claim 1 , wherein the actuating placing arrangement for placing said folded foldable member into the tubular hollow member through the first open end such that the root forming end of the plant propagule is directed away from the first end of said tubular hollow member comprises a gripping and sliding actuation mechanism and a sliding linear actuator, wherein the device is configured such that during operation, the following is performed by the device:
a. the actuator is moved to become located on top of the foldable member with a plant propagule;
b. the gripping and sliding actuation mechanism is moved to fold the foldable member along the imaginary line; and
c. the folded foldable member is held in place by the sliding linear actuator and the gripping and sliding actuation mechanism which move the folded foldable member into the tubular hollow member, after which the sliding linear actuator is lifted

Assignees

• Georgia Tech Res Inst

Inventors

• Aidun Cyrus

Classifications

• A01H4/00

  Plant reproduction by tissue culture techniques {; Tissue culture techniques therefor} · CPC title

• A01H4/001 Primary

  Culture apparatus for tissue culture · CPC title

• C12N5/04

  Plant cells or tissues {(culture media C12N5/0025)} · CPC title

• C12M1/00

  Apparatus for enzymology or microbiology · CPC title

• A01H4/005 Primary

  Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques · CPC title