Apparatus for detecting ATP in a liquid sample

Patent metadata

Field	Value
Publication number	US-10793890-B2
Application number	US-201314410718-A
Country	US
Kind code	B2
Filing date	Jul 1, 2013
Priority date	Jul 6, 2012
Publication date	Oct 6, 2020
Grant date	Oct 6, 2020

Abstract

An apparatus (1000) and kit (1000) for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having (100) a sampling portion (30) and a handling portion (20). The sampling portion (30) is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity (32) that is not substantially defined by space between a plurality of fibers. The sampling device (30) comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.

First claim

What is claimed is:

1. An apparatus, comprising:
a container with an opening and a cuvette portion that is adapted to be operationally coupled to a luminometer;
a liquid reagent composition comprising luciferin, wherein the liquid reagent composition is disposed in a closed compartment, wherein the pH of the liquid reagent composition is about 6.8 or lower; and
a sampling device having a sampling portion;
wherein the sampling portion is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity that is not substantially defined by space between a plurality of fibers;
wherein the sampling device comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with the liquid reagent composition, changes the pH of the liquid reagent composition to 6.9 or higher.

2. The apparatus of claim 1 , wherein the dry coating is disposed on the sampling portion of the sampling device.

3. The apparatus of claim 1 , wherein the pH-adjusting reagent comprises a water-soluble reagent.

4. The apparatus of claim 1 , wherein the sampling portion comprises a calibrated loop.

5. The apparatus of claim 1 , wherein the closed compartment comprises a frangible wall.

6. The apparatus of claim 5 , wherein the frangible wall is disposed in the container between the opening and the cuvette portion.

7. The apparatus of claim 1 , wherein the sampling device comprises the closed compartment, wherein the sampling device further comprises a structure that selectively releases the fluid composition from the container.

8. The apparatus of claim 1 , wherein the sampling portion comprises a foam material.

9. The apparatus of claim 1 , wherein the pH-adjusting reagent comprises a buffer component selected from the group consisting of N-[2-hydroxyethyl] piperazine-N′-[2-ethanesulfonic acid], N-[tris(hydroxymethyl)methyl]glycine, and combinations thereof.

10. The apparatus of claim 1 , wherein the pH-adjusting reagent is coated in the one or more cavity.

11. The apparatus of claim 1 , wherein the coating further comprises a cell extractant.

12. The apparatus of claim 1 , wherein the coating does not comprise an amount of a phosphate buffer component effective to change the pH of the liquid reagent composition to a pH of 6.9 or higher.

13. The apparatus of claim 1 , wherein the predetermined volume is about 0.01 milliliters to about 0.25 milliliters.

14. A method, comprising:
using a sampling device to obtain a predetermined volume of a liquid sample;
wherein the sampling device comprises a sampling portion;
wherein the sampling portion is adapted to acquire and releasably retain a predetermined volume of liquid sample in one or more cavity, wherein the one or more cavity is not substantially defined by space between a plurality of fibers;
wherein the sampling device comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher;
wherein the liquid reagent composition comprises a luciferin;

contacting the predetermined volume of sample and the pH-adjusting reagent with a liquid reagent composition in a container to form a reaction mixture; and
using a luminometer to detect light emitted from the reaction mixture;
wherein the container comprises a cuvette portion adapted to be operationally coupled to the luminometer.

15. The method of claim 14 , further comprising the step of agitating the predetermined volume of the sample and the liquid reagent composition in the container.

16. The method of claim 14 , wherein the liquid reagent composition further comprises a luciferase enzyme.

17. The method of claim 14 , wherein contacting the sampling device comprising the sample with liquid reagent composition comprises contacting the sampling device with the liquid reagent composition at a temperature within a range of 10° C. and 35° C., inclusive.

18. The method of claim 14 , wherein, after contacting the sampling device with liquid reagent composition, a substantially steady-state amount of light is emitted from the composition in less than 20 seconds.

Assignees

• 3M Innovative Properties Co

Inventors

• Davenport Kenneth R
• Driscoll Mark B
• Evans Rachel E
• Ramsay Catherine M
• Percy Neil

Classifications

• G01N1/02

  Devices for withdrawing samples {(sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08)} · CPC title

• G01N2001/028

  Sampling from a surface, swabbing, vaporising · CPC title

• C12Q1/66

  involving luciferase · CPC title

• C12Q1/008 Primary

  for determining co-enzymes or co-factors, e.g. NAD, ATP · CPC title

• B01L3/5029

  using swabs · CPC title