Culture container and cell culturing method and cell observation method using culture container

Patent metadata

Field	Value
Publication number	US-10793818-B2
Application number	US-201615753091-A
Country	US
Kind code	B2
Filing date	Aug 19, 2016
Priority date	Aug 20, 2015
Publication date	Oct 6, 2020
Grant date	Oct 6, 2020

Abstract

The purpose of the present invention is to provide a culture container capable of preventing a concave meniscus without using a jig. In order to achieve this purpose, the present invention provides a culture container ( 1 A) provided with: a base ( 3 ) having a concave portion ( 4 ); and a water-repellent layer ( 5 ) formed on an outer edge region ( 411 ) of the bottom ( 41 ) of the concave portion ( 4 ) and the inner circumferential surface ( 42 ) of the concave portion ( 4 ). One of the surfaces of the water-repellent layer ( 5 ) is exposed to the space in the concave portion ( 4 ).

First claim

The invention claimed is:

1. A culture container, comprising:
a base having a concave portion formed therein;
a water repellent layer formed only on an outer edge region of a bottom surface of the concave portion and an inner circumferential surface of the concave portion;
and a central region having hydrophilicity and located inward of the outer edge region of the bottom surface of the concave portion,
wherein the outer edge region is formed in an annular shape along an outer peripheral line of the bottom surface of the concave portion,
wherein one surface of the water repellent layer is exposed to an internal space of the concave portion.

2. The culture container of claim 1 , wherein the bottom surface of the concave portion is a flat surface.

3. The culture container of claim 1 , wherein a contact angle between the one surface of the water repellent layer and water is 115 degrees or more.

4. A culture container, comprising:
a base having a concave portion formed therein;
a water repellent layer formed on an outer edge region of a bottom surface of the concave portion and an inner circumferential surface of the concave portion;
a central region having hydrophilicity and located inward of the outer edge region of the bottom surface of the concave portion; and
a DLC layer formed at a side of the other surface of the water repellent layer,
wherein the outer edge region is formed in an annular shape along an outer peripheral line of the bottom surface of the concave portion,
and wherein one surface of the water repellent layer is exposed to an internal space of the concave portion.

5. The culture container of claim 4 , further comprising:
an adhesion layer formed between the water repellent layer and the DLC layer.

6. A method of culturing cells using the culture container of claim 1 , comprising:
forming an extracellular matrix layer in a region other than the outer edge region in the bottom surface of the concave portion, before seeding the cells in the concave portion.

7. A method of observing cells using the culture container of claim 1 , comprising:
observing the cells in the concave portion with a phase difference microscope from a side of the bottom surface of the concave portion or a side of an opening of the concave portion.

Assignees

• Tokyo Electron Ltd
• Tocalo Co Ltd

Inventors

• Gomi Shinichi
• Kagawa Kenichi
• Yoda Yusuke
• Miki Shinya
• Hamaguchi Tatsuya

Classifications

• G02B21/34 Primary

  Microscope slides, e.g. mounting specimens on microscope slides · CPC title

• G01N2021/0378

  Shapes · CPC title

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12M23/10

  Petri dish (crystallising dishes B01L3/06) · CPC title

• G02B21/14

  affording illumination for phase-contrast observation · CPC title