Methods and compositions for weed control

Patent metadata

Field	Value
Publication number	US-10808249-B2
Application number	US-201213612954-A
Country	US
Kind code	B2
Filing date	Sep 13, 2012
Priority date	Sep 13, 2011
Publication date	Oct 20, 2020
Grant date	Oct 20, 2020

Abstract

Provided are novel compositions for use to enhance weed control. Specifically, the present invention provides for methods and compositions that modulate Phytoene desaturase in weed species. The present invention also provides for combinations of compositions and methods that enhance weed control.

First claim

We claim:

1. A method of plant control comprising:
topically applying to a surface of a plant a composition comprising a non-transcribable polynucleotide and a transfer agent,
wherein said non-transcribable polynucleotide is from 21 to about 700 nucleotides in length and comprises a nucleotide sequence identical or complementary to at least 21 contiguous nucleotides of a phytoene desaturase (PDS) gene sequence or an RNA sequence thereof selected from the group consisting of SEQ ID NOs: 13, 15, 24, 35, 37, 41, 43-46, 50-56, 58-60, 64, 70-72, 74-80, 89-92, 123, 124, 165-170, 173-178, 185-194, 199-202, 207-212, 223-234, 241-248, 251, 252, 255-258, 265-270, 275-280, 305-310, 313-332, 337-342, 353-364, 367-370, 377-380, 391-404, 411-424, 427, 428, 437-442, 445-518, 521-526, 533-536, 547, 548, 551-554, 561-570, 575, 576, 583-596, 601, 602, 609-614, 619-626, 643, 644, 669-674, 681, 682, 719-722, 727-730, 733-748, 753-756, 759-762, 769, 770, 775-778, 781-784, 787-792, 797, 798, 801, 802, 811-814, 823-842, 845, 846, 851, 852, 855, 856, 861-868, 871-876, 885, 886, 893-1004, 1015, 1016, 1021-1042, 1045, 1046, 1049-1070, 1073, 1074, 1081, 1082, 1085-1092, 1097-1104, 1109-1230, 1233, 1234, 1237-1258, 1261, 1262, 1269, 1270, 1273-1282, 1285-1294, 1297-1310, 1313-1328, 1331-1334, 1337-1340, 1343-1398, 1403-1408, 1413-1460, 1463-1476, 1479-1536, 1539-1560, 1565-1576, 1579-1606, 1609-1634, 1637-1640, 1643, 1644, 1647-1652, 1655-1670, 1675-1678, 1681-1684, 1689-1756, 1759-1784, 1787-1790, 1797-1800, 1803-1820, 1823-1828, 1831, 1832, 1837-1856, 1859-1862, 1867, 1868, 1871, 1872, 1881-1884, 1893, 1894, 1901-1974, 1977-1984, 1987-1990, 1993-2010, 2048, 2052, 2075, 2083, 2085, 2090, 2100, 2109, 2117, 2126, 2131, 2135, and 2138-2176,
wherein said transfer agent is a surfactant and conditions said surface of said plant for permeation by said non-transcribable polynucleotide,

whereby said plant's growth, development, or reproductive ability is suppressed or delayed or said plant is more sensitive to a PDS inhibitor herbicide, relative to an untreated plant.

2. The method as claimed in claim 1 , wherein said transfer agent comprises an organosilicone surfactant composition or an organosilicone compound contained therein.

3. The method as claimed in claim 1 , wherein said non-transcribable polynucleotide is selected from the group consisting of a sense single-stranded DNA (ssDNA), an anti-sense ssDNA, a sense single-stranded RNA (ssRNA), an anti-sense ssRNA, a double-stranded RNA (dsRNA), a double-stranded DNA (dsDNA), and a dsDNA/RNA hybrid.

4. The method as claimed in claim 1 , wherein said plant is selected from the group consisting of Abutilon theophrasti, Amaranthus chlorostachys, Amaranthus graecizans, Amaranthus palmeri, Amaranthus rudis, Amaranthus hybridus, Amaranthus lividus, Amaranthus spinosus, Amaranthus viridis, Ambrosia artemisiifolia, Ambrosia trifida, Commelina diffusa, Conyza canadensis, Digitaria sanguinalis, Euphorbia heterophylla, Kochia scoparia, Lolium multiflorum, Taraxacum officinale , and Lactuca serriola.

5. The method as claimed in claim 1 , wherein said composition further comprises said PDS inhibitor herbicide.

6. The method as claimed in claim 5 , wherein said composition further comprises one or more co-herbicides similar to or different from said PDS inhibitor herbicide.

7. The method as claimed in claim 1 , wherein said composition comprises any combination of two or more of said non-transcribable polynucleotides.

