Methods and Compositions for Weed Control

Patent metadata

Field	Value
Publication number	US-2016015035-A1
Application number	US-201414774427-A
Country	US
Kind code	A1
Filing date	Mar 11, 2014
Priority date	Mar 13, 2013
Publication date	Jan 21, 2016
Grant date	—

Abstract

The present invention provides novel compositions for use to enhance weed control. Specifically, the present invention provides for methods and compositions that modulate gene expression in Lolium . The present invention also provides for combinations of compositions and methods that enhance Lolium control.

First claim

We claim:

1 . A method of Lolium weed species plant control comprising: treating a Lolium weed species plant or a part of said plant in need of control with a first herbicidal composition comprising a polynucleotide, an organosilicone surfactant concentration of about 0.2 percent or greater, and an effective dose of a nonpolynucleotide herbicide, wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidium gene polynucleotide selected from the group consisting of SEQ ID NO: 1-66, wherein said treated plant is more sensitive to said nonpolynucleotide herbicide relative to a similar plant treated with a second herbicidal composition not containing said polynucleotide.

2 . The method of claim 1 , wherein said Lolium weed species is selected from the group consisting of Lolium rigidum, Lolium canariense, Lolium edwardii, Lolium multiflorum, Lolium perenne, Lolium persicum, Lolium remotum and Lolium temulentum.

3 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 1-10 and said nonpolynucleotide herbicide is selected from the group consisting of aryloxyphenoxypropionates, cyclohexanediones and phenylpyrazoline.

4 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 11-21 and said nonpolynucleotide herbicide is selected from the group consisting of sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinyl(thio)benzoates, and sulfonylaminocarbonyl-triazolinones.

5 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 22-27 and said nonpolynucleotide herbicide is selected from the group consisting of sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinyl(thio)benzoates, and sulfonylaminocarbonyl-triazolinones.

6 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 28-32 and said nonpolynucleotide herbicide is selected from the group consisting of sulfonamides and asulam.

7 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 33-37 and said nonpolynucleotide herbicide is glyphosate.

8 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 38-45 and said nonpolynucleotide herbicide is glufosinate.

9 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 46-50 and said nonpolynucleotide herbicide is selected from the group consisting of triketones, isoxazoles, and pyrazoles.

10 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 51-56 and said nonpolynucleotide herbicide is selected from the group consisting of pyridazinones, pyridinecarboxamides, beflubutamid, fluridone, flurochloridone and flurtamone.

11 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides in length and essentially identical or essentially complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 57-66 and said nonpolynucleotide herbicide is selected from the group consisting of acifluorfen-Na, bifenox, chlomethoxyfen, fluoroglycofen-ethyl, fomesafen, halosafen, lactofen, oxyfluorfen, fluazolate, pyraflufen-ethyl, cinidon-ethyl, flumioxazin, flumiclorac-pentyl, fluthiacet-methyl, thidiazimin, oxadiazon, oxadiargyl, azafenidin, carfentrazone-ethyl, sulfentrazone, pentoxazone, benzfendizone, butafenacil, pyrazogyl, and profluazol.

12 . The method of claim 1 , wherein said polynucleotide is selected from the group consisting of sense or anti-sense ssDNA or ssRNA, dsRNA, or dsDNA, or dsDNA/RNA hybrids.

13 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous nucleotides, 20 contiguous nucleotides or 21 contiguous nucleotides in length and at least 85 percent identical or complementary to a segment of said Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO:1-66.

14 . The method of claim 1 , wherein said polynucleotide is at least 19 contiguous polynucleotides selected from the group consisting of SEQ ID NO: 67-155.

15 . The method of claim 1 , wherein said polynucleotide is at least 85 percent homologous or complementary to polynucleotides selected from the group consisting of SEQ ID NO: 67-155.

16 . The method of claim 1 , wherein said herbicidal composition comprises any combination of two or more of said polynucleotides, wherein said polynucleotide is at least 19 polynucleotides in length and at least 85 percent identical or complementary to a segment of a Lolium rigidum gene polynucleotide selected from the group consisting of SEQ ID NO: 1-66, and said herbicidal composition comprises any combination of two or more nonpolynucleotide herbicides that inhibit the activity of two or more proteins selected from the group consisting of ACCase, ALS large subunit, ALS small subunit, DHPS, EPSPS, GS2, HPPD, PDS, PPDX, and wherein said treated plant is more sensitive to the two or more nonpolynucleotide herbicides relative to a similar plant treated with a herbicidal composition not containing said polynucleotides.

17 . The method of claim 1 , wherein said herbicidal composition further comprises one or more nonpolynucleotide herbicide selected from the group consisting of: 5-Diarylpyrazole herbicides, 2-Thiopyrimidine herbicides, 3-CF3-Benzene herbicides, Acetamide herbicides, Amide herbicides, Aminoacrylate herbicides, Aminotriazine herbicides, Aromatic acid herbicides, Arsenical herbicides, Arylaminopropionic acid herbicides, Arylcarboxamide herbicides, Arylcyclodione herbicides, Aryloxyphenoxy-propionate herbicides, Azolecarboxamide herbicides, Azoloazinone herbicides, Azolotriazine herbicides, Benzamide herbicides, Benzenesulfonamide herbicides, Benzhydryl herbicides, Benzimidazole herbicides, Benzofuran herbicides, Benzofuranyl Alkylsulfonate herbicides, Benzohydrazide herbicides, Benzoic acid herbicides, Benzophenylmethanone herbicides, Benzothiadiazinone herbicides, Benzothiazole herbicides, Benzothiazoleacetate herbicides, Benzoxazole herbicides, Benzoylcycloh

Assignees

• Monsanto Technology Llc

Inventors

• Tao Nengbing

Classifications

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• A01N25/30

  characterised by the surfactants · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• A01N57/16 Primary

  containing heterocyclic radicals · CPC title