8. A composition for topical application to a surface of a plant comprising a non-transcribable polynucleotide and a transfer agent,
wherein said non-transcribable polynucleotide is from 21 to about 700 nucleotides in length and comprises a nucleotide sequence identical or complementary to at least 21 contiguous nucleotides of a phytoene desaturase (PDS) gene sequence or an RNA sequence thereof selected from the group consisting of SEQ ID NOs: 13, 15, 24, 35, 37, 41, 43-46, 50-56, 58-60, 64, 70-72, 74-80, 89-92, 123, 124, 165-170, 173-178, 185-194, 199-202, 207-212, 223-234, 241-248, 251, 252, 255-258, 265-270, 275-280, 305-310, 313-332, 337-342, 353-364, 367-370, 377-380, 391-404, 411-424, 427, 428, 437-442, 445-518, 521-526, 533-536, 547, 548, 551-554, 561-570, 575, 576, 583-596, 601, 602, 609-614, 619-626, 643, 644, 669-674, 681, 682, 719-722, 727-730, 733-748, 753-756, 759-762, 769, 770, 775-778, 781-784, 787-792, 797, 798, 801, 802, 811-814, 823-842, 845, 846, 851, 852, 855, 856, 861-868, 871-876, 885, 886, 893-1004, 1015, 1016, 1021-1042, 1045, 1046, 1049-1070, 1073, 1074, 1081, 1082, 1085-1092, 1097-1104, 1109-1230, 1233, 1234, 1237-1258, 1261, 1262, 1269, 1270, 1273-1282, 1285-1294, 1297-1310, 1313-1328, 1331-1334, 1337-1340, 1343-1398, 1403-1408, 1413-1460, 1463-1476, 1479-1536, 1539-1560, 1565-1576, 1579-1606, 1609-1634, 1637-1640, 1643, 1644, 1647-1652, 1655-1670, 1675-1678, 1681-1684, 1689-1756, 1759-1784, 1787-1790, 1797-1800, 1803-1820, 1823-1828, 1831, 1832, 1837-1856, 1859-1862, 1867, 1868, 1871, 1872, 1881-1884, 1893, 1894, 1901-1974, 1977-1984, 1987-1990, 1993-2010, 2048, 2052, 2075, 2083, 2085, 2090, 2100, 2109, 2117, 2126, 2131, 2135, and 2138-2176,
wherein said transfer agent is a surfactant and conditions said surface of said plant for permeation by said non-transcribable polynucleotide, and
whereby said plant treated with said composition has its growth, development, or reproductive ability suppressed or delayed or said plant is more sensitive to a PDS inhibitor herbicide, relative to an untreated plant.

9. The composition of claim 8 , wherein said transfer agent is an organosilicone composition or an organosilicone compound contained therein.

10. The composition of claim 8 , wherein said non-transcribable polynucleotide is selected from the group consisting of SEQ ID NOs: 24, 79, 80, 89-92, 123, 124, 185-194, 199-202, 207-212, 223-234, 241-246, 265, 266, 269, 270, 275-280, 305-310, 313-318, 323-332, 337-342, 355-362, 367-370, 377-380, 391-404, 411-418, 441, 442, 445-454, 515-518, 521-526, 533-536, 547, 548, 551-554, 609-614, 619-626, 643, 644, 669-672, 681, 682, 719-722, 727-730, 733-736, 747, 748, 753-756, 759-762, 769, 770, 775-778, 781-784, 787, 788, 801, 802, 873-876, 885, 886, 893-896, 907, 908, 999-1004, 1015, 1016, 1021-1024, 1279, 1280, 1473-1476, 1483-1494, 1981-1984, 1987-1990, 1993, and 1994.

11. The composition of claim 8 , wherein said non-transcribable polynucleotide is selected from the group consisting of SEQ ID NOs: 2048, 2052, 2075, 2083, 2085, 2090, 2100, 2109, 2117, 2126, 2131, and 2135.

12. The composition of claim 8 , further comprising a PDS inhibitor herbicide.

13. The composition of claim 12 , wherein said PDS inhibitor herbicide is selected from the group consisting of pyridazinones, pyridinecarboxamides, beflubutamid, fluridone, flurochloridone, and flurtamone.

14. The composition of claim 12 , further comprising a co-herbicide.

15. An agricultural chemical composition for topical application to a surface of a plant comprising a non-transcribable polynucleotide, a phytoene desaturase (PDS inhibitor herbicide, a transfer agent, and a co-herbicide,
wherein said non-transcribable polynucleotide is from 21 to about 700 nucleotides in length and comprises a nucleotide sequence identical or complementary to at least 21 contiguous nucleotides of a PDS gene sequence or an RNA sequence thereof selected from the group consisting of SEQ ID NOs: 13, 15, 24, 15, 37, 41, 43-46, 50-56, 58-60, 64, 70-72, 74-80, 89-92, 123, 124, 165-170, 173-178, 185-194, 199-202, 207-212, 223-234, 241-248, 251, 252, 255-258, 265-270, 275-280, 305-310, 313-332, 337-342, 353-364, 367-370, 377-

Assignees

• Ader Daniel
• Finnessy John J
• Li Zhaolong
• Liu Hong
• Masucci James D
• Shah Ronak Hasmukh
• Tao Nengbing
• Wang Dafu
• Monsanto Technology Llc

Inventors

• Ader Daniel
• Finnessy John J
• Li Zhaolong
• Liu Hong
• Masucci James D
• Shah Ronak Hasmukh
• Tao Nengbing
• Wang Dafu

Classifications

• C12N9/0004 Primary

  Oxidoreductases (1.) · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• A01N43/58

  1,2-Diazines; Hydrogenated 1,2-diazines · CPC title

• A01N57/16

  containing heterocyclic radicals · CPC title

• C12N15/1137 Primary

  against enzymes (viral enzymes C12N15/1131; receptors C12N15/1138) · CPC